Novel membrane sensor for determination of lamotrigine in pharmaceuticals and urine

Lamotrigine (LMT), chemically known as [6-(2,3-dichlorophenyl)-1,2,4-triazine-3,5-diamine], is a broad spectrum antiepileptic drug, used as monotherapy and as an adjunct with other antiepileptic drugs for treatment of partial and generalized toxic-clonic seizures. It is used to treat neurological lesions and as a tranquilizer. A selective electrochemical membrane sensor has been developed and validated for determination of LMT. The membrane constructed using LMT and molybdophosphoric acid in THF and PVC is applicable for the detection of 5 × 10-4 to 9 × 10-3 M LMT in the pH range between 4.6 and 5.8 with the Nernstian slope of 57.14±1 mV/decade. The regression coefficient value of 0.9932 showed a good linear correlation between the concentrations of LMT and measured cell potentials. The limits of detection (LOD) and quantification (LOQ) values for the fabricated sensor were 1.3 × 10-5 and 4 × 10-5 M LMT, respectively. Various experimental conditions were optimized to reach the effective performance characteristics of the sensor. The effect of various cations, anions and organic species on the performance of sensor was studied by following standard-addition procedure. The results revealed no such variations due to presence of foreign ions or species. The fabricated sensor was subjected to validation to check accuracy, precision, robustness and ruggedness. The mean accuracy for determination of LMT was found to be 99.16%. The developed sensor was successfully used to determine LMT in tablets and in spiked human urine

Impact of Innovations in Value Chain on Sorghum Farmers

The paper has studied the implementation of millets value chain pilot project under the NAIP to create demand for sorghum through diversification in farm production, procurement, ultimately aiming at economical benefits to both farmers and other stakeholders. This 4.5 years novel initiative is still in the mid-way. The study has reported that technological backstopping of sorghum cultivation with end-product specific improved cultivars has realized 51 per cent rise in incremental net income of Rs 16098/ha for the participating farmers (58 per cent rise in kharif and 44 per cent in rabi farmers). The inconvenience in the preparation of sorghum foods which has been the important reason for the decrease in its consumption, has been eliminated through development of convenient and ready-to-eat/cook foods. The factorization of the investment expenditure made per farmer (one acre of sorghum per participating farmer) has been worked out to be Rs 356 (equivalent of Rs 890/ha) in a season resulting in 51 per cent rise in incremental net income over baseline which is slightly higher in kharif sorghum than in rabi sorghum. The output pricing in kharif has helped in attaining these benefits. However, the benefit-cost ratio has worked out in favour of rabi sorghum farmers, i.e. 1:7.5 vis-à-vis kharif farmers BC ratio of 1: 4.2. The study has observed that linking up of the entire stakeholders through value addition throughout the value chain system would renew and uplift the diminishing sorghum area and production and its ultimate economical benefits to farmers and other stakeholders in the value chain.Agricultural and Food Policy,

Spectrophotometric Determination of Mycophenolate Mofetil as Its Charge-Transfer Complexes with Two π-Acceptors

Two simple, selective, and rapid spectrophotometric methods are described for the determination of mycophenolate mofetil (MPM) in pure form and in tablets. Both methods are based on charge-transfer complexation reaction of MPM with p-chloranilic acid (p-CA) or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) in dioxane-acetonitrile medium resulting in coloured product measurable at 520 nm (p-CA) or 580 nm (DDQ). Beer's law is obeyed over the concentration ranges of 40–400 and 12–120 μg mL−1 MPM for p-CA and DDQ, respectively, with correlation coefficients (r) of 0.9995 and 0.9947. The apparent molar absorptivity values are calculated to be 1.06 × 103 and 3.87 × 103 L mol−1 cm−1, respectively, and the corresponding Sandell's sensitivities are 0.4106 and 0.1119 μg cm−1. The limits of detection (LOD) and quantification (LOQ) are also reported for both methods. The described methods were successfully applied to the determination of MPM in tablets. Statistical comparison of the results with those of the reference method showed excellent agreement. No interference was observed from the common excipients present in tablets. Both methods were validated statistically for accuracy and precision. The accuracy and reliability of the methods were further ascertained by recovery studies via standard addition procedure

(E)-1-(3-Bromophenyl)-3-(3-fluorophenyl)prop-2- en-1-one

In the title compound, C15H10BrFO, the olefinic double bond adopts an E conformation. The mol­ecule is non-planar as seen by the dihedral angle of 48.92 (11)° between the bromo­phenyl and fluoro­phenyl rings. The carbonyl group is twisted from the plane of the bromo­phenyl ring and the olefinic double bond. The trans conformation of the C=C double bond in the central enone group is confirmed by the C—C=C—C torsion angle of −165.7 (2)°

(2E,20E)-1,10-(1,4-Phenylene)bis[3-(3-chlorophenyl)- prop-2-en-1-one]

The title bis-chalcone compound, C24H16Cl2O2, crystallizes with one halfmolecule in the asymmetric unit. The molecule has crystallographic inversion symmetry and lies about an inversion centre at the centroid of the central benzene ring. The olefinic double bonds adopt E configurations. The s-trans conformation of the central C—C bond of the enone group is confirmed by a C—C—C C torsion angle of �162.88 (17)�

