A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample-2

<p><b>Copyright information:</b></p><p>Taken from "A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample"</p><p>BMC Cancer 2005;5():122-122.</p><p>Published online 22 Sep 2005</p><p>PMCID:PMC1261259.</p><p>Copyright © 2005 Eidukevicius et al; licensee BioMed Central Ltd.</p>values obtained with the proposed method and with the RM (cubic) method. C. Correlation between values obtained with the proposed method and with the RM (cubic) method

A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample-3

<p><b>Copyright information:</b></p><p>Taken from "A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample"</p><p>BMC Cancer 2005;5():122-122.</p><p>Published online 22 Sep 2005</p><p>PMCID:PMC1261259.</p><p>Copyright © 2005 Eidukevicius et al; licensee BioMed Central Ltd.</p

A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample-0

<p><b>Copyright information:</b></p><p>Taken from "A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample"</p><p>BMC Cancer 2005;5():122-122.</p><p>Published online 22 Sep 2005</p><p>PMCID:PMC1261259.</p><p>Copyright © 2005 Eidukevicius et al; licensee BioMed Central Ltd.</p>t < T. , Tand are durations of G, S and Gphases of the cell cycle, respectively. Horizontal lines dividing Gand Sbars indicate that half of the labelled divided cells are excluded from calculations

A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample-1

<p><b>Copyright information:</b></p><p>Taken from "A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample"</p><p>BMC Cancer 2005;5():122-122.</p><p>Published online 22 Sep 2005</p><p>PMCID:PMC1261259.</p><p>Copyright © 2005 Eidukevicius et al; licensee BioMed Central Ltd.</p>orescence) with a gate set for BrdUrd positive cells. B. DNA histogram analysis of BrdUrd positive cells; M1 indicates labelled divided Gcells (G); M2 indicates labelled divided S cells (S); M3 indicates labelled undivided S cells (S); M4 indicates labelled undivided Gcells (G). C. The same DNA histogram as in B, shown at higher magnification; Scompartment is separated from Scompartment by the channel corresponding to the lowest number of events (indicated by dotted vertical line). Total number of cells within each of these markers is obtained using statistics option of WinMDI 2.8 software

A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample-4

<p><b>Copyright information:</b></p><p>Taken from "A method to estimate cell cycle time and growth fraction using bromodeoxyuridine-flow cytometry data from a single sample"</p><p>BMC Cancer 2005;5():122-122.</p><p>Published online 22 Sep 2005</p><p>PMCID:PMC1261259.</p><p>Copyright © 2005 Eidukevicius et al; licensee BioMed Central Ltd.</p> Labelled cells have not yet entered the Scompartment. B. Labelled cells start entering the Scompartment. C. Considerable proportions of labelled cells are present both in the Sand in the Scompartment. D. Labelled cells are leaving the Scompartment. The optimal time for measurement of cell kinetics in Jurkat cells with the proposed method appears to be 12 h