Biohemijske promene u krvnom serumu pasa tretiranih fenobarbitonom

Despite being described as the safest antiepileptic drug of first choice the presented literature data are much varied as far as dog blood serum biochemical parameters are considered. The aim of this study was to investigate the effect of phenobarbitone at different per os doses on the values of selected blood serum biochemical parameters in dogs during both short and long term application. The study was conducted on 30 dogs of different races, both sexes, ranging from 2 to 8 years of age. A total of 15 healthy and 15 dogs suffering from idiophatic epilepsy were observed. During the short term per os application of phenobarbitone (given at 3 week intervals) to the healthy population in varied doses a statistically significant increase in ALT and AP was recorded. Application of 16 mg/kg/day of phenobarbitone to the healthy population during 14 days resulted in a significant increase of ALT and AP. This increase was dependant on the duration of the treatment. During chronic application of phenobarbitone to dogs suffering from idiopathic epilepsy a significant increase in values of AP and ALT depending on the given dose was recorded. In two of the studied epileptic dogs treated with high therapeutic doses of phenobarbitone clinical signs of hepatotoxicity were recorded. Hepatotoxicity resulted in decreased albumin and total protein concentrations, as well as increased blood serum total bilirubin, AST, ALP and AP. The obtained results indicate that a long term application of phenobarbitone at high therapeutic doses can cause hepatotoxicity. However, there was no relationship between phenobarbitone dosage and duration of therapy and blood glucose, urea, creatinine, total proteins, albumins, total bilirubin, triglycerides and cholesterol.Iako označen kao antiepileptik prvog izbora i veoma bezbedan lek, u literaturi su prezentovani različiti podaci o uticaju fenobarbitona na vrednosti biohemijskih parametara krvnog seruma kod pasa. S obzirom na to, cilj ovog istraživanja bio je da se ispita uticaj fenobarbitona pri različitim per os dozama na vrednosti biohemijskih parametara krvnog seruma kod pasa, tokom kratkotrajne i dugotrajne aplikacije. Istraživanje je sprovedeno na 30 pasa različitih rasa, oba pola, starosne kategorije od dve do osam godina, od kojih su 15 bili zdravi psi a 15 psi oboleli od idiopatske epilepsije. Tokom kratkotrajne per os aplikacije fenobarbitona (intervali od po tri nedelje) zdravoj populaciji pasa pri različitim per os dozama, u krvnom serumu je registrovano značajno povećanje aktivnosti ALT i vrlo značajno povećanje aktivnosti AP u zavisnosti od doze leka. Aplikacija fenobarbitona u dozi od 16 mg/kg/dnevno tokom 14 nedelja zdravoj populaciji pasa ukazala je, na vrlo značajno povećanje aktivnosti ALT i AP u zavisnosti od dužine trajanja aplikacije. Tokom hronične aplikacije fenobarbitona psima obolelim od idiopatske epilepsije, ustanovljeno je statistički vrlo značajno povećanje aktivnosti AP i ALT u krvnom serumu u zavisnosti od doze leka. Kod dve jedinke u bolesnoj grupi pasa pri visokim terapeutskim dozama fenobarbitona registrovana je hepatotoksičnost, što je dovelo do statistički značajnog smanjenja koncentracije albumina i ukupnih proteina, statistički značajnog povećanja koncentracije ukupnog bilirubina, statistički značajnog povećanja aktivnosti AST i statistički vrlo značajnog povećanja aktivnosti ALT i AP u krvnom serumu Dobijeni rezultati ukazuju da dugotrajna aplikacija fenobarbitona, pri visokim terapeutskim dozama može izazvati hepatotoksičnost. Nije ustanovljeno postojanje veze između koncentracije glukoze, uree, kreatinina, ukupnih proteina, albumina, ukupnog bilirubina, triglicerida i holesterola u krvnom serumu pasa sa per os dozom fenobarbitona, niti sa dužinom trajanja terapije

Anatomical characteristics of two Ornithogalum L. (Hyacinthaceae) taxa from Serbia and Hungary and their taxonomic implication

