1 research outputs found
Influence of Solution Chemistry and Soft Protein Coronas on the Interactions of Silver Nanoparticles with Model Biological Membranes
The influence of solution chemistry
and soft protein coronas on
the interactions between citrate-coated silver nanoparticles (AgNPs)
and model biological membranes was investigated by assembling supported
lipid bilayers (SLBs) composed of zwitterionic 1,2-dioleoyl-<i>sn</i>-glycero-3-phosphocholine (DOPC) on silica crystal sensors
in a quartz crystal microbalance with dissipation monitoring (QCM-D).
Our results show that the deposition rates of AgNPs on unmodified
silica surfaces increased with increasing electrolyte concentrations
under neutral pH conditions. Similar trends were observed when AgNPs
were deposited on SLBs, hence indicating that the deposition of AgNPs
on model cell membranes was controlled by electrostatic interactions.
In the presence of human serum albumin (HSA) proteins at both pH 7
and pH 2, the colloidal stability of AgNPs was considerably enhanced
due to the formation of HSA soft coronas surrounding the nanoparticles.
At pH 7, the deposition of AgNPs on SLBs was suppressed in the presence
of HSA due to steric repulsion between HSA-modified AgNPs and SLBs.
In contrast, pronounced deposition of HSA-modified AgNPs on SLBs was
observed at pH 2. This observation was attributed to the reduction
of electrostatic repulsion as well as conformation changes of adsorbed
HSA under low pH conditions, resulting in the decrease of steric repulsion
between AgNPs and SLBs