Embryonic and post-embryonic utilization and subcellular localization of the nuclear receptor SpSHR2 in the sea urchin

SpSHR2 (Strongylocentrotus purpuratus steroid hormone receptor 2) is a nuclear receptor, encoded by a maternal RNA in the sea urchin embryo. These maternal SpSHR2 transcripts, which are present in all cells, persist until the blastula stage and then are rapidly turned over. A small fraction of the embryonic SpSHR2 protein is maternal, but the majority of this nuclear receptor in the embryo is the product of new synthesis, presumably from the maternal RNA after fertilization. In agreement with the mRNA distribution, the SpSHR2 protein is also detected in all embryonic cells. Contrary to the RNA though, the SpSHR2 protein persists throughout embryonic development to the pluteus stage, long after the mRNA is depleted. Following fertilization and as soon as the 2-cell stage, the cytoplasmic SpSHR2 protein enters rapidly into the embryonic nuclei where it appears in the form of speckles. During subsequent stages (from fourth cleavage onward), SpSHR2 resides in speckled form in both the nucleus and the cytoplasm of the embryonic cells. The cytoplasmic localization of SpSHR2 differs between polarized and non-polarized cells, maintaining an apical position in the ectoderm and endoderm versus a uniform distribution in mesenchyme cells. Following the end of embryonic development (pluteus stage), the SpSHR2 protein is depleted from all tissues. During the ensuing four weeks of larval development, the SpSHR2 is not detected in either the larval or the rudiment cells which will give rise to the adult. Just prior to metamorphosis, at about 35 days post-fertilization, the protein is detected again but in contrast to the uniform distribution in the early embryo, the larval SpSHR2 is specifically expressed in cells of the mouth epithelium and the epaulettes. In adult ovaries and testes, SpSHR2 is specifically detected in the myoepithelial cells surrounding the ovarioles and the testicular acini. Nuclear SpSHR2 in blastula extracts binds to the C1R hormone response element in the upstream promoter region of the CyIIIb actin gene indicating that the latter may be a target of this nuclear receptor in the sea urchin embryo

Influence of the passive region on Zero Field Steps for window Josephson junctions

We present a numerical and analytic study of the influence of the passive region on fluxon dynamics in a window junction. We examine the effect of the extension of the passive region and its electromagnetic characteristics, its surface inductance and capacitance. When the velocity in the passive region $v_{I}$ is equal to the Swihart velocity (1) a one dimensional model describes well the operation of the device. When $v_{I}$ is different from 1, the fluxon adapts its velocity to $v_{I}$. In both cases we give simple formulas for the position of the limiting voltage of the zero field steps. Large values of inductance and capacitance lead to different types of solutions which are analyzed.Comment: 12 pages, 13 figure

Critical currents in Josephson junctions with macroscopic defects

The critical currents in Josephson junctions of conventional superconductors with macroscopic defects are calculated for different defect critical current densities as a function of the magnetic field. We also study the evolution of the different modes with the defect position, at zero external field. We study the stability of the solutions and derive simple arguments, that could help the defect characterization. In most cases a reentrant behavior is seen, where both a maximum and a minimum current exist.Comment: 17 pages with 16 figures, submitted to Supercond. Sci. Techno

Nonequilibrium Electron Interactions in Metal Films

Ultrafast relaxation dynamics of an athermal electron distribution is investigated in silver films using a femtosecond pump-probe technique with 18 fs pulses in off-resonant conditions. The results yield evidence for an increase with time of the electron-gas energy loss rate to the lattice and of the free electron damping during the early stages of the electron-gas thermalization. These effects are attributed to transient alterations of the electron average scattering processes due to the athermal nature of the electron gas, in agreement with numerical simulations