Anatomical characters of two morphologically similar Ornithogalum taxa, O. umbellatum and O. divergens, were investigated. An analysis of leaf and scapus cross-sections was performed on plants from ten populations from Serbia and Hungary, using light microscopy. The aim of this research was to give data about the qualitative and quantitative anatomical characteristics of these taxa, in order to evaluate their taxonomic significance and single out distinctive anatomical features, as well as to contribute to the knowledge of the genus Ornithogalum in the studied region. On the basis of the variability of anatomical characters, similar populations formed two clusters, joining the plants previously determined as O. divergens and O. umbellatum. The two taxa significantly differed for most of the quantitative leaf and scapus characters. Since only quantitative differences were recorded in this research, anatomical characters could not be solely used to separate these two taxa. However, the results of anatomical investigations are consistent with the results of previous morphological and genetic analyses; therefore anatomical parameters could be useful as additional taxonomic characters

Životna proizvodnja visokomlečnih krava

Lifetime milk production is a key success factor in fulfilling the production potential of high-yielding cows. Lifetime milk production traits are pronouncedly variable. The life expectancy and the length of productive life of dairy cows are repeatedly limiting factors for improving lifetime milk production. Lifetime milk production is greatly depended on age at first calving and the number of lactations during productive life. Previous researches have implied there are real chances for improving the lifetime milk production of high-yielding cows. The goal of this research was to investigate the significance of key systematic factors on the lifetime production of high-yielding Black-and-White cows. The animals included in the sample had different share of Holstein genes. The researchers determined systematic factors that caused some significant phenotypic variations of the investigated trait. The average lifetime milk production was 25,002.66±7,755.39 kg. When observed by cow genotypes, the mean values of the lifetime milk production varied from 27,061.37 kg ( (lt) 58% HF) and 24,761.26 kg (58-73% HF) to 23,185.36 kg (>73% HF). The differences in lifetime milk production determined among the animals were due to a highly significant (p≤0.01) impact of the bulls - the sires of the cows and the year of culling; the impact of the class of HF genes was significant (p≤0.05), whereas the impact of the reason for culling was non-significant (p>0.05).Osobine životne proizvodnje visokomlečnih krava imaju veliki ekonomski značaj. Crno-bela goveda imaju značajan genetski potencijal za proizvodnju mleka. Visokoproizvodne krave crno-bele rase izložene su tokom produktivnog veka velikom broju složenih uticaja koji intenzivno deluju na nivo relizacije njihovog genetskog potencijala. Uslovi sredine često nisu u saglasnosti sa potrebama ove visokomlečne rase goveda. Povećanjem učešća gena holštajn-frizijske rase, tokom procesa oplemenjivanja evropskog tipa crno-belih goveda, došlo je do povećanja prinosa mleka. Dosadašnja istraživanja ukazuju na mogućnost za značajnije povećanje životne produktivnosti crno-belih krava. Poznavanje broja i nivoa uticaja faktora sredine, na životnu proizvodnju visokomlečnih krava, važno je zbog njihovog uključivanja u model. U skladu sa značajem pojedinih sistematskih faktora u okviru istraživanja je obavljena njihova objektivna procena. Cilj rada je bio da se primenom odgovarajuće metodologije ispita značajnost razlika, u ostvarenoj životnoj proizvodnji mleka kod visokoproizvodnih crno-belih krava, preko najvažnijih sistematskih uticaja. Prosečna životna proizvodnja mleka iznosila je 25002.66±7755.39 kg litara. Posmatrano po genotipovima krava prosečne vrednosti životne proizvodnje mleka iznosile su 27061.37 kg ( (lt) 58% HF), 24761.26 kg (58-73% HF) i 23185.36 kg (> 73% HF). Utvrđene razlike između grla u pogledu ostvarenog nivoa životne proizvodnje mleka nastale su kao posledica visoko značajnog uticaja (P≤0.01) bikova-očeva krava i godine izlučenja krava, klasa HF gena krava imala je značajan uticaj (p≤0.05), dok razlog izlučenja nije imao značajan uticaj (P>0.05)

Quantum fluctuations of the Chern-Simons theory and dynamical dimensional reduction

