DNA plasmidial de isolados patogênicos de Xanthomonas campestris pv. viticola.

Poucos são os estudos a respeito da presença de plasmídeos neste patógeno. Nos animais, bactérias patogênicas são conhecidas por albergar plasmídeos com diferentes funções entre elas a resistência, metabolismo de produtos tóxicos e transferência de material genético (Sherley et al., 2004). Nosso estudo teve por objetivo caracterizar o conteúdo de plasmídeos de isolados de X. campestris pv viticola isoladas de casos clínicos

Efeito de doses e periodos de imersao de paclobutrazol na cultura do alho.

O trabalho foi conduzido no campo Experimental do Setor de Olericultura da UFLA, Lavras,MG. Utilizou-se o delineamento experimental de blocos ao acaso em esquema fatorial 4 x 5, compreendendo quatro doses de paclobutrazol e cinco periodos de imersao. Observou-se que com o incremento de doses de pachobutrazol uma reducao na altura da planta e no numero de folhas aos 60 e 90 dias apos o plantio, assim como um aumento no peso medio de bulbos e bulbos graudos. Para bulbos medios e pequenos o pachobutrazol proporcionou um efeito quadratico com ponto de minima producao. Com relacao a producao total e comercial, percentagem de bulbos pseudoperfilhados e numero de bulbilhos por bulbo nao observou-se diferencas significativas

Eficiencia de fungicidas no controle da queima das folhas (Alternaria dauci (Kuhn) Groves & Skolko) na cultura da cenoura (Daucus carota L.)

Com o objetivo de avaliar a eficiencia de diversos fungicidas no controle da queima das folhas de cenoura, instalou-se em Lavras-MG, um ensaio no delineamento de blocos ao acaso com 10 tratamentos (procymidone a 500 e 750 g/ha; triflumizole a 300 e 450 g/ha; tiofanato metilico + chlorothlonil a 500 + 1250 g/ha; chlorothalonil a 1500 g/ha; iprodionea 750 g/ha; oxicloreto de cobre + chlorothalonil a 600 + 500 g/ha; chlorothalonil + enxofre a 1125 + 1300 g/ha do ingrediente ativo e testemunha) e 4 repeticoes. Os fungicidas iprodione e procymidone foram os mais eficientes em reduzir significativamente a incidencia de queima das folhas e incrementar a producao e peso medio de raizes comerciais

Differences in disability and nutritional status among older Brazilian and English adults: the Brazilian Longitudinal Study of Aging (ELSI-Brazil) and English Longitudinal Study of Aging (ELSA) cohorts

Background: Brazil and England are 2 countries at different stages in their demographic, epidemiological, and nutritional transitions and with distinct socioeconomic and politic contexts, but with similar universal health systems. We aimed to examine disability and its association with objective anthropometric indicators of nutritional status, including BMI, waist circumference, and waist-to-height ratio, comparing older Brazilian and English adults. / Methods: We used cross-sectional data from 2 nationally representative aging studies. For Brazil, we included 9412 participants who participated in the baseline (2015–2016) of the Brazilian Longitudinal Study of Aging (ELSI-Brazil). The English data were from 8024 participants of the wave 6 (2012–2013) of the English Longitudinal Study of Aging (ELSA). Disability was defined as difficulty to perform at least 1 activity of daily living. We used logistic regression models to examine the association between anthropometric indicators and disability, adjusted for sociodemographic and health-related characteristics, considering the interaction term between each anthropometric indicator and country. / Results: All health-related characteristics were worse in Brazil than England, although the prevalence of disability was similar among Brazilian (17.85%) and English (16.27%) older adults. Fully adjusted models showed statistically significant interaction terms between country and anthropometric indicators. The strength of the associations in Brazil was weaker compared with England. All anthropometric indicators were positively associated with disability: elevated BMI, in Brazil (OR: 1.27; 95% CI: 1.06, 1.51) and in England (OR: 1.80; 95% CI: 1.51, 2.14); elevated waist circumference, in Brazil (OR: 1.21; 95% CI: 1.02, 1.44) and in England (OR: 1.90; 95% CI: 1.51, 2.37); and elevated waist-to-height ratio, in Brazil (OR: 1.20; 95% CI: 0.96, 1.52) and in England (OR: 1.83; 95% CI: 1.37, 2.44). / Conclusions: Elevated BMI and waist circumference increased the odds of disability in both populations. However, these associations were stronger in England than in Brazil

Influence of ClearT and ClearT2 Agitation Conditions in the Fluorescence Imaging of 3D Spheroids

3D tumor spheroids have arisen in the last years as potent tools for the in vitro screening of novel anticancer therapeutics. Nevertheless, to increase the reproducibility and predictability of the data originated from the spheroids it is still necessary to develop or optimize the techniques used for spheroids' physical and biomolecular characterization. Fluorescence microscopy, such as confocal laser scanning microscopy (CLSM), is a tool commonly used by researchers to characterize spheroids structure and the antitumoral effect of novel therapeutics. However, its application in spheroids' analysis is hindered by the limited light penetration in thick samples. For this purpose, optical clearing solutions have been explored to increase the spheroids' transparency by reducing the light scattering. In this study, the influence of agitation conditions (i.e., static, horizontal agitation, and rotatory agitation) on the ClearT and ClearT2 methods' clearing efficacy and tumor spheroids' imaging by CLSM was characterized. The obtained results demonstrate that the ClearT method results in the improved imaging of the spheroids interior, whereas the ClearT2 resulted in an increased propidium iodide mean fluorescence intensity as well as a higher signal depth in the Z-axis. Additionally, for both methods, the best clearing results were obtained for the spheroids treated under the rotatory agitation. In general, this work provides new insights on the ClearT and ClearT2 clearing methodologies and their utilization for improving the reproducibility of the data obtained through the CLSM, such as the analysis of the cell death in response to therapeutics administration.info:eu-repo/semantics/publishedVersio

Caracterização molecular da dinâmica de grupos bacterianos durante a transição entre área nativa e cultivada de um Argissolo da Caatinga do Sertão pernambucano.

Buscou-se conhecer a estrutura e as mudanças ocorridas na comunidade microbiana edáfica de uma área de argissolo do Vale do São Francisco que passou de área nativa à cultivada

Serological, parasitological and molecular assessment of Babesia bovis and Babesia bigemina in cattle from State Maranhão.

The aim of the present study was to determine the prevalence of Babesia bovis and Babesia bigemina in dairy cattle from São Luis Island in the state of Maranhão, Brazil. A total of 281 blood samples were collected. In total, 275 (97.9%) animals were B. bovis-reactive and B. bigemina-reactive in the Enzyme-Linked Immunosorbent Assay (ELISA). The microscopy examination detected 22 (7.8%) animals that were positive for Babesia sp. and the Polymerase Chain Reaction (PCR) analysis showed that 91 animals (32.38%) and 23 animals (8.18%) were positive for B. bovis and B. bigemina, respectively, while 17 animals (6.04%) were co-infected. There is a high level of transmission of these protozoa in Maranhão, and the animals were naturally exposed. Therefore, it is possible to characterize the island as enzootic stability for babesiosis, indicat-ing a risk of financial losses when susceptible animals are introduced from areas of enzootic instability or free regions of B. bovis and B. bigemina.Título em português: Avaliação sorológica, parasitológica e molecular de Babesia bovis e Babesia bigemina no gado do Estado do Maranhão