Lead accumulation and metallothionein induction in different tissues of mussels (M. galloprovincialis) and clams (C. chione) exposed to various Pb concentrations

Heavy metal pollution of coastal areas due to anthropogenic activity has become a global problem with serious environmental consequences. Various marine organisms have been employed as bioindicators for pollution, and expanding the scientific arsenal with such organisms is a continuing research objective. In this study, the effects of Pb pollution in seawater on two bivalves of different habitats were investigated. Mytilus galloprovincialis (a nearshore and intertidal rocky substrate inhabitant mussel) and Callista chione, (a sand-buried smooth clam), were exposed to a wide range of Pb concentrations in seawater for 20 days, followed by a 10-day depuration period, in a laboratory experiment. Gills, mantles and the remaining bodies of the two species were separated and the accumulated Pb was determined. The synthesis of Pb-induced proteins (metallothioneins and/or other proteins) was also investigated in the tissues of the two species by Ellman assays, Coomasie-stained and fluorimetric SDS-PAGE experiments. Our results show that both species exhibit a relatively high tolerance to even very high Pb pollution levels and they accumulate considerable amounts of Pb in their tissues. Zinc levels in the examined tissues are affected in most of the tested conditions. The 10 days depuration period of our experiments was not enough for the complete removal of Pb from the organisms. Biochemical analysis showed that low molecular weight, sulfhydryl-containing proteins (metallothioneins), as well as proteins with molecular weight of about 50 kDa are synthesized in a species-dependent and tissue dependent way, due to Pb accumulation

Geophysical and geochemical study of geropotamos aquifer in the north-central coast of crete

The geological setting of Geropotamos aquifer on the north-central coast of Crete, Greece, is considered complex, while the local tectonic regime of the study area is characterized by two sets of faults orientated NW-SE and NE-SW. Investigation of the aquifer using the Transient ElectroMagnetic method (TEM) has resulted initially in 1D models of geoelectric structures and a final 3D geoelectrical model was constructed, depicting the zones of salination of groundwater in the aquifer. Groundwater samples were analysed and the most important chemical parameters were determined to provide an independent dataset for comparison with the TEM results, while Groundwater Quality Maps were produced. TEM and geochemical data correspond and provide verification of the TEM approach. As a result, saline intrusion is likely to occur along fractures in a fault zone through bedrock, and this work emphasises the critical role of fracture pathways in salination problems of coastal aquifers

Assembly and function of the Photosystem II manganese stabilizing protein: lessons from its natively unfolded behavior

The Photosystem II (PS II) manganese stabilizing protein (MSP) possesses characteristics, including thermostability, ascribed to the natively unfolded class of proteins (Lydakis-Simantiris et al. (1999) Biochemistry 38: 404–414). A site-directed mutant of MSP, C28A, C51A, which lacks the -S–S- bridge, also binds to PS II at wild-type levels and reconstitutes oxygen evolution activity [Betts et al. (1996) Biochim Biophys Acta 1274: 135–142], although the mutant protein is even more disordered in solution. Both WT and C28A, C51A MSP aggregate upon heating, but an examination of the effects of protein concentration and pH on heat-induced aggregation showed that each MSP species exhibited greater resistance to aggregation at a pH near their p I (5.2) than do either bovine serum albumin (BSA) or carbonic anhydrase, which were used as model water soluble proteins. Increases in pH above the p I of the MSPs and BSA enhanced their aggregation resistance, a behavior which can be predicted from their charge (MSP) or a combination of charge and stabilization by -S–S- bonds (BSA). In the case of aggregation resistance by MSP, this is likely to be an important factor in its ability to avoid unproductive self-association reactions in favor of formation of the protein–protein interactions that lead to formation of the functional oxygen evolving complex.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43544/1/11120_2004_Article_7759.pd

Bioconcentration of Cd and Ni in various tissues of two marine bivalves living in different habitats and exposed to heavily polluted seawater

Two marine bivalves, Mytilus galloprovincialis and Callista chione, were exposed to various concentrations of cadmium and nickel (0.5, 1.0, 2.5 and 20 ppm), for 20 days, plus 10 days’ depuration period, in a laboratory experiment. Animals from each experimental condition were dissected and the bioaccumulation and distribution of Cd and Ni were determined in their gills, mantles and remaining bodies. The concentrations of Fe and Zn were also measured. Heavy metal tolerance, bioconcentration and distribution of heavy metals in tissues were considerably different in M. galloprovincialis and C. chione: (i) both animals were tolerant to Ni pollution, even at the highest concentration used; (ii) C. chione was more tolerant to Cd; (iii) M. galloprovincialis was a better Cd and Ni accumulator, with the exception of the highest Cd concentration tested, where C. chione accumulated more Cd; (iv) Fe and Zn levels were much more affected in M. galloprovincialis; (v) in general, accumulation and distribution of Ni and Cd in the tissues were metal-, species- and time of exposure- dependent; (vi) significant amounts of heavy metals remained in the tissues after 10 days’ depuration. Our results support a hypothesis for a two competing processes mechanism for metal accumulation and detoxification. © 2014, © 2014 Taylor & Francis