We consider a large-N Chern-Simons theory for the attractive bosonic matter (Jackiw-Pi model) in the Hamiltonian collective-field approach based on the 1/N expansion. We show that the dynamics of low-lying density excitations around the ground-state vortex configuration is equivalent to that of the Sutherland model. The relationship between the Chern-Simons coupling constant lambda and the Calogero-Sutherland statistical parameter lambda_s signalizes some sort of statistical transmutation accompanying the dimensional reduction of the initial problem.Comment: 10 pages, 2 figure

Uticaj dodatka Saccharomyces cerevisiae u ishrani na zdravlje i proizvodnju krava tokom perioda tranzicije i početka laktacije

Data concerning the effect of probiotics supplementation on many parameters concurrently at the same cows are lacking. Therefore, the objective of this experiment was to investigate the effects of Saccharomyces cerevisiae feeding on rumen, blood and milk parameters together in high- producing dairy cattle during the transition and early lactation period. Sixteen clinically healthy Holstein cows were divided into 2 groups: a control group of 6 cows and a probiotics-fed group of 10 cows. Rumen fluid and blood samples were collected 21 days before the expected calving as well as 7, 15, 30, 45 and 60 days- in-milk (DIM). Milk yield for each animal was recorded every 2 weeks. Individual milk samples were collected 15, 30, 45 and 60 DIM. Ruminal pH and rumen ammonia nitrogen were significantly lower, whereas total volatile fatty acids were significantly higher in yeast-fed animals compared with controls throughout the study. Serum concentrations of total proteins and globulins were higher, while albumins were lower in the yeast-treated group. Serum glucose levels were significantly higher in yeast-supplemented animals. Serum triglycerides, high density lipoproteins, and low density lipoproteins concentrations were lower, with cholesterol being significantly lower in the treated group. Milk production and milk fat percentage were higher, whereas milk protein percentage and somatic cell count were decreased in yeast-supplemented cows throughout the study. These results suggest that supplementation of S. cerevisiae to dairy cows rations during transition and early lactation period improve their health and milk production parameters.Podaci koji bi se odnosili na uticaj suplementacije probiotika na više parametara istovremeno na istim grlima nedostaju u literaturi. Stoga je cilj ovog eksperimenta bio da se ispita uticaj Saccharomices cerevisiae u obroku/hrani na parametre rumena, krvi i mleka zajedno u visoko-proizvodnim mlečnim govedima tokom tranzicije i početkom laktacije. Šesnaest klinički zdravih holštajn krava je podeljeno u 2 grupe: kontrolna grupa od 6 krava i grupa od 10 krava hranjenih probiotikom u obroku. Buražna tečnost i uzorci krvi su sakupljeni 21 dan pre očekivanog teljenja, kao i 7, 15, 30, 45 i 60 dana tokom laktacije (days in milk - DIM). Prinos mleka za svaku životinju zabeležen je svake 2 nedelje. Pojedinačni uzorci mleka su prikupljeni 15, 30, 45 i 60 DIM. pH buraga i buražni amonijačni azot su bili značajno niži, dok su ukupne isparljive masne kiseline bile značajno veće kod životinja hranjenih kvascem u poređenju sa kontrolama kroz celu studiju. Serumske koncentracije ukupnih proteina i globulina bile su više, dok su koncentracije albumina bile niže u grupi sa kvascem. Serumski nivoi glukoze bili su značajno viši kod životinja sa dodatkom kvasca. Trigliceridi u serumu, koncentracije lipoproteina visoke i niske gustine bile su niže, sa holesterolom koji je bio znatno niži u tretiranoj grupi. Proizvodnja mleka i procent mlečne masti bili su viši, dok je sadržaj proteina mleka i somatskih ćelija bio niži u krava hranjenih sa dodatkom kvasca kroz celu studiju. Ovi rezultati ukazuju na to da dodatak S. cerevisiae u obrocima muznih krava tokom tranzicije i rane laktacije poboljšava njihovo zdravlje i parametre proizvodnje mleka

Uticaj dodatka Saccharomyces cerevisiae u ishrani na zdravlje i proizvodnju krava tokom perioda tranzicije i početka laktacije

