3 research outputs found
The effects of iron-containing superoxide dismutases on haemodynamic parameters in spontaneously hypertensive rats
The product of FeSOD activity is hydrogen peroxide (H2O2). Furthermore, FeSOD can modify the
chemical versatility of NO into its redox
-active forms
: nitrosonium cation (NO+) and nitroxyl anion (NO–).
All of these low molecular weight species are vasoactive and, in particular, NO– induces calcitonin gene -
related peptide (CGRP) synthesis (known to be the most potent relaxation
-promoting peptide).In this study the effects of bolus infusions of iron
-containing superoxide dismutase (FeSOD) and
of superoxide dismutase containing both iron and manganese (FeMnSOD) on the arterial blood pressure
(MAP), the arterial blood pressure (CO) and the total vascular resistance (TVR) in spontaneously
hypertensive (SH) rats were determined.
Bolus infusion of FeSOD induced a biphasic response in the MAP (an initial increase was
followed by a significant decrease). At the end of the experiment the MAP returned to its basal
value.
FeMnSOD (the enzymatically inactive form of FeSOD) had no effect on the MAP in these experiments.
Bolus infusions of FeSOD and of FeMnSOD had no effect either on the both the CO or on the TVR in
SH rats.
Our results indicate that arterial relaxation changes mediated by NO–
may be important for
regulation of blood pressure in SH rats
Angiotensin 2 type 1 receptor blockade different affects postishemic kidney injury in normotensive and hypertensive rats
Many studies demonstrated that angiotensin 2 type 1 receptor (AT1R) blockade accelerates renal recovery in post-ischaemic kidney but there are many controversies related to its net effect on kidney structure and function. During the past years, our research group was trying to define the pathophysiological significance of the renin-angiotensin system on post-ischemic acute renal failure (ARF) development in normotensive Wistar as well as hypertensive rats (SHR). This review mostly summarizes our experience in that field. Our previous studies in normotensive rats revealed that AT1R blockade, except slightly renal vascular resistance improvement, had no other obvious beneficial effects, and therefore implies angiotensin 2 (Ang-2) overexpression as non-dominant on kidney reperfusion injuries development. Similarly it was observed in Wistar rats with induced mild (L-NAME, 3 mg/kg b.w.) nitric oxide (NO) deficiency. Expectably, in strong induced (L-NAME, 10 mg/kg b.w.) NO deficiency associated with ARF, massive tubular injuries indicate harmful effects of AT1R blockade, implying strongly disturbed glomerular filtration and suggesting special precaution related to AT1R blockers usage. Opposite to previous, by our opinion, AT1R antagonism promises new advance in treatment of essentially hypertensive subjects who develop ARF. Increased glomerular filtration, diminished oxidative stress, and most importantly improved tubular structure in postishemic SHR treated with AT1R blocker losartan, implicate Ang-2 over production as potently agent in the kidney ischemic injury, partly trough generation of reactive oxygen species. These data contribute understanding the pathogenesis of this devastating illness in hypertensive surroundings