Blind estimation of reverberation time in classrooms and hospital wards

This paper investigates blind Reverberation Time (RT) estimation in occupied classrooms and hospital wards. Measurements are usually made while these spaces are unoccupied for logistical reasons. However, occupancy can have a significant impact on the rate of reverberant decay. Recent work has developed a Maximum Likelihood Estimation (MLE) method which utilises only passively recorded speech and music signals, this enables measurements to be made while the room is in use. In this paper the MLE method is applied to recordings made in classrooms during lessons. Classroom occupancy levels differ for each lesson, therefore a model is developed using blind estimates to predict the RT for any occupancy level to within ±0.07s for the mid-frequency octave bands. The model is also able to predict the effective room and per person absorption area. Ambient sound recordings were also carried out in a number of rooms in two hospitals for a week. Hospital measurements are more challenging as the occurrence of free reverberant decay is rarer than in schools and the acoustic conditions may be non-stationary. However, by gaining recordings over a period of a week, estimates can be gained within ±0.07 s. These estimates are representative of the times when the room contains the highest acoustic absorption. In other words when curtains are drawn, there are many visitors or perhaps a window may be open

An integer programming approach for the 2-class single-group classification problem

Two sets XB, XR ⊆ Rd are linearly separable if their convex hulls are disjoint, implying that a hyperplane separating XB from XR exists. Such a hyperplane provides a method for classifying new points, according to the side of the hyperplane in which the new points lie. In this work we consider a particular case of the 2-class classification problem, which asks to select the maximum number of points from XB and XR in such a way that the selected points are linearly separable. We present an integer programming formulation for this problem, explore valid inequalities for the associated polytope, and develop a cutting plane approach coupled with a lazy-constraints scheme.Fil: Corrêa, Ricardo C.. Universidade Federal Rural Do Rio de Janeiro; BrasilFil: Blaum, Manuela. Universidad Nacional de General Sarmiento; ArgentinaFil: Marenco, Javier Leonardo. Universidad Nacional de General Sarmiento; ArgentinaFil: Koch, Ivo Valerio. Universidad Nacional de General Sarmiento; ArgentinaFil: Mydlarz, Marcelo. Universidad Nacional de General Sarmiento; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaLatin-American Algorithms, Graphs and Optimization Symposium (LAGOS 2019)Belo HorizonteBrasilCoordenação de Aperfeiçoamento de Pessoal de Nivel SuperiorConselho Nacional de Desenvolvimento Científico e Técnologico do BrasilUniversidade Federal de Minas Gerai

Cellular Responses in Sea Fan Corals: Granular Amoebocytes React to Pathogen and Climate Stressors

BACKGROUND: Climate warming is causing environmental change making both marine and terrestrial organisms, and even humans, more susceptible to emerging diseases. Coral reefs are among the most impacted ecosystems by climate stress, and immunity of corals, the most ancient of metazoans, is poorly known. Although coral mortality due to infectious diseases and temperature-related stress is on the rise, the immune effector mechanisms that contribute to the resistance of corals to such events remain elusive. In the Caribbean sea fan corals (Anthozoa, Alcyonacea: Gorgoniidae), the cell-based immune defenses are granular acidophilic amoebocytes, which are known to be involved in wound repair and histocompatibility. METHODOLOGY/PRINCIPAL FINDINGS: We demonstrate for the first time in corals that these cells are involved in the organismal response to pathogenic and temperature stress. In sea fans with both naturally occurring infections and experimental inoculations with the fungal pathogen Aspergillus sydowii, an inflammatory response, characterized by a massive increase of amoebocytes, was evident near infections. Melanosomes were detected in amoebocytes adjacent to protective melanin bands in infected sea fans; neither was present in uninfected fans. In naturally infected sea fans a concurrent increase in prophenoloxidase activity was detected in infected tissues with dense amoebocytes. Sea fans sampled in the field during the 2005 Caribbean Bleaching Event (a once-in-hundred-year climate event) responded to heat stress with a systemic increase in amoebocytes and amoebocyte densities were also increased by elevated temperature stress in lab experiments. CONCLUSIONS/SIGNIFICANCE: The observed amoebocyte responses indicate that sea fan corals use cellular defenses to combat fungal infection and temperature stress. The ability to mount an inflammatory response may be a contributing factor that allowed the survival of even infected sea fan corals during a stressful climate event

The impact of classroom noise on reading comprehension of secondary school pupils

It has been known for many years that poor acoustic conditions in classrooms leading to high noise levels and poor speech intelligibility cause annoyance to pupils and teachers and affect the academic performance of pupils. Much of the previous research concerning the impact of noise and poor acoustics on pupils has involved children in primary schools, with fewer studies related to pupils of secondary school age. Furthermore, the majority of previous studies in schools have examined the impact of environmental noise, particularly aircraft noise, on children. The aim of the study described here was to examine the effects of typical levels of classroom noise on secondary school pupils, and to attempt to identify the threshold level at which adverse impacts might occur. A survey of acoustic conditions in secondary schools in England1 was accompanied by questionnaire surveys of pupils to ascertain levels of annoyance caused by noise, and their perceptions of its interference with their ability to hear and understand their teachers2 . In addition, students were tested in numeracy, mathematical reasoning, memory and reading comprehension in different levels of classroom noise. This paper describes the results of reading comprehension tests undertaken by nearly 1000 pupils aged between 11 and 16 years while they were exposed to typical classroom noise at different levels