Data concerning the effect of probiotics supplementation on many parameters concurrently at the same cows are lacking. Therefore, the objective of this experiment was to investigate the effects of Saccharomyces cerevisiae feeding on rumen, blood and milk parameters together in high- producing dairy cattle during the transition and early lactation period. Sixteen clinically healthy Holstein cows were divided into 2 groups: a control group of 6 cows and a probiotics-fed group of 10 cows. Rumen fluid and blood samples were collected 21 days before the expected calving as well as 7, 15, 30, 45 and 60 days- in-milk (DIM). Milk yield for each animal was recorded every 2 weeks. Individual milk samples were collected 15, 30, 45 and 60 DIM. Ruminal pH and rumen ammonia nitrogen were significantly lower, whereas total volatile fatty acids were significantly higher in yeast-fed animals compared with controls throughout the study. Serum concentrations of total proteins and globulins were higher, while albumins were lower in the yeast-treated group. Serum glucose levels were significantly higher in yeast-supplemented animals. Serum triglycerides, high density lipoproteins, and low density lipoproteins concentrations were lower, with cholesterol being significantly lower in the treated group. Milk production and milk fat percentage were higher, whereas milk protein percentage and somatic cell count were decreased in yeast-supplemented cows throughout the study. These results suggest that supplementation of S. cerevisiae to dairy cows rations during transition and early lactation period improve their health and milk production parameters.Podaci koji bi se odnosili na uticaj suplementacije probiotika na više parametara istovremeno na istim grlima nedostaju u literaturi. Stoga je cilj ovog eksperimenta bio da se ispita uticaj Saccharomices cerevisiae u obroku/hrani na parametre rumena, krvi i mleka zajedno u visoko-proizvodnim mlečnim govedima tokom tranzicije i početkom laktacije. Šesnaest klinički zdravih holštajn krava je podeljeno u 2 grupe: kontrolna grupa od 6 krava i grupa od 10 krava hranjenih probiotikom u obroku. Buražna tečnost i uzorci krvi su sakupljeni 21 dan pre očekivanog teljenja, kao i 7, 15, 30, 45 i 60 dana tokom laktacije (days in milk - DIM). Prinos mleka za svaku životinju zabeležen je svake 2 nedelje. Pojedinačni uzorci mleka su prikupljeni 15, 30, 45 i 60 DIM. pH buraga i buražni amonijačni azot su bili značajno niži, dok su ukupne isparljive masne kiseline bile značajno veće kod životinja hranjenih kvascem u poređenju sa kontrolama kroz celu studiju. Serumske koncentracije ukupnih proteina i globulina bile su više, dok su koncentracije albumina bile niže u grupi sa kvascem. Serumski nivoi glukoze bili su značajno viši kod životinja sa dodatkom kvasca. Trigliceridi u serumu, koncentracije lipoproteina visoke i niske gustine bile su niže, sa holesterolom koji je bio znatno niži u tretiranoj grupi. Proizvodnja mleka i procent mlečne masti bili su viši, dok je sadržaj proteina mleka i somatskih ćelija bio niži u krava hranjenih sa dodatkom kvasca kroz celu studiju. Ovi rezultati ukazuju na to da dodatak S. cerevisiae u obrocima muznih krava tokom tranzicije i rane laktacije poboljšava njihovo zdravlje i parametre proizvodnje mleka

Duality and quasiparticles in the Calogero-Sutherland model: Some exact results

The quantum-mechanical many-body system with the potential proportional to the pairwise inverse-square distance possesses a strong-weak coupling duality. Based on this duality, particle and/or quasiparticle states are described as SU(1,1) coherent states. The constructed quasiparticle states are of hierarchical nature.Comment: RevTeX, 10 page

Razvoj sposobnosti atomsko-biološko-hemijske službe u odgovoru na pretnje hemijskim oružjem

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Kembrička kristalografska banka podataka (CSD)

Department of Chemistry, University of Belgrade, Serbia Cambridge Structural Database (CSD) contains more than five hundred thousand crystal structures of small molecules. Around 150 new crystal structures are archived every day. The data archived in the CSD can help in various fields of research. Some of them are crystallography, structural chemistry, supramolecular chemistry, noncovalent interactions, drug design, and metal-ligand interactions. Using CSD data and informatics approach enables studies based on analysis of a large amount of data