1,306 research outputs found
Finding the First Stars: The Hamburg/ESO Objective Prism Survey
We report on a search for extremely metal-poor ([Fe/H]<-3.0) turnoff stars in
the Hamburg/ESO objective prism survey (HES). Metal-poor stars are selected by
automatic spectral classification. Extensive simulations show that the
selection efficiency for turnoff stars of [Fe/H]25% at B<16.5. Since
the HES is more than 1 mag deeper than the HK survey of Beers et al. (1992),
the HES offers the possibility to efficiently increase the total number of
metal-poor stars by at least a factor of 4.Comment: To appear in: Proceedings of ESO/MPA conference "The First Stars". 2
pages, 1 figur
Directed evolution of an enantioselective Bacillus subtilis lipase
Chiral compounds are of steadily increasing importance to the chemical industry, in particular for the production of pharmaceuticals. Where do these compounds come from? Apart from natural resources, two synthetic strategies are available: asymmetric chemical catalysis using transition metal catalysts and biocatalysis using enzymes. In the latter case, screening programs have identified a number of enzymes. However, their enantioselectivity is often not high enough for a desired reaction. This problem can be solved by applying directed evolution to create enantioselective enzymes as shown here for a lipase from Bacillus subtilis. The reaction studied was the asymmetric hydrolysis of meso-1,4-diacetoxy-2-cyclopentene with the formation of chiral alcohols which were detected by electrospray ionization mass spectrometry. Iterative cycles of random mutagenesis and screening allowed the identification of several variants with improved enantioselectivities. In parallel, we have started to use X-ray structural data to simulate the Bacillus subtilis lipase A-catalyzed substrate hydrolysis by using quantum mechanical and molecular mechanical calculations. This combined approach should finally enable us to devise more efficient strategies for the directed evolution of enantioselective enzymes
Measurement of the electric dipole moments for transitions to rubidium Rydberg states via Autler-Townes splitting
We present the direct measurements of electric-dipole moments for
transitions with for Rubidium atoms. The
measurements were performed in an ultracold sample via observation of the
Autler-Townes splitting in a three-level ladder scheme, commonly used for
2-photon excitation of Rydberg states. To the best of our knowledge, this is
the first systematic measurement of the electric dipole moments for transitions
from low excited states of rubidium to Rydberg states. Due to its simplicity
and versatility, this method can be easily extended to other transitions and
other atomic species with little constraints. Good agreement of the
experimental results with theory proves the reliability of the measurement
method.Comment: 12 pages, 6 figures; figure 6 replaced with correct versio
A planar multipole ion trap
We report on the realisation of a chip-based multipole ion trap manufactured
using micro-electromechanical systems (MEMS) technology. It provides ion
confinement in an almost field-free volume between two planes of radiofrequency
electrodes, deposited on glass substrates, which allows for optical access to
the trap. An analytical model of the effective trapping potential is presented
and compared with numerical calculations. Stable trapping of argon ions is
achieved and a lifetime of 16s is measured. Electrostatic charging of the chip
surfaces is studied and found to agree with a numerical estimate
Observation of coherent many-body Rabi oscillations
A two-level quantum system coherently driven by a resonant electromagnetic
field oscillates sinusoidally between the two levels at frequency
which is proportional to the field amplitude [1]. This phenomenon, known as the
Rabi oscillation, has been at the heart of atomic, molecular and optical
physics since the seminal work of its namesake and coauthors [2]. Notably, Rabi
oscillations in isolated single atoms or dilute gases form the basis for
metrological applications such as atomic clocks and precision measurements of
physical constants [3]. Both inhomogeneous distribution of coupling strength to
the field and interactions between individual atoms reduce the visibility of
the oscillation and may even suppress it completely. A remarkable
transformation takes place in the limit where only a single excitation can be
present in the sample due to either initial conditions or atomic interactions:
there arises a collective, many-body Rabi oscillation at a frequency
involving all N >> 1 atoms in the sample [4]. This is true even
for inhomogeneous atom-field coupling distributions, where single-atom Rabi
oscillations may be invisible. When one of the two levels is a strongly
interacting Rydberg level, many-body Rabi oscillations emerge as a consequence
of the Rydberg excitation blockade. Lukin and coauthors outlined an approach to
quantum information processing based on this effect [5]. Here we report initial
observations of coherent many-body Rabi oscillations between the ground level
and a Rydberg level using several hundred cold rubidium atoms. The strongly
pronounced oscillations indicate a nearly complete excitation blockade of the
entire mesoscopic ensemble by a single excited atom. The results pave the way
towards quantum computation and simulation using ensembles of atoms
Observation of mesoscopic crystalline structures in a two-dimensional Rydberg gas
The ability to control and tune interactions in ultracold atomic gases has
paved the way towards the realization of new phases of matter. Whereas
experiments have so far achieved a high degree of control over short-ranged
interactions, the realization of long-range interactions would open up a whole
new realm of many-body physics and has become a central focus of research.
