APOE genotype and entorhinal cortex volume in non-demented community-dwelling adults in midlife and early old age

Copyright © 2012 IOS PressThis article has been made available through the Brunel Open Access Publishing Fund.The apolipoprotein E (APOE) ε4 allele is a risk factor for the neuropathological decline accompanying Alzheimer's disease (AD) while, conversely, the ε2 allele offers protection. One of the brain structures exhibiting the earliest changes associated with the disease is the entorhinal cortex. We therefore investigated the volumes of the entorhinal cortex and other structures in the medial temporal lobe including the parahippocampal gyrus, temporal pole, and inferior, middle, and superior temporal cortices, in relation to APOE genotype. Our main objectives were to determine if (a) volumes systematically varied according to allele in a stepwise fashion, ε2 > ε3 > ε4, and (b) associations varied according to age. We investigate this association in 627 non-demented community-dwelling adults in middle age (44 to 48 years; n = 314) and older age (64 to 68 years; n = 313) who underwent structural MRI scans. We found no evidence of APOE-related variation in brain volumes in the age groups examined. We conclude that if a ε2 > ε3 > ε4 pattern in brain volumes does emerge in non-demented adults living in the community in old age, it is not until after the age of 68 years.This study was funded by the UK Leverhulme Trust, the British Academy, the NHMRC Research Fellowship No. 471501, the NHMRC Research Fellowship No.#1002560, the National Health and Medical Research Council of Australia Unit Grant No. 973302, Program Grant No. 179805, Project grant No. 157125; Program grant no. 350833, and the National Computational Infrastructure. This article is made available through the Brunel Open Access Publishing Fund

Geographical variation in certification rates of blindness and sight impairment in England, 2008-2009

To examine and interpret the variation in the incidence of blindness and sight impairment in England by PCT, as reported by the Certificate of Vision Impairment (CVI). Design: Analysis of national certification data. Setting: All Primary Care Trusts, England. Participants: 23 773 CVI certifications issued from 2008 to 2009. Main Outcome measures: Crude and Age standardised rates of CVI data for blindness and sight loss by PCT. Methods: The crude and age standardised CVI rates per 100 000 were calculated with Spearman's rank correlation used to assess whether there was any evidence of association between CVI rates with Index of Multiple Deprivation (IMD) and the Programme Spend for Vision. Results: There was high-level variation, almost 11-fold (coefficient of variation 38%) in standardised CVI blindness and sight impairment annual certification rates across PCTs. The mean rate was 43.7 and the SD 16.7. We found little evidence of an association between the rate of blindness and sight impairment with either the IMD or Programme Spend on Vision. Conclusions: The wide geographical variation we found raises questions about the quality of the data and whether there is genuine unmet need for prevention of sight loss. It is a concern for public health practitioners who will be interpreting these data locally and nationally as the CVI data will form the basis of the public health indicator ‘preventable sight loss’. Poor-quality data and inadequate interpretation will only create confusion if not addressed adequately from the outset. There is an urgent need to address the shortcomings of the current data collection system and to educate all public health practitioners

Helicity amplitudes and crossing relations for antiproton proton reactions

Antiproton proton annihilation reactions allow unique access to the moduli and phases of nucleon electromagnetic form factors in the time like region. We present the helicity amplitudes for the unequal mass single photon reaction $p \bar{p}\to l^+ l^-$ in the s channel including the lepton mass. The relative signs of these amplitudes are determined using simple invariance properties. Helicity amplitudes for the annihilation reaction $p \bar{p} \to N \bar{N}$ are also given, where $N$ is any spinor particle with structure. Crossing relations between the $e p \to e p$ scattering and the $p \bar{p}\to l^+ l^-$ annihilation channels are discussed and the crossing matrix for the helicity amplitudes is given. This matrix may be used to verify known expressions for the space like helicity amplitudes due to one photon exchange.Comment: 15 pages, 5 figures, submitted to EPJ

Laser induced magnetization switching in films with perpendicular anisotropy: a comparison between measurements and a multi-macrospin model

Thermally-assisted ultra-fast magnetization reversal in a DC magnetic field for magnetic multilayer thin films with perpendicular anisotropy has been investigated in the time domain using femtosecond laser heating. The experiment is set-up as an optically pumped stroboscopic Time Resolved Magneto-Optical Kerr Effect magnetometer. It is observed that a modest laser fluence of about 0.3 mJ/square-cm induces switching of the magnetization in an applied field much less than the DC coercivity (0.8 T) on the sub-nanosecond time-scale. This switching was thermally-assisted by the energy from the femtosecond pump-pulse. The experimental results are compared with a model based on the Landau Lifschitz Bloch equation. The comparison supports a description of the reversal process as an ultra-fast demagnetization and partial recovery followed by slower thermally activated switching due to the spin system remaining at an elevated temperature after the heating pulse.Comment: 8 pages, 10 figures, to be submitted to PR

Comparison of bacterial biofilm communities using barcoded pyrosequencing and analysis to determine origin of biofilm fouling of reverse osmosis membranes in a full scale desalination system

Biofouling is the single most important issue in reverse osmosis sea water desalination worldwide (Ridgway et al., 1999) and may account for up to 50% of energy use. Which species are responsible and their origin is unclear. With the advent of next generation sequencing, species diversity and transience can be examined at orders of magnitude greater detail than was previously possible. We found many similarities in bacterial families across source water, prefiltration units and membranes in this study and in the few other studies available, despite disparate locations and seasons. Key groups included members of the Bacteroidetes (e. Flavobacteriaceae), Planctomycetes, Alphaproteobacteria (eg. Rhodobacteraceae, Sphingomonadales), Betaproteobacteria (eg. Burkholderia) and Gammaproteobacteria (eg. Oceanospirillales, Xanthomonadaceae). Despite similarities in families, the predominant fouling species on reverse osmosis (RO) membranes appear to differ between studies. This seems likely to reflect a common origin (seawater) but subsequent adaptation or selective pressures in different niches, particularly on RO membranes under high pressure and salt concentration. We can now select environmental isolates from our culture collection representing key bacterial groups responsible for biofouling in seawater systems. This will enable more accurate evaluation of the effectiveness of anti-fouling strategies

Ophthalmic statistics note 8: missing data - exploring the unknown

Medical research is conducted to answer uncertainties and to identify effective treatments for patients. Different questions are best addressed by different types of study design—but the randomised, controlled clinical trial is typically viewed as the gold standard, providing a very high level of evidence, when examining efficacy. While clinical trial methodology has advanced considerably with clear guidance provided as to how to avoid sources of bias, even the most robustly designed study can succumb to missing data. In this statistics note, we discuss strategies for dealing with missing data but what we hope emerges is a very clear message that there is no ideal solution to missing data and prevention is the best strategy