2 research outputs found
TECHNICAL NOTE - Single embryoid body formation in a multi-well plate
Embryoid bodies (EB) formed from murine embryonic stem (ES) cells
recapitulate many aspects of a developing embryo. Of specific
importance, synchronous differentiation of EB recapitulates
organ-specific development and is achieved in culture by formation of
uniformly sized EB. The method described here demonstrates a simple and
cost-effective way of generating EB from murine ES cells. Single EB are
formed in a multi-well plate format and large numbers of EB are
generated using a 96-well multi-well plate. Uniform single-sized EB
formed in the multi-well are an ideal system for screening compounds
and determining differentiation effects. Since EB contain all three
germ layers, they are appropriate for studying small molecule effects
on differentiation of ES such as is performed in high-throughput
screening protocols
TECHNICAL NOTE - Single embryoid body formation in a multi-well plate
Embryoid bodies (EB) formed from murine embryonic stem (ES) cells
recapitulate many aspects of a developing embryo. Of specific
importance, synchronous differentiation of EB recapitulates
organ-specific development and is achieved in culture by formation of
uniformly sized EB. The method described here demonstrates a simple and
cost-effective way of generating EB from murine ES cells. Single EB are
formed in a multi-well plate format and large numbers of EB are
generated using a 96-well multi-well plate. Uniform single-sized EB
formed in the multi-well are an ideal system for screening compounds
and determining differentiation effects. Since EB contain all three
germ layers, they are appropriate for studying small molecule effects
on differentiation of ES such as is performed in high-throughput
screening protocols