Rydberg atoms are very well-suited to achieve this goal, as the van der Waals
forces between them are many orders of magnitude larger than for ground state
atoms. Consequently, the mere laser excitation of ultracold gases can cause
strongly correlated many-body states to emerge directly when atoms are
transferred to Rydberg states. A key example are quantum crystals, composed of
coherent superpositions of different spatially ordered configurations of
collective excitations. Here we report on the direct measurement of strong
correlations in a laser excited two-dimensional atomic Mott insulator using
high-resolution, in-situ Rydberg atom imaging. The observations reveal the
emergence of spatially ordered excitation patterns in the high-density
components of the prepared many-body state. They have random orientation, but
well defined geometry, forming mesoscopic crystals of collective excitations
delocalised throughout the gas. Our experiment demonstrates the potential of
Rydberg gases to realise exotic phases of matter, thereby laying the basis for
quantum simulations of long-range interacting quantum magnets.Comment: 10 pages, 7 figure
Characterization of copper nanoparticles synthesized by a novel microbiological method
The Substrate-Bound Crystal Structure of a Baeyer–Villiger Monooxygenase Exhibits a Criegee-like Conformation
The Baeyer\u2013Villiger monooxygenases (BVMOs) are a family of bacterial flavoproteins that catalyze the synthetically useful Baeyer\u2013Villiger oxidation reaction. This involves the conversion of ketones into esters or cyclic ketones into lactones by introducing an oxygen atom adjacent to the carbonyl group. The BVMOs offer exquisite regio- and enantiospecificity while acting on a wide range of substrates. They use only NADPH and oxygen as cosubstrates, and produce only NADP+ and water as byproducts, making them environmentally attractive for industrial purposes. Here, we report the first crystal structure of a BVMO, cyclohexanone monooxygenase (CHMO) from Rhodococcus sp. HI-31 in complex with its substrate, cyclohexanone, as well as NADP+ and FAD, to 2.4 \uc5 resolution. This structure shows a drastic rotation of the NADP+ cofactor in comparison to previously reported NADP+-bound structures, as the nicotinamide moiety is no longer positioned above the flavin ring. Instead, the substrate, cyclohexanone, is found at this location, in an appropriate position for the formation of the Criegee intermediate. The rotation of NADP+ permits the substrate to gain access to the reactive flavin peroxyanion intermediate while preventing it from diffusing out of the active site. The structure thus reveals the conformation of the enzyme during the key catalytic step. CHMO is proposed to undergo a series of conformational changes to gradually move the substrate from the solvent, via binding in a solvent excluded pocket that dictates the enzyme\u2019s chemospecificity, to a location above the flavin\u2013peroxide adduct where catalysis occurs.Peer reviewed: YesNRC publication: Ye
γ-Aminobutyric Acid (GABA) Is an Autocrine Excitatory Transmitter in Human Pancreatic β-Cells
OBJECTIVE: Paracrine signaling via gamma-aminobutyric acid (GABA) and GABA(A) receptors (GABA(A)Rs) has been documented in rodent islets. Here we have studied the importance of GABAergic signaling in human pancreatic islets. RESEARCH DESIGN AND METHODS: Expression of GABA(A)Rs in islet cells was investigated by quantitative PCR, immunohistochemistry, and patch-clamp experiments. Hormone release was measured from intact islets. GABA release was monitored by whole-cell patch-clamp measurements after adenoviral expression of alpha(1)beta(1) GABA(A)R subunits. The subcellular localization of GABA was explored by electron microscopy. The effects of GABA on electrical activity were determined by perforated patch whole-cell recordings. RESULTS: PCR analysis detected relatively high levels of the mRNAs encoding GABA(A)R alpha(2), beta(3,) gamma(2), and pi subunits in human islets. Patch-clamp experiments revealed expression of GABA(A)R Cl(-) channels in 52% of beta-cells (current density 9 pA/pF), 91% of delta-cells (current density 148 pA/pF), and 6% of alpha-cells (current density 2 pA/pF). Expression of GABA(A)R subunits in islet cells was confirmed by immunohistochemistry. beta-Cells secreted GABA both by glucose-dependent exocytosis of insulin-containing granules and by a glucose-independent mechanism. The GABA(A)R antagonist SR95531 inhibited insulin secretion elicited by 6 mmol/l glucose. Application of GABA depolarized beta-cells and stimulated action potential firing in beta-cells exposed to glucose. CONCLUSIONS: Signaling via GABA and GABA(A)R constitutes an autocrine positive feedback loop in human beta-cells. The presence of GABA(A)R in non-beta-cells suggests that GABA may also be involved in the regulation of somatostatin and glucagon secretion
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