Twin-screw Extrusion Processing of Vegetable-Based Protein Feeds for Yellow Perch ( Perca flavescens) Containing Distillers Dried Grains (DDG), Soy Protein Concentrate (SPC), and Fermented High Protein Soybean Meal (FSBM)

Changing to alternative protein sources supports production of more economic aquafeeds. Two isocaloric (3.06 kcal/g) and isonitrogenous (40% db) experimental feeds for juvenile yellow perch were formulated with incorporation of FSBM and SPC, each of which were at two levels (0 and 20% db), along with constant amounts of high protein DDG (~30% db), and appropriate amounts of other ingredients. Using a pilot scale twin-screw extruder, feed production was performed in two replications for each diet at conditioner steam levels of 0.11 to 0.16 kg/min, extruder water of 0.11 to 0.19 kg/min, and screw speeds of 230 to 300 rpm. The effects of SPC and FSBM inclusions on extrudate physical properties were compared with those of a control diet (which contained 20% fishmeal and ~30% DDG). Inclusion of 20% FSBM and 20%SPC resulted in a substantial decrease in unit density by 9.2 and 24%, but an increase in lightness, greenness, yellowness, and expansion ratio of the extrudates by 7, 27, 14, 7, 17, 34, 15, and 16.5%, respectively. SPC inclusion led to a considerable increase in water absorption, thermal resistivity, and thermal diffusivity by 17.5, 6.3, and 17.6%, respectively, whereas no significant change was observed for these properties with incorporation of 20% FSBM. Additionally, all extruded products had high durability. Taken together, using ~30% DDG with 20% FSBM or 20% SPC as alternative protein sources resulted in viable extrudates with properties appropriate for yellow perch production. A future study investigating the effect of extrusion processing conditions on the production of complete vegetable-based protein feeds for yellow perch species would be appropriate

Twin-screw Extrusion Processing of Vegetable-Based Rainbow trout (Oncorhynchus mykiss) Feeds Using Graded Levels of High Protein Fermented Soybean Meal (FSBM)

Fast-paced growth in global aquaculture has elevated concerns about the high costs of aquafarm production and potential water pollution. Thus, finding eco-friendly and more sustainable alternative protein sources for fish diets is of vital importance to the industry. A twin-screw extrusion processing study was performed using three ingredient blends formulated with graded levels of FSBM (0, 80% and 100% db) as the fishmeal replacer, in combination with appropriate amounts of other required ingredients for rainbow trout diets. Increasing the FSBM content from 0% to 100% resulted in a substantial increase in brightness, greenness, and yellowness, and a decrease in bulk density, water absorption index, and unit density (UD) values of the extrudates by 12.5%, 73%, 30%, 7.3%, 27.5%, and 10%, respectively. Compared to the control diet (100% fishmeal-based), extrudate moisture contents increased by 15.2% and 22% for the diets containing 80 and 100% FSBM, respectively; although no change was observed by increasing FSBM from 80 to 100%. The highest water solubility index (WSI) was obtained for 80% FSBM; however, further increasing FSBM did not influence the WSI significantly. All extrudates exhibited low water activity and high pellet durability values (less than 0.5 and more than 99.5%, respectively). The most buoyant extrudates were obtained using total FSBM inclusion, with UD and expansion ratio values of nearly 660 kg/m3 and 1.3, respectively. Overall, results indicated that FSBM can be a promising alternative protein in rainbow trout feed production

Modeling Single-Screw Extrusion Processing Parameters and Resulting Extrudate Properties of DDGS-Based Nile Tilapia (Oreochromis niloticus) Feeds