Deciphering coral disease dynamics: integrating host, microbiome, and the changing environment

Diseases of tropical reef organisms is an intensive area of study, but despite significant advances in methodology and the global knowledge base, identifying the proximate causes of disease outbreaks remains difficult. The dynamics of infectious wildlife diseases are known to be influenced by shifting interactions among the host, pathogen, and other members of the microbiome, and a collective body of work clearly demonstrates that this is also the case for the main foundation species on reefs, corals. Yet, among wildlife, outbreaks of coral diseases stand out as being driven largely by a changing environment. These outbreaks contributed not only to significant losses of coral species but also to whole ecosystem regime shifts. Here we suggest that to better decipher the disease dynamics of corals, we must integrate more holistic and modern paradigms that consider multiple and variable interactions among the three major players in epizootics: the host, its associated microbiome, and the environment. In this perspective, we discuss how expanding the pathogen component of the classic host-pathogen-environment disease triad to incorporate shifts in the microbiome leading to dysbiosis provides a better model for understanding coral disease dynamics. We outline and discuss issues arising when evaluating each component of this trio and make suggestions for bridging gaps between them. We further suggest that to best tackle these challenges, researchers must adjust standard paradigms, like the classic one pathogen-one disease model, that, to date, have been ineffectual at uncovering many of the emergent properties of coral reef disease dynamics. Lastly, we make recommendations for ways forward in the fields of marine disease ecology and the future of coral reef conservation and restoration given these observations

Complementary approaches to diagnosing marine diseases: a union of the modern and the classic

Linking marine epizootics to a specific aetiology is notoriously difficult. Recent diagnostic successes show that marine disease diagnosis requires both modern, cutting-edge technology (e.g. metagenomics, quantitative realtime PCR) and more classic methods (e.g. transect surveys, histopathology and cell culture). Here, we discuss how this combination of traditional and modern approaches is necessary for rapid and accurate identification of marine diseases, and emphasize how sole reliance on any one technology or technique may lead disease investigations astray. We present diagnostic approaches at different scales, from the macro (environment, community, population and organismal scales) to the micro (tissue, organ, cell and genomic scales). We use disease case studies from a broad range of taxa to illustrate diagnostic successes from combining traditional and modern diagnostic methods. Finally, we recognize the need for increased capacity of centralized databases, networks, data repositories and contingency plans for diagnosis and management of marine disease.Keywords: marine epizootics, aetiology, marine disease, diagnosticsKeywords: marine epizootics, aetiology, marine disease, diagnostic

Serum microrna biomarkers for detection of non-small cell lung cancer

Non small cell lung cancer (NSCLC) is the leading cause of cancer-related mortality world-wide and the majority of cases are diagnosed at late stages of disease. There is currently no cost-effective screening test for NSCLC, and the development of such a test is a public health imperative. Recent studies have suggested that chest computed tomography screening of patients at high risk of lung cancer can increase survival from disease, however, the cost effectiveness of such screening has not been established. In this Phase I/II biomarker study we examined the feasibility of using serum miRNA as biomarkers of NSCLC using RT-qPCR to examine the expression of 180 miRNAs in sera from 30 treatment naive NSCLC patients and 20 healthy controls. Receiver operating characteristic curves (ROC) and area under the curve were used to identify differentially expressed miRNA pairs that could distinguish NSCLC from healthy controls. Selected miRNA candidates were further validated in sera from an additional 55 NSCLC patients and 75 healthy controls. Examination of miRNA expression levels in serum from a multi-institutional cohort of 50 subjects (30 NSCLC patients and 20 healthy controls) identified differentially expressed miRNAs. A combination of two differentially expressed miRNAs miR-15b and miR-27b, was able to discriminate NSCLC from healthy controls with sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 100% in the training set. Upon further testing on additional 130 subjects (55 NSCLC and 75 healthy controls), this miRNA pair predicted NSCLC with a specificity of 84% (95% CI 0.73-0.91), sensitivity of 100% (95% CI; 0.93-1.0), NPV of 100%, and PPV of 82%. These data provide evidence that serum miRNAs have the potential to be sensitive, cost-effective biomarkers for the early detection of NSCLC. Further testing in a Phase III biomarker study in is necessary for validation of these results. © 2012 Hennessey et al

Quantitative Methylation Profiles for Multiple Tumor Suppressor Gene Promoters in Salivary Gland Tumors

Methylation profiling of tumor suppressor gene (TSGs) promoters is quickly becoming a powerful diagnostic tool for the early detection, prognosis, and even prediction of clinical response to treatment. Few studies address this in salivary gland tumors (SGTs); hence the promoter methylation profile of various TSGs was quantitatively assessed in primary SGT tissue to determine if tumor-specific alterations could be detected.DNA isolated from 78 tumor and 17 normal parotid gland specimens was assayed for promoter methylation status of 19 TSGs by fluorescence-based, quantitative methylation-specific PCR (qMSP). The data were utilized in a binary fashion as well as quantitatively (using a methylation quotient) allowing for better profiling and interpretation of results..Screening promoter methylation profiles in SGTs showed considerable heterogeneity. The methylation status of certain markers was surprisingly high in even normal salivary tissue, confirming the need for such controls. Several TSGs were found to be associated with malignant SGTs, especially SDC. Further study is needed to evaluate the potential use of these associations in the detection, prognosis, and therapeutic outcome of these rare tumors