Lineage-specific Gene Expression in the Sea Urchin Embryo

Within a few days of fertilization, the sea urchin embryo develops into a small differentiated organism consisting of about 1800 cells and capable of feeding, swimming, and the further ontogenic transformations required in the succeeding weeks of larval growth. A number of distinct cell lineages that are clearly specialized at the morphological and functional levels can be discerned in the advanced embryo, and many of these can be traced back to particular sets of early blastomeres. Classical cell lineage and experimental studies (Hörstadius 1939; for review, see Angerer and Davidson 1984) have shown that certain of these lineages appear to be specified, at least in part, in consequence of the maternal components inherited in those regions of egg cytoplasm occupied by their progenitor cells. Specification of others among the early cell lineages clearly depends on inductive interactions that occur between blastomeres during cleavage. For the molecular biologist, as for his predecessors, this rapidly developing and simply constructed embryo offers the advantages of experimental accessibility. Thus, in respect to direct molecular-level analyses of gene activity in the embryo, for both specific genes and overall transcript populations and their protein products, the sea urchin is at present the best known embryonic system (e.g., reviews of Hentschel and Birnstiel 1981; Davidson et al. 1982; Angerer and Davidson 1984)

Critical currents in Josephson junctions, with unconventional pairing symmetry: dx2−y2+isd_{x^2-y^2}+is versus dx2−y2+idxyd_{x^2-y^2}+id_{xy}

Phenomenological Ginzburg-Landau theory is used to calculate the possible spontaneous vortex states that may exist at corner junctions of $d_{x^2-y^2}+ix$-wave, (where $x=s$ or $x=d_{xy}$) and s-wave superconductors. We study the magnetic flux and the critical current modulation with the junction orientation angle $\theta$, the magnitude of the order parameter, and the magnetic field $H$. It is seen that the critical current $I_c$ versus the magnetic flux $\Phi$ relation is symmetric / asymmetric for $x=d_{xy}/s$ when the orientation is exactly such that the lobes of the dominant $d_{x^2-y^2}$-wave order parameter points towards the two junctions, which are at right angles for the corner junction. The conclusion is that a measurement of the $I_c(\Phi)$ relation may distinguish which symmetry ($d_{x^2-y^2}+is$ or $d_{x^2-y^2}+id_{xy}$) the order parameter has.Comment: 11 pages with 11 figures, Changed conten

Magnetic-interference patterns in Josephson junctions with d+is symmetry

The magnetic interference pattern and the spontaneous flux in unconventional Josephson junctions of superconductors with d+is symmetry are calculated for different reduced junction lengths and the relative factor of the d and s wave components. This is a time reversal broken symmetry state. We study the stability of the fractional vortex and antivortex which are spontaneously formed and examine their evolution as we change the length and the relative factor of d and s wave components. The asymmetry in the field modulated diffraction pattern exists for lengths as long as L=10\lambda_J.Comment: 8 pages, 6 eps files, submitted to PR

Stability analysis of static solutions in a Josephson junction

We present all the possible solutions of a Josephson junction with bias current and magnetic field with both inline and overlap geometry, and examine their stability. We follow the bifurcation of new solutions as we increase the junction length. The analytical results, in terms of elliptic functions in the case of inline geometry, are in agreement with the numerical calculations and explain the strong hysteretic phenomena typically seen in the calculation of the maximum tunneling current. This suggests a different experimental approach based on the use, instead of the external magnetic field the modulus of the elliptic function or the related quantity the total magnetic flux to avoid hysteretic behavior and unfold the overlapping $I_{max}(H)$ curves.Comment: 36 pages with 17 figure

Solitons in anharmonic chains with ultra-long-range interatomic interactions

We study the influence of long-range interatomic interactions on the properties of supersonic pulse solitons in anharmonic chains. We show that in the case of ultra-long-range (e.g., screened Coulomb) interactions three different types of pulse solitons coexist in a certain velocity interval: one type is unstable but the two others are stable. The high-energy stable soliton is broad and can be described in the quasicontinuum approximation. But the low-energy stable soliton consists of two components, short-range and long-range ones, and can be considered as a bound state of these components.Comment: 4 pages (LaTeX), 5 figures (Postscript); submitted to Phys. Rev.