A survey of natural parasitism of mirid bugs by parasitoids on apples and pears in Norway

Mirid bugs are important pests in both integrated and organic fruit production. In a survey in 2006 the proportion of mirids parasitized by parasitoids was investigated in 18 Norwegian orchards. More than 50 beating samples were collected from apple and pear orchards under integrated and organic management in different areas of Norway. About 30% of nymphs of the most abundant mirid bug species, Lygocoris pabulinus, Ortholylus marginalis and Psallus ambiguus, were parasitized. Important factors for enhancing biological control in organic fruit production are discussed

Control methods against bugs (Hemiptera; Heteroptera) in organic apple and pear production

True bugs (Hemiptera; Heteroptera) are important pests in fruit production in Norway. In organic fruit pro-duction they may damage up to 40% of the crop. Several of the Heteropteran species attacking apple and pear are polyphagous, with many other hostplants than pome fruit. In organic production few control meth-ods are available against bugs. In this study we have tried a cultural control method (mowing of ground-cover) as well as spray applications of various biological insecticides against bugs. Spray application of azadirachtin (NeemAzal), rape oil and garlic extract (Ecoguard) were tested. Results indicate that the effect of mowing groundcover inside the orchard is variable and small. Applying NeemAzal reduced the number of bugs and damage. Oil and Ecoguard showed little effect in this study, however further testing is needed. Results from the first two years of the study will be discussed in relation to both cultural and direct control methods against bugs

Neurons are MHC Class I-Dependent Targets for CD8 T Cells upon Neurotropic Viral Infection

Following infection of the central nervous system (CNS), the immune system is faced with the challenge of eliminating the pathogen without causing significant damage to neurons, which have limited capacities of renewal. In particular, it was thought that neurons were protected from direct attack by cytotoxic T lymphocytes (CTL) because they do not express major histocompatibility class I (MHC I) molecules, at least at steady state. To date, most of our current knowledge on the specifics of neuron-CTL interaction is based on studies artificially inducing MHC I expression on neurons, loading them with exogenous peptide and applying CTL clones or lines often differentiated in culture. Thus, much remains to be uncovered regarding the modalities of the interaction between infected neurons and antiviral CD8 T cells in the course of a natural disease. Here, we used the model of neuroinflammation caused by neurotropic Borna disease virus (BDV), in which virus-specific CTL have been demonstrated as the main immune effectors triggering disease. We tested the pathogenic properties of brain-isolated CD8 T cells against pure neuronal cultures infected with BDV. We observed that BDV infection of cortical neurons triggered a significant up regulation of MHC I molecules, rendering them susceptible to recognition by antiviral CTL, freshly isolated from the brains of acutely infected rats. Using real-time imaging, we analyzed the spatio-temporal relationships between neurons and CTL. Brain-isolated CTL exhibited a reduced mobility and established stable contacts with BDV-infected neurons, in an antigen- and MHC-dependent manner. This interaction induced rapid morphological changes of the neurons, without immediate killing or impairment of electrical activity. Early signs of neuronal apoptosis were detected only hours after this initial contact. Thus, our results show that infected neurons can be recognized efficiently by brain-isolated antiviral CD8 T cells and uncover the unusual modalities of CTL-induced neuronal damage

The strawberry blossom weevil : simulation of damage

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The strawberry blossom weevil : simulation of damage

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Biological control of mites on strawberry grown under protection and in the open field

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Pollinator communities in strawberry crops – variation at multiple spatial scales

Predicting potential pollination services of wild bees in crops requires knowledge of their spatial distribution within fields. Field margins can serve as nesting and foraging habitats for wild bees and can be a source of pollinators. Regional differences in pollinator community composition may affect this spill-over of bees. We studied how regional and local differences affect the spatial distribution of wild bee species richness, activity-density and body size in crop fields. We sampled bees both from the field centre and at two different types of semi-natural field margins, grass strips and hedges, in 12 strawberry fields. The fields were distributed over four regions in Northern Europe, representing an almost 1100 km long north-south gradient. Even over this gradient, daytime temperatures during sampling did not differ significantly between regions and did therefore probably not impact bee activity. Bee species richness was higher in field margins compared with field centres independent of field size. However, there was no difference between centre and margin in body-size or activity-density. In contrast, bee activity-density increased towards the southern regions, whereas the mean body size increased towards the north. In conclusion, our study revealed a general pattern across European regions of bee diversity, but not activity-density, declining towards the field interior which suggests that the benefits of functional diversity of pollinators may be difficult to achieve through spill-over effects from margins to crop. We also identified dissimilar regional patterns in bee diversity and activity-density, which should be taken into account in conservation management

Status of sustainable strawberry production within Europe

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