IRIS SEGMENTATION USING GA BASED CIRCULAR HOUGH TRANSFORM (GACHT

Iris recognition system consists of a sequence of stages, each stage perform specific functions on the captured image of the human iris. The first stage is automatic segmentation of iris from the eye image. In this paper, new proposed algorithm designed and implemented using Genetic based Hough Transform algorithm (GACHT) to implement the first stage of iris recognition system with the developed Genetic Algorithm. GACHT was used to detect the Iris and Pupil segments which in turn is a outer and inner circles, the accuracy of any Iris recognition system should be depend on the right region of the Iris, which is done in this paper using and evolutionary algorithm called GACHT algorith

Studying the Optimum Conditions of ‎Hygromycin B Production and Detect their ‎Toxicity

تم استخلاص ال hygromycin B بأستخدام خلات الاثيل, فُصل المحتوى العضوي عن المائي في راشح المزرعه البكتيرية السائلة, واعطى المحتوى المائي فقط فعالية حيوية بأستخدام تقنية الأنتشار في الحفر على سطح الأغر (Agar well diffusion technique) عند تركيز25ملغم/مل (كمستخلص خام) , اعطى هذا الطور فعالية حيوية ضد مجموعة من الاحياء المجهرية اشتملت على بكتريا واحدة موجبة لغرام (Staphylococcus aureus) وخمس سالبة لغرام Pseudomonas aeruginosa) , Proteus mirabilis,, Escherichia coli, Klebsiella pneumoniae , Salmonella typhi) و خميرة واحدة( (Saccharomyces  cerevisiae.        عند الكشف عن ال hygromycin B aminoglycoside   بأستخدام  كروموتوغرافيا الطبقه الرقيقه لتأكيد وجود المضاد الحيوي, تم الحصول على نفس معدل الجريان (Rf) 0.357 للـ hygromycin B القياسي .        اظهرت نتائج الظروف المثلى للوسط الزرعي البكتيري ان اعلى فعالية حيوية للـ hygromycin B  تم الحصول عليها عند الرقم الهيدروجيني 8  والحضن بحرارة 35 م° لمدة 10 ايام.           تمت دراسة التأثير السمي للـ hygromycin B على كبد الفئران المختبرية وأظهرت تغيرات طفيفه على انسجة كبد الفأر.Hygromycin B was extracted with ethyl acetate, which separates organic phase from aqueous phase in the broth culture filtrate, only the aqueous phase showed significant antimicrobial activity by using agar well diffusion technique. At a concentration of 25mg/ml (as crude extract), this phase excreted its activity against the test microorganisms which include; one G(+) bacteria (Staphylococcus aureus), five G(–) bacteria (Pseudomonas aeruginosa , Proteus mirabilis, Escherichia coli , Klebsiella pneumoniae, Salmonella typhi) and one yeast (Saccharomyces  cerevisiae).          After detecting the aminoglycoside hygromycin B by the Thin Layer Chromatography (TLC) method to ensure presence of the antibiotic, same flow rate (Rf) value (0.357) as that of the standard hygromycin B was obtained.          Results of the optimization conditions showed that the highest antimicrobial activity of hygromycin B was obtained at a medium pH of 8 and incubation temperature of 35°C for 10 days. When the toxicity of hygromycin B crude extract under such conditions was examined on mice liver, a mild effects were appeare

Developed Protocol for Key Exchange Based on Irreducible Polynomial

The Aim of this paper is to design a protocol for key exchanging to work on the available computers for different data security application. This paper proposed idea to modify the Diffie-Hellman key exchange by using truncated polynomial instead of discrete logarithm problem to overcome the problem of large prime numbers and non-full coverage of the finite set. The proposed method depends on the arithmetic polynomials. The Irreducible truncated polynomial mathematics is highly efficient and compatible with personal computers

Novel sheathless CE-MS interface as an original and powerful infusion platform for nanoESI study: from intact proteins to high molecular mass noncovalent complexes.:

