14 research outputs found
Surface-Initiated Polymer Brushes in the Biomedical Field: Applications in Membrane Science, Biosensing, Cell Culture, Regenerative Medicine and Antibacterial Coatings
Antiadhesive polymer brush coating functionalized with antimicrobial and RGD peptides to reduce biofilm formation and enhance tissue integration
Antiadhesive Polymer Brush Coating Functionalized with Antimicrobial and RGD Peptides to Reduce Biofilm Formation and Enhance Tissue Integration
This paper describes the synthesis and characterization of polymer peptide conjugates to be used as infection-resistant coating for biomaterial implants and devices. Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP), able to kill bacteria on contact, and arginine glycine aspartate (RGD) peptides to promote the adhesion and spreading of host tissue cells. The antiadhesive and antibacterial properties of the coating were investigated with three bacterial strains: Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The ability of the coating to support mammalian cell growth was determined using human fibroblast cells. Coatings composed of the appropriate ratio of the functional components: PF127, PF127 modified with AMP, and PF127 modified with RGD showed good antiadhesive and bactericidal properties without hampering tissue compatibility
Antiadhesive Polymer Brush Coating Functionalized with Antimicrobial and RGD Peptides to Reduce Biofilm Formation and Enhance Tissue Integration
Antiadhesive Polymer Brush Coating Functionalized with Antimicrobial and RGD Peptides to Reduce Biofilm Formation and Enhance Tissue Integration
This
paper describes the synthesis and characterization of polymer–peptide
conjugates to be used as infection-resistant coating for biomaterial
implants and devices. Antiadhesive polymer brushes composed of block
copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial
peptides (AMP), able to kill bacteria on contact, and arginine–glycine–aspartate
(RGD) peptides to promote the adhesion and spreading of host tissue
cells. The antiadhesive and antibacterial properties of the coating
were investigated with three bacterial strains: Staphylococcus
aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The
ability of the coating to support mammalian cell growth was determined
using human fibroblast cells. Coatings composed of the appropriate
ratio of the functional components: PF127, PF127 modified with AMP,
and PF127 modified with RGD showed good antiadhesive and bactericidal
properties without hampering tissue compatibility