Experimental study of cadmium bioaccumulation in three Mediterranean marine bivalve species: Correlation with selected biomarkers

An ecotoxicological study is presented, in which three marine bivalve species (Mytilus galloprovincialis, Callista chione, and Venus verrucosa) living in different habitats were studied for Cd bioaccumulation, under laboratory conditions. The bivalves, originating from a relatively polluted marine area of Greece (Saronicos Gulf), were exposed to 0.5 mg Cd L-1 seawater (4.4 μmol Cd L-1 seawater) for 5, 10, 15, and 20 days. Control animals were kept in metal-free seawater as well. Three or four different parts of the organisms (gills, mantle, body, digestive system) were examined for the bioaccumulation of Cd, as well as the levels of three biomarkers (metallothioneins, acetylcholinesterase, lipid peroxidation). A depuration experiment was also carried out. During the experiment, the initial levels of Cd in the control animal tissues either decreased or remained constant and low. The organisms exhibited different behavior regarding Cd bioconcentration and biomarker responses as well as tissue distribution of Cd. After the depuration period, significant amounts of Cd remained in the organisms' tissues, much higher than the respective levels in control animals. © 2014 IUPAC & De Gruyter

Behavior and trends for Zn in Saronikos Gulf

The study of Zn in the water column of Saronikos Gulf in Greece during a two year period between 2008 and 2010 added data to the available time series of metal data for this marine area since 1985. The Saronikos Gulf, is directly influenced by the Athens metropolitan area. The operation of the Wastewater Treatment Plant of Athens situated on the small island of Psitalia, in 1995, was considered to be the turning point in the efforts of de-pollution of the gulf. Major sources of pollution for the gulf include also the port of Piraeus, with intensive navigation and shipping activities and the significant industrial activity occurring along the coast of Attica. This study of dissolved and particulate Zn gave results consistent with previous studies of the area such as the prevalence of the dissolved form of Zn and the most affected areas being the smaller most enclosed Gulf of Elefsina and Psitalia Island near the wastewater outfall. However, the concentrations of Zn were below toxic levels for marine aquatic organisms. Furthermore, a clear decrease in the Eastern part of the gulf was exhibited as well as a more subtle decrease in the gulf of Elefsina. The levels of Zn in Saronikos Gulf were found comparable to those of other Greek coastal areas

Comparative Study of the Accumulation of Ni in Different Tissues of Mussels and Soft Clams

Two bivalves, the mussel Mytilus galloprovincialis, and the soft clam Callista chione were exposed to various Ni concentrations for a total period of 20 days, and then a depuration period of 10 days followed. Nickel accumulation in gills, mantle and the remaining body of the animals was measured after 5, 10, 15 and 20 days of exposure to Ni-contaminated seawater. The two organisms showed different behavior regarding Ni accumulation in all three tissues examined. In all concentrations, Ni accumulation increased with time in all three tissues of Mytilus galloprovincialis with gills being the most effective accumulator per dry mass. On the other hand, Callista chione showed an initial response to Ni contamination the first 5 days, followed by stabilization or even a slight decrease of Ni accumulation

Novel quality control methods in conjunction with chemometrics (multivariate analysis) for detecting honey authenticity

The importance of honey has been recently upgraded because of its nutrient and therapeutic effect. The adulteration of honey increased exponentially in terms of both geographic and/or botanical origin. Therefore, the need has arisen for more effective quality control methods aiming at detecting adulteration. Various novel, fast, and accurate methods like AAS, HPLC, GC-MS, ES-MS, TLC, HPAED-PAD, NMR, FT-Raman, and NIR have enriched the arsenal of analytical chemist in this direction. However, apart from these novel methods, the application of multivariate analysis and, in particular PCA, CLA, and CA, proved to be extremely useful for grouping and detecting honey of various origins. Mineral and trace element analysis were repeatedly shown to be a very effective means for the classification purposes of honey of various origins (geographical and botanical)