A single-screw laboratory extruder was used to conduct an L18 (22´ 36) Taguchi fractional factorial study of aquafeed processing. The ingredients were based on a formulation for nutritionally-balanced Nile tilapia diets containing distillers dried grains with solubles (DDGS) and soybean meal as the main protein sources, in addition to constant amounts of corn flour, whey, and fishmeal. The effects of three levels of DDGS (20, 30 and 40%), soybean meal (30, 40 and 50%), ingredient moisture content (20, 30 and 40% db), screw speed (100, 150 and 200 rpm), die dimension (L/D ratios of 5, 9 and 13), barrel temperature (80-100-100°C, 80-120-120°C and 80-140-140°C) and two levels of screw configuration (compression ratios of 2:1 and 3:1) on extrudate physical properties (moisture content, water activity, bulk density, unit density, expansion ratio, pellet durability index, water absorption and solubility indices, water stability, color) and extruder processing parameters (resulting temperatures, die pressure, extruder torque, mass flow rate, apparent viscosity, and specific mechanical energy) were determined. Data from raw materials, processing conditions, and extrudate properties were used to develop surface response curves and equations. However, predominantly low R2values (\u3c 0.5) only permitted linear relationships between some independent parameters and response variables. Regarding main effects, die pressure significantly decreased with higher DDGS levels, moisture content, temperature, lower die L/D, and higher screw compression. Expansion ratio decreased significantly with higher moisture content and lower die L/D. Significant differences in color were caused by changes in DDGS levels and moisture content. In summary, DDGS, moisture content, die dimension, and extrusion conditions had the biggest impact on most of the extrudate physical properties and processing conditions. Different combinations of these independent factors can be used to achieve desired extrudate physical properties and processing conditions

Twin-screw Extrusion Processing of Rainbow trout (Oncorhynchus mykiss) feeds using Graded Levels of High Protein Corn-based Distillers Dried Grains (HP-DDG) and Conventional Distillers Dried Grains with Solubles (DDGS)

Twin-screw extrusion cooking trials were performed to investigate the differential effects of conventional DDGS (DDGS) and HP-DDG inclusion, as fishmeal replacers, on physical properties (moisture content, water activity, thermal properties, expansion ratio, unit density, bulk density, water absorption, solubility and pellet durability indices, and color) of Rainbow trout (Oncorhynchus mykiss) feed. Four ingredient blends were formulated with 20 and 40% C- DDGS, and 20 and 40% HP-DDG, along with other required dietary ingredients. Each diet was extruded using two replications; a fishmeal based diet acted as the control diet. The highest and the lowest extrudate moisture content and water activity were observed for the diets containing 20% DDGS and 40% HP-DDG, respectively. Compared to the control diet, increasing HP-DDG content from 20 to 40% had no effect on the bulk density of the products, while increasing DDGS content from 20-40% led to a considerable rise in bulk density of the extrudates by 14.2 and 6%, respectively. Also, extrudates with the lowest brightness, greenness, and yellowness values were obtained from the diet containing 20% DDGS, whereas the most color intensity values were observed for the diets containing 40% DDGS and 20% HP-DDG. Increasing DDGS and HP-DDG from 0-40% caused a remarkable increase in water absorption index, by 72 and 30%, respectively. Likewise, the water solubility index increased, but in a considerably lesser order of magnitude. High pellet durability index of more than 99% was achieved with inclusion of either DDGS or HP-DDG. None of the diets impacted the thermal properties of the extrudates. Inclusion of 20 and 40% DDGS resulted in the extrudates with the largest and the smallest unit density values of nearly 887 and 750 kg/m3, respectively. As was expected, expansion ratio decreased with increasing unit density. Increasing inclusion levels of HP-DDG did not show any effect on unit density or expansion ratio of the products. Future research should concentrate on investigating the effects of both extrusion processing and graded levels of different types of DDGS on Rainbow trout feeds

Pyrolysis of Distillers Grains

As the quantity of ethanol produced in the U.S. continues to expand, so too does the need for value-added applications for the resulting coproducts. Distillers dried grains with solubles (DDGS), corn gluten meal (CGM), and corn gluten feed (CGF) are the primary coproducts of ethanol manufacturing (which includes dry grind processing and wet milling). The main consumer of these coproducts is the livestock industry, which utilizes these materials as animal feed and ingredients due to their high nutrient contents, good palatability and digestibility, and relatively low cost; however, new value added uses are starting to arise. This study will examine the pyrolysis of DDGS and CGM. Extensive characterization of the resulting bio-oil included mass density, thermal conductivity, thermal diffusivity, apparent viscosity, and energy content. Potential opportunities for extracting value from these materials will also be explored and discussed

