Cytokine profile of spleen T-lymphocytes from mice before (0) and at 7, 14, 28 and 42 days after infection with metacyclic (MT) or blood trypomastigotes (BT) of <i>Trypanosoma cruzi</i>.

<p>“Gray scale” diagrams were used to represent the cytokine pattern and the cytokine balance within T-cell subsets besides the overall cytokine balance with T-cells, highlighting the predominance of “low” cytokine-producers (white square), “high” TNF-α or IFN-γ producers (black square), “high” IL-10⁺ producers (light gray square) or “high” mixed cytokine-producers (dark gray square). Pie charts represent the percentage of animals displaying a given T-cells overall cytokine balance selectively amongst the “high” cytokine-producers. ND = Not detected.</p

Morphometric analysis and photomicrographs of the area of <i>T. cruzi</i> immunoreactions in the heart at 28 days after mice infection with metacyclic (MT; light gray bar) or blood trypomastigotes (BT; black bar) of <i>Trypanosoma cruzi</i>.

<p>Analysis of the area of <i>T. cruzi</i> amastigotes were identified by immunohistochemistry as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032912#s4" target="_blank">Material and Methods</a>. The results are expressed as parasited area ± standard error. Photomicrographs of the heart parasitism demonstrating isolated amastigotes in MT and typical amastigote nests in BT.</p