Development of nano-electrospray (nanoESI) sources allowed to increase significantly the sensitivity which is often lacking when studying biological noncovalent assemblies. However, the flow rate used to infuse the sample into the mass spectrometer cannot be precisely controlled with nanoESI and the robustness of the system could represent an issue. In this study, we have used a sheathless capillary electrophoresis-mass spectrometry (CESI) prototype as a nanoESI infusion device. The hydrodynamic mobilization of the capillary content was characterized and the ability of the system to generate a stable electrospray under controlled flow rate conditions ranging from 4 up to 900 nL/min was demonstrated. The effect of the infusing flow rate on the detection of an intact model protein analyzed under native conditions was investigated. Results demonstrated a significant increase in sensitivity of 46-fold and a signal-to-noise ratio improvement of nearly 5-fold when using an infusing flow rate from 456.9 down to 13.7 nL/min. The CESI prototype was further used to detect successfully the β ring homodimer in its native conformation. Obtained results were compared with those achieved with conventional ESI. Intensity signals were increased by a factor of 5, while sample consumption decreased 80 times. β ring complexed with the P14 peptide was also studied. Finally, the CESI interface was used to observe the quaternary structure of native hemocyanins from Carcinus maenas crabs; this high molecular complex coexisting under various degrees of complexation and resulting in masses ranging from 445 kDa to 1.34 MD

Structural characterization of antibody drug conjugate by a combination of intact, middle-up and bottom-up techniques using sheathless capillary electrophoresis – Tandem mass spectrometry as nanoESI infusion platform and separation method

Antibody-drug conjugates (ADCs) represent a fast growing class of biotherapeutic products. Their production leads to a distribution of species exhibiting different number of conjugated drugs overlaying the inherent com-plexity resulting from the monoclonal antibody format, such as glycoforms. ADCs require an additional level of characterization compared to first generation of biotherapeutics obtained through multiple analytical tech-niques for complete structure assessment. We report the development of complementary approaches imple-menting sheathless capillary electrophoresis-mass spectrometry (sheathless CE-MS) to characterize the differ-ent aspects defining the structure of brentuximab vedotin. Native MS using sheathless CE-MS instrument as a nanoESI infusion platform enabled accurate mass measurements and estimation of the average drug to anti-body ratio alongside to drug load distribution. Middle-up analysis performed after limited IdeS proteolysis allowed to study independently the light chain, Fab and F(ab’)2 subunits incorporating 1, 0 to 4 and 0 to 8 pay-loads respectively. Finally, a CZE-ESI-MS/MS methodology was developed in order to be compatible with hy-drophobic drug composing ADCs. From a single injection, complete sequence coverage could be achieved. Using the same dataset, glycosylation and drug-loaded peptides could be simultaneously identified revealing robust information regarding their respective localization and abundance. Drug-loaded peptide fragmentation mass spectra study demonstrated drug specific fragments reinforcing identification confidence, undescribed so far. Results reveal the method ability to characterize ADCs primary structure in a comprehensive manner while reducing tremendously the number of experiments required. Data generated showed that sheathless CZE-ESI-MS/MS characteristics position the methodology developed as a relevant alternative for comprehensive multi-level characterization of these complex biomolecule

Relation of Asthma Control with Quality of Life among a Sample of Egyptian Asthmatic School Children

BACKGROUND: Asthma is considered a chronic health illness that not only resulted in physical symptoms but also emotional effects. It is; therefore, so important to assess the quality of life of asthmatic patients besides their level of disease control. AIM: To determine the correlation of asthma control with the health-related quality of life (HRQOL) of asthmatic children in Egypt. METHODS: One hundred and twenty-eight asthmatic Egyptian children were enrolled in the study. They were subjected to asthma severity grading, asthma control questionnaire (ACQ) and pediatric asthma quality of life questionnaire (PAQLQ). Studied cases were taken from 6 primary and preparatory schools, Giza governorate. RESULTS: The mean child control score was significantly higher in not well-controlled asthmatics compared to well-controlled asthmatics (p < 0.005). The not well controlled asthmatic children showed significantly lower activity limitation score, symptoms score, and overall asthmatic score compared to controlled asthmatic children (p < 0.05). The severity of asthma shows significant positive correlation with symptoms score, emotional function score and overall asthmatic score (p < 0.05). CONCLUSION: The quality of life for the asthmatic children is strongly correlated with the level of asthma control and severity