Effects of varying condensed distillers solubles, drying, and cooling temperatures on glass transition temperature of distillers dried grains

Distillers dried grains with solubles (DDGS), a coproduct of ethanol fuel production, is used as an animal feed and often must be transported long distances. DDGS flowability problems often create nuisance in storage and transportation. Materials above the glass transition temperature (Tg) can exist in a “rubbery state,”’ which is often responsible for particle agglomeration and caking. This study investigated the effects of varying condensed distillers solubles (CDS) (10, 15, and 20%, wb), drying (100, 200, and 300°C), and cooling temperature (–12 and 35°C) levels on the Tg of DDGS. Tg ranged from 34 to 58°C and 41 to 59°C for cooling temperatures of –12°C and 35°C, respectively. Tg data were used to develop an overall regression model, which yielded a predictive model with R² of 0.74 and SEM of 3.16. Using this model, optimum drying and cooling temperatures were determined. These conditions may be used to reduce flow problems

Techno-Economic Modeling of Coproduct Processing in a Corn Based Ethanol Plant in 2012

Coproducts, such as Distillers Dried Grains with Solubles (DDGS), produced during ethanol production are essential to the economic sustainability of each ethanol plant as they provide an additional source of revenue. DDGS is extensively used as animal feed, but has a relatively low market value compared to the biofuel. By fractioning DDGS into lighter and heavier fractions, the overall composition changes potentially increasing the value of the coproducts as they become more desirable to different markets. Earlier studies have examined fractionating DDGS using sieves and aspirators. This project examined the techno-economics of adding fractionation systems onto an existing 40 million gal/y ethanol plant. The model allowed for estimations of fixed capital costs, annual operating costs, annual revenues, and net profits, in order to determine the economic feasibility of adding three different fractionation systems. The first fractionation system consisted of a single sieve, and the retained material was passed through an aspirator. The second system was similar to the first but with a second sieve and aspirator. The third system added a third set of them. In addition to utilizing different fractionation systems, the scenarios examined the effects extracting corn oil and producing DWG in addition to DDGS. The fractionation systems examined in this study increased the capital costs associated with the facility, but did not greatly affect the overall annual operating costs. The net profits in the four most profitable scenarios were $0.349/gal EtOH/y (scenario 14),$0.350/gal EtOH/y (scenarios 6 and 10), and $0.351/gal EtOH/y (scenario 2)

Twin-screw Extrusion Processing of Vegetable-based Protein Feeds for Yellow Perch (Perca flavescens) Containing Distillers Dried Grains, Soy Protein Concentrate, and Fermented High Protein Soybean Meal

Changing to alternative protein sources supports production of more economic aquafeeds. Two isocaloric (3.06 kcal/g) and isonitrogenous (40% db) experimental feeds for juvenile yellow perch were formulated with incorporation of fermented soybean meal (FSBM) and soy protein concentrate (SPC), each of which were at two levels (0 and 20% db), along with constant amounts of high protein distillers dried grains (DDG) (~30% db), and appropriate amounts of other ingredients. Using a pilot scale twin-screw extruder, feed production was performed in two replications for each diet at conditioner steam levels of 0.11 to 0.16 kg/min, extruder water of 0.11 to 0.19 kg/min, and screw speeds of 230 to 300 rpm. The effects of SPC and FSBM inclusion on extrudate physical properties were compared with those of a control diet (which contained 20% fishmeal and ~30% DDG). Inclusion of 20% FSBM and 20%SPC resulted in a substantial decrease in unit density by 9.2 and 24%, but an increase in lightness, greenness, yellowness, and expansion ratio of the extrudates by 7, 27, 14, 7, 17, 34, 15, and 16.5%, respectively. SPC inclusion led to a considerable increase in water absorption, thermal resistivity, and thermal diffusivity by 17.5, 6.3, and 17.6%, respectively, whereas no significant change was observed for these properties with incorporation of 20% FSBM. Additionally, all extruded products had high durability. Taken together, using ~30% DDG with20% FSBM or20% SPC as alternative protein sources resulted in viable extrudates with properties appropriate for yellow perch production. A future study investigating the effect of extrusion processing conditions on the production of complete vegetable-based protein feeds for yellow perch species would be appropriate