UV/Visible spectroscopic quantification of veterinary anthelmintic drug oxfendazole in pharmaceuticals

Oxfendazole (OFA) is a broad spectrum benzimidazole anthelmintic medication used to treat veterinary diseases. Two new, simple, rapid, precise and accurate, and sensitive spectrophotometric methods have been developed and validated for the assay of OFA in its pure form and in its boluses. The first method (method A) is based on the measurement of green colored manganate obtained due to oxidation of OFA by permanganate in an alkaline medium at 610 nm. In the second method (Method B), the chromophoric activity of OFA is measured at 290 nm in the acetic acid–water (1:1) solvent system. OFA has been quantified over the linear ranges of 1.25–12.5 and 1.25–10 μg/mL in method A and method B, respectively, with apparent molar absorptivity values of 2.54 × 104 and 2.13 × 104 L·mol–1·cm–1. The limit of detection (LOD), limit of quantification (LOQ), and Sandell sensitivity values are 0.15 u 0.18 μg/mL, 0.46 and 0.56 μg/mL, and 0.0124 and 0.0148 μg/cm2. The developed methods were successfully applied to determine OFA in the bolus. No interference from adjuvants was observed

Highly sensitive spectrophotometric determination of olanzapine using cerium(IV) and iron(II) complexes of 1,10-phenanthroline and 2,2´x-bipyridyl

Two highly sensitive spectrophotometric methods have been developed for the determination of olanzapine (OLP) in pharmaceuticals using cerium(IV) and iron(II) complexes of 1,10-phenanthroline and 2,2´x-bipyridyl as reagents. The methods are based on the oxidation of OLP in acidic medium by a known excess of cerium(IV) followed by the determination of the unreacted oxidant by reacting with either ferroin and measuring the absorbance at 510 nm (method A) or iron(II)-2,2´x-bipyridyl complex and measuring the absorbance at 525 nm (method B). The amount of cerium(IV) reacted corresponds to the amount of OLP. In both the methods, the absorbance is found to increase linearly with OLP concentration as shown by the correlation coefficient (r) of 0.9980 and 0.9958 for method A and method B, respectively. The calibration graphs are linear over the concentration range of 0.2--2.0 μg/mL in both the methods. The calculated molar absorptivity values are 1.00 × 106 and 7.03 × 105 L/mol cm, for method A and method B. The LOD and LOQ values for method A are calculated to be 0.04 and 0.13 μg/mL and the values are 0.07 and 0.22 μg/mL for method B, respectively. The methods were validated as per the current ICH guidelines. Both the methods gave similar results in terms of accuracy and precision. The RSD was less than 3% and the accuracy, obtained from recovery experiments, was 98.76--101.4%. The methods developed were applied to the determination of OLP in tablets and results agreed well with the label claim

Titrimetric assay of hydroxyzine dihydrochloride in pharmaceuticals and formulations in non-aqueous medium

Hydroxyzine, a piperazine H1-receptor antagonists effective in generalized anxiety disorder. Two simple, rapid, reliable, precise and accurate and cost-effective non-aqueous titrimetric procedures have been developed for the determination of hydroxyzine dihydrochloride (HDH) in bulk drug and its pharmaceutical formulations. The methods are based on the titration of the drug in glacial acetic acid in the presence of mercuric acetate with acetous perchloric acid to the visual end point using crystal violet as indicator and to the potentiometric end point. The methods were applicable over the range of 2-20 mg HDH. The procedures were also applied for the determination of HDH in its dosage forms and the results were found to be in a good agreement with those obtained by the reference method. The precision results, expressed by intra-day and interday relative standard deviation values, were satisfactory (RSD = 1.28 ). The accuracy was satisfactory as well (RE = 1.33 ). Excipients used as additives in pharmaceutical formulations did not interfere in the proposed procedures as shown by the recovery study via standard addition technique with percentage recoveries in the range 97.75-101.5 with a standard deviation of = 1.52

Quantitation of pyrantel pamoate in pharmaceuticals using permanganate by visible spectrophotometry

Two simple, accurate and precise spectrophotometric methods are developed and validated for the assay of pyrantel pamoate (PP) in pharmaceuticals. The methods employ the oxidative property of potassium permanganate (KMnO4) in acidic and alkaline conditions. In the first method (method A), PP is converted into its free base, pyrantel (PR), and treated with known excess of KMnO4 in acidic condition followed by the measurement of unreacted KMnO4 at 550 nm. Method B is based on the registration of absorbance of green colored chromogen formed due to the reduction of KMnO4 by PP in alkaline condition. The methods obeyed Beer's law over a range of 1-20 μg/ml in inverse manner, and 0.75-15 μg/ml for method A and method B, respectively, with apparent molar absorptivity values of 1.05dot operator104 and 2.85dot operator104 ldot operatormol-1dot operatorcm-1. The optical parameters such as limits of detection (LOD), quantification (LOQ), and the Sandell sensitivity values are also reported. The accuracy and precision of the methods are assessed on intra- and inter-day basis. A recovery study by standard addition procedure is also carried out for further assurance of accuracy. The developed methods are successfully applied to determine PP in tablets. The results are more satisfactory as per current ICH guidelines