Quantitative analysis of chemical constituents in medicinal plant coleus aromaticus extracts

Background: Indian medicinal plants have great potential towards curing many diseases. Medicinal plant Coleus aromaticus is known for its wide medical applications. The main objectives of the study undertaken were to analyze the phytochemicals and compare the concentration present in the dialyzed Coleus aromaticus protein extract with various solvents.Methods: Various extracts of the medicinal plant Coleus aromaticus leaves such as dialyzed Coleus aromaticus protein, hydro alcoholic extract, ethanol extract and chloroform extract were prepared and analyzed for various phytochemical concentrations such as Total phenols (Folin-Ciocalteu method), Flavonoids (Aluminum chloride method), total sugars (Dubois method) and Proteins (Bradford’s method). Standard graph for each component was plotted.Results: The protein concentration in dialyzed Coleus aromaticus protein extract is considerably higher (5.8μg/10μl) than hydro alcoholic extract (1.6μg/10μl), ethanol extract (5.2μg/10μl) and chloroform extract (2.8μg/10μl). The other phytochemicals like total phenol, flavonoids and total sugars were low in concentration in the dialyzed Coleus aromaticus protein extract compared to hydro alcoholic extract, ethanol extract and chloroform extract.Conclusions: In this study, the protein concentration in dialyzed Coleus aromaticus protein extract is considerably higher than the same in other solvent extracts. This is the basis for further studies to unfold the antioxidant activity of Coleus aromaticus protein in vitro

Portulaca oleracea inhibit vincristine induced peripheral neuropathy: involvement of ATP-sensitive K+ channels

Background: The Portulaca oleracea belonging to portulacaceae family. It is a herbaceous plant widely distributed throughout the world and used in traditional medicine for many ailments. The present study was to evaluate the antinociceptive action of petroleum ether extract of P. oleracea in vincristine induced peripheral neuropathic pain and the possible mechanisms involved.Methods: Peripheral neuropathy was induced in rats by administration of vincristine sulfate (50 μg/kg i.p.) for 10 consecutive days. The cold tail hyperalgesia was assessed by cold water tail immersion test. To identify the possible mechanisms involved in the antinociceptive action of petroleum ether extract of P. oleracea, acetic acid writhing method was employed. Mice were pretreated with naloxone, glibenclamide before petroleum ether extract treatment to identify the involvement of opioid and potassium channels, respectively.Results: The administration of petroleum ether extract of P. oleracea (100 and 200 mg/kg p.o.) for 10 days significantly attenuated vincristine-induced cold hyperalgesia. Pre-treatment with glibenclamide reversed the antinociceptive effect of P. oleracea, but the naloxone pre-treatment did not reverse the antinociceptive activity of P. oleracea.Conclusion: The results of the present study reveal the antinociceptive effect of P. oleracea in vincristine induced peripheral neuropathy and involving ATP-sensitive potassium channels pathway

INLINE ELASTICITY MEASURE OF TEXTILE USING ANN

In this paper, we propose a technique to measure the Elasticity Modulus of the textile material using flex LVDT. Elasticity modulus is measured indirectly by measuring stiffness of the material first. The material whose stiffness is to be measured is subjected to a known force and the deflection caused in the material due to applied load is measured using the LVDT. Here the whole measurement is done dynamically without halting the manufacturing line of process. The output of LVDT is AC voltage. AC-DC converter is used to convert the AC output voltage of LVDT to DC output voltage. This is cascaded to the ANN block programmed on the LabVIEW platform. The results show that the proposed technique has achieved its proposed objectives

A randomized open label comparative study to determine the various side effects and patient satisfaction of low dose continuous versus low dose intermittent oral isotretinoin therapy in moderate to severe acne vulgaris

Background: Acne Vulgaris is chronic inflammatory disease of pilosebaceous units. Oral isotretinoin is recommended for moderate to severe acne vulgaris who are not responding satisfactorily to conventional therapies. Recent reports indicate that acne patients have been benefiting from the low dose treatment protocols. However, long term daily use of this drug results in frequent side effects such mucocutaneous and systemic side effects. Our aim was to assess and compare the various side effects and patient satisfaction of oral isotretinoin in low dose continuous and intermittent treatment of moderate to severe acne vulgaris.Methods: This was a prospective randomized open labeled comparative study carried out at outpatient department in the Department of Dermatology in Mandya Institute of Medical sciences, Mandya. Patients with moderate to severe acne were assigned equally (50 subjects each) to one of the two treatment regimens by using block randomization technique, Group A was given low dose continuous regimen-20 mg oral isotretinoin once daily for 4 months and Group B was given low dose intermittent regimen-20 mg oral isotretinoin once daily for 1 week out of every 4 weeks. The patients were followed up every 4th week during the treatment period. The patients were examined and side effects were noted in each visit. A six month follow-up evaluation was done to analyze patient satisfaction.Results: Muco-cutaneous dryness was most common adverse effect noted in both the groups A and B. Itching (42%), Alopecia (44%), Myalgia (36%) were seen most commonly in group A and Acne flaring (47%) was most common with group B. With regard to patient satisfaction, in group A 42% were satisfied and 20% were very satisfied, in group B 36% were satisfied and 14% were very satisfied.Conclusions: Study suggests that, Muco-cutaneous dryness was most common side effect in both treatment regimens. Side effects were more frequent with low dose continuous than low dose intermittent isotretinoin regimen. Patient satisfaction was better in continuous regimen

Primary oral malignant melanoma - A case report

Melanoma arising from mucosal surfaces of the head and neck is a very rare neoplasm and it is considered among the most deadly of all human neoplasms. Although skin melanomas comprise 1.3% of all cancers, oral malignant melanoma accounts for only 0.2 to 8% of all reported melanomas, common sites of occurrence being palate and maxillary gingiva. Due to its presence at relatively obscure areas in the oral cavity, most oral malignant melanomas are diagnosed at a late stage and are associated with poor prognosis. Early diagnosis is essential for successful treatment and perhaps the key factor in improving the prognosis of oral malignant melanoma. This paper reports a case of a 42year old woman with primary malignant melanoma at a rare site, the left retromolar region involving the left side of the mandible, up to level IV ipsilateral cervical lymph nodes. The patient was treated with left hemimandibulectomy with radical neck dissection and was followed up for two years and 3months without any local recurrence or distant metastasis. It is suggested that any pigmented lesion that are suspected of undergoing changes in their clinical appearance should be biopsied