Techno-Economic Modeling of a Corn Based Ethanol Plant in 2011

As studies continue to examine new value added uses for ethanol coproducts, it is important to have means to easily determine the feasibility of the processing steps involved. Many industries widely use computer simulation programs for this purpose, and for planning the use of resources and equipment capacities, and to determine processing costs. The objective of this project was to determine the sensitivity of 40 million gal/y corn-based ethanol plant model to changes in input material prices, product market prices, and various coproduct processing scenarios (i.e., oil extraction and drying of DDGS). The techno-economics of the base case ethanol plant were examined by factorially adjusting material and market costs, as well as adjusting the quantities of distillers wet grains (DWG), distillers dried grains with solubles (DDGS), and corn oil produced. The simulations verified that corn price has the greatest impact on the overall annual operating costs for the ethanol plant, and that the market price of ethanol has the greatest impact on annual revenues. The effect of coproduct processing on utility usage was also observed; oil extraction and drying of DDGS consumed substantially more energy and had higher capital costs than production of DWG alone. It was apparent that coproducts are an essential component to the sustainability of an ethanol plant in that: 1) they have continued marketability to the livestock industry, and 2) processing is not overly-expensive. This study has provided a basis for further exploration of the feasibility of new coproduct processing options, and illustrates the use of the model for determination of processing costs and revenues, as well as mass and energy balances

Twin-Screw Extrusion Processing of Rainbow trout (Oncorhynchus mykiss) Feeds Using Graded Levels of High Protein Corn-Based Distillers Dried Grains (HP-DDG) and Conventional Distillers Dried Grains with Solubles (DDGS)

Twin-screw extrusion cooking trials were performed to investigate the differential effects of conventional DDGS (DDGS) and HP-DDG inclusion, as fishmeal replacers, on physical properties (moisture content, water activity, thermal properties, expansion ratio, unit density, bulk density, water absorption, solubility and pellet durability indices, and color) of Rainbow trout (Oncorhynchus mykiss) feed. Four ingredient blends were formulated with 20 and 40% C- DDGS, and 20 and 40% HP-DDG, along with other required dietary ingredients. Each diet was extruded using two replications; a fishmeal based diet acted as the control diet. The highest and the lowest extrudate moisture content and water activity were observed for the diets containing 20% DDGS and 40% HP-DDG, respectively. Compared to the control diet, increasing HP-DDG content from 20 to 40% had no effect on the bulk density of the products, while increasing DDGS content from 20-40% led to a considerable rise in bulk density of the extrudates by 14.2 and 6%, respectively. Also, extrudates with the lowest brightness, greenness, and yellowness values were obtained from the diet containing 20% DDGS, whereas the most color intensity values were observed for the diets containing 40% DDGS and 20% HP-DDG. Increasing DDGS and HP-DDG from 0-40% caused a remarkable increase in water absorption index, by 72 and 30%, respectively. Likewise, the water solubility index increased, but in a considerably lesser order of magnitude. High pellet durability index of more than 99% was achieved with inclusion of either DDGS or HP-DDG. None of the diets impacted the thermal properties of the extrudates. Inclusion of 20 and 40% DDGS resulted in the extrudates with the largest and the smallest unit density values of nearly 887 and 750 kg/m3, respectively. As was expected, expansion ratio decreased with increasing unit density. Increasing inclusion levels of HP-DDG did not show any effect on unit density or expansion ratio of the products. Future research should concentrate on investigating the effects of both extrusion processing and graded levels of different types of DDGS on Rainbow trout feeds