Calcium and the control of C4 photosynthesis

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Synthesis of (9Z, 12E)-, (9E, 12Z)-[1-14C]-linoleic acid, (9Z, 12Z, 15E)-, (9E, 12Z, 15Z)-[1-14C]-linolenic acid and (5Z, 8Z, 11Z, 14E)-[1-14C]-arachidonic acid

Trans polyunsaturated fatty acids are produced in vegetable oils during heat treatment (240-250 °C).ln order to study the metabolic pathway of 9c, 12t and 9t, 12c linoleic acid and 9c, 12c, 15t and 9t, 12c, 15c linolenic acid, these products were prepared labelled with carbon 14 in the carboxylic position. 5c, 8c, 11c, 14t-Arachidonic acid was also labelled on the carboxylic position with carbon 14 in order to study its physiological effects. To introduce the labelling (E)-bromo precursors with a 17 carbons chain or a 19 carbon chain were needed. The different syntheses were done by elongation steps and creation of cis double bonds via highly stereospecific Wittig reactions. The radioactive carbon atom was introduced from [14C]-potassium cyanide. The final radioactive fatty acids had a specific activity greater than 50 mCi/mmol and a radioactive purity better than 99 % for linoleic and linolenic and better than 98.6 % for arachidonic acid

La seizième campagne de fouilles de la Casa de Velázquez à Belo en 1981 (Bolonia, province de Cadix)

Bonneville Jean-Nöel, Didierjean François, Le Roux Patrick, Rouillard Pierre, Sillières Pierre. La seizième campagne de fouilles de la Casa de Velázquez à Belo en 1981 (Bolonia, province de Cadix). In: Mélanges de la Casa de Velázquez, tome 18-2, 1982. pp. 5-65

Chronique: La vingtième campagne de fouilles de la casa de Velázquez à Belo en 1985 ( Bolonia, province de Cadix )

Pelletier Agnès, Dardaine Sylvie, Bonneville Jean-Nöel, Jacob Pierre, Fincker Myriam, Sillières Pierre, Paillet Jean-Louis. Chronique: La vingtième campagne de fouilles de la casa de Velázquez à Belo en 1985 ( Bolonia, province de Cadix ). In: Mélanges de la Casa de Velázquez, tome 22, 1986. pp. 495-520

La quinzième campagne de fouilles de la Casa de Velázquez à Belo en 1980 (Bolonia, province de Cadix)

Bonneville Jean-Nöel, Dardaine Sylvie, Didierjean François, Le Roux Patrick, Sillières Pierre. La quinzième campagne de fouilles de la Casa de Velázquez à Belo en 1980 (Bolonia, province de Cadix). In: Mélanges de la Casa de Velázquez, tome 17, 1981. pp. 393-456

La dix-neuvième campagne de fouilles de la Casa de Velázquez à Belo en 1984 ( Bolonia, province de Cadix )

Le Roux Patrick, Lancha Janine, Bonneville Jean-Nöel, Jacob Pierre, Fincker Myriam, Paillet Jean-Louis. La dix-neuvième campagne de fouilles de la Casa de Velázquez à Belo en 1984 ( Bolonia, province de Cadix ). In: Mélanges de la Casa de Velázquez, tome 21, 1985. pp. 347-369

Effects of trans n-6 fatty acids on the fatty acid profile of tissues and liver microsomal desaturation in the rat

18:2Δ 9c,12t and 18:2 Δ9t,12c are present in our diet, as result of heat treatment of vegetable oils. A nutritional study was carried out in order to obtain more precise information on the conversion of these two isomers into long chain polyunsaturated fatty acids (PUFA) by rat tissues. This in vivo study performed using rat fed with small quantities of mono trans linoleic acid isomers (0.6% of total energy) showed that 18:2 Δ9c,12t was converted into 20:4 Δ5c,8c,11c,14t while 18:2 Δ9t,12c was only slightly converted into 20:4 Δ5c,8c,11t,14c. Furthermore 18:2 Δ9t,12c was preferentially elongated into 20:2 Δ11t,14c. Each C20 metabolite of these mono trans 18:2 isomers was isolated as methyl ester by semi-preparative high-performance liquid chromatography (HPLC) followed by silver nitrate thin layer chromatography (AgNO3-TLC).The structure of the components was identified using partial hydrazine reduction, AgNO3-TLC of the resulting monoenes and gas-liquid chromatography coupled with mass spectrometry (GC-MS) of the 4,4-dimethyloxazoline (DMOX) derivatives. Fourier-transform-infrared spectroscopy (GC-FTIR) confirmed the frans geometry. Gas-liquid chromatography (GC) analyses showed that 18:2 Δ9c,12t and 18:2 Δ9t,12c were present in different tissue lipids (liver, heart, testes, brain and adipose tissue), and without any modification in the amount of 20:4n-6. 20:4 Δ5c, 8c,11c,14t was incorporated in different rat tissues except in brain. Furthermore, its incorporation followed that of its structural analogue, 20:3n-9 in liver phospholipid classes (phosphatidylethanolamine, phosphatidylinositol and phosphatidylcholine). Finally, an in vitro study carried out with rat liver microsomes showed that dietary trans 18:2 isomers could inhibit the Δ6- desaturation of 18:2n-6 to 18:3n-6 and the Δ5-desaturation of 20:3n-6 to 20:4n-6