Concorrência tributária entre regiões da Rússia e seus indicadores

The article focuses on approaches to assessing the competitive advantages of regions of Russia in the area of taxation, the main approaches to the definition of tax competition at the present stage used in the scientific literature, are considered; emphasis on the influence of internal tax competition on social and economic development of the regions; the possibilities of using a number of quantitative and qualitative indicators to assess the competitive advantages of the regions in the area of taxation are summarized; ranking of the regions of Russia by using tax authorities to provide tax benefits of corporate income tax paid to the budget of the Russian Federation constituent, was performed; the conclusion about the relationship of investment activity and the availability of competitive advantages in the area of taxation was made; the development problems of the regional competitive advantages in the area of taxation and prospects for their solution are described.El artículo se centra en los enfoques para evaluar las ventajas competitivas de las regiones de Rusia en el área de la tributación, se consideran los principales enfoques para la definición de competencia fiscal en la etapa actual utilizados en la literatura científica; énfasis en la influencia de la competencia fiscal interna sobre el desarrollo social y económico de las regiones; se resumen las posibilidades de utilizar una serie de indicadores cuantitativos y cualitativos para evaluar las ventajas competitivas de las regiones en el área de la tributación; clasificación de las regiones de Rusia mediante el uso de las autoridades fiscales para proporcionar los beneficios fiscales del impuesto sobre la renta corporativo pagado al presupuesto del constituyente de la Federación de Rusia; se llegó a la conclusión sobre la relación de la actividad inversora y la disponibilidad de ventajas competitivas en el área de tributación; se describen los problemas de desarrollo de las ventajas competitivas regionales en el área de impuestos y las perspectivas para su solución. O artigo enfoca as abordagens para avaliar as vantagens competitivas das regiões russas na área de tributação, considerando as principais abordagens para a definição de concorrência tributária no atual estágio utilizado na literatura científica; ênfase na influência da concorrência fiscal interna no desenvolvimento social e econômico das regiões; as possibilidades de utilizar uma série de indicadores quantitativos e qualitativos para avaliar as vantagens competitivas das regiões na área de tributação são resumidas; classificação das regiões da Rússia, usando as autoridades fiscais para fornecer os benefíciosfiscais do imposto de renda corporativo pago ao orçamento do constituinte da Federação Russa; chegouse à conclusão sobre a relação entre a atividade de investimento e a disponibilidade de vantagens competitivas na área de tributação; São descritos os problemas de desenvolvimento de vantagenscompetitivas regionais na área tributária e as perspectivas de sua solução

Acid Sensitive Ion Channels as Target of Hydrogen Sulfide in Rat Trigeminal Neurons

© 2016, Springer Science+Business Media New York.Hydrogen sulfide (H2S), an endogenous gasotransmitter, was proposed to act as a signaling molecule in a wide variety of pathophysiological processes including inflammation and nociception. Acid-sensing ion channels (ASICs) are voltage-insensitive, proton-gated cation channels activated by extracellular acidosis, and are involved in various pathologies of the nervous system including ischemia, stroke, and migraine. In this study, the effect of the H2S donor-sodium hydrogen sulfide (NaHS) on the firing of trigeminal (TG) nerve was explored using suction electrode recordings in peripheral branches of the TG nerve in isolated rat meninges, and patch clamp recordings of ASIC currents in isolated TG neurons. NaHS (100 μM) increased the action potential frequency of TG nerve and bath application of NaHS increased the amplitude of ASIC currents triggered by focal application of low pH solution on isolated TG neurons. We propose that activation of ASICs by H2S during chronic inflammation process contributes to the increased excitability of the TG system and may be implicated in the generation of nociceptive firing underlying migraine pain

Multiplexed detection of cancer biomarkers using an optical biosensor

Early detection of cancer is important in administering timely treatment and increasing cancer survival rates. For early cancer detection one can use biomarkers, which are characteristics that can be objectively measured or evaluated as indicators of normal or pathogenic processes. In our study we study three protein biomarkers: carcinoembryonic antigen (CEA), interleukin-6 (IL-6) and extracellular protein kinase A (ECPKA), which have been implicated in various types of human cancer. The main objective of this project is to develop a biosensor for detection of multiple cancer biomarkers. To detect these protein biomarkers high affinity ssDNA aptamers are being selected. Aptamers are short single stranded DNAs with an ability to bind to various targets with high affinity and specificity which selected by SELEX (Systemic Evolution of Ligands through Exponential enrichment) [2]. Ultimately, aptamers against each of the biomarker will be conjugated to magnetic nanoparticles to capture biomarkers from biological fluids. Another aptamer is proposed to be conjugated to quantum dots for quantitation of biomarkers when analyzed on spectrometer

Multiplexed detection of cancer biomarkers using an optical biosensor

Early detection of cancer is important in administering timely treatment and increasing cancer survival rates. For early cancer detection one can use biomarkers, which are characteristics that can be objectively measured or evaluated as indicators of normal or pathogenic processes. In our study we study three protein biomarkers: carcinoembryonic antigen (CEA), interleukin-6 (IL-6) and extracellular protein kinase A (ECPKA), which have been implicated in various types of human cancer. The main objective of this project is to develop a biosensor for detection of multiple cancer biomarkers. To detect these protein biomarkers high affinity ssDNA aptamers are being selected. Aptamers are short single stranded DNAs with an ability to bind to various targets with high affinity and specificity which selected by SELEX (Systemic Evolution of Ligands through Exponential enrichment) [2]. Ultimately, aptamers against each of the biomarker will be conjugated to magnetic nanoparticles to capture biomarkers from biological fluids. Another aptamer is proposed to be conjugated to quantum dots for quantitation of biomarkers when analyzed on spectrometer

Cyclic voltammetry of tris(2,2-bipyridine)zinc(II) diperchlorate detected by electron spin resonance

Electrochemical transformations of the tris(2,2-bipyridine) complex of zinc(II) perchlor- ate were studied by cyclic voltammetry detected by electron spin resonance (DESR CV), which made it possible to indentify the intermediates formed and to monitor the unpaired electron localization in them. © 2013 Springer Science+Business Media, Inc

Case of the death due to drowning

Аналіз казуїстичних випадків смерті свідчить не лише про труднощі та особливості судово-медичних експертиз, але іноді не співпадає з танатогенезом від різних чинників смерті у воді, що може настати не лише від утоплення, але й від раптової зупинки серця, черепно-мозкової травми. Деякі автори виділяють рефлекторний тип утоплення пов’язаний з різким перепадом температур тіла людини і води

Effect of silica coating and further silica surface decoration by phospholipid bilayer on quenching of Tb(III) complexes by adrenochrome

© 2015 Elsevier B.V. Abstract The present report introduces regularities and mechanisms of Tb(III)-centered luminescence quenching by adrenochrome for Tb(III)-p-sulfonatothiacalix[4]arene complexes in the aqueous solutions and the same complexes doped into silica nanoparticles. The choice of adrenochrome (the oxidized form of adrenaline) as quencher originates from its quenching effect on the Tb(III)-centered luminescence, which discriminates it from adrenaline and dopamine. The quenching through dynamic mechanism of the Tb(III) complexes in the solutions results from their concentration induced collision with adrenochrome molecules. The quenching of the Tb(III)-doped silica nanoparticles also occurs through dynamic mechanism, although it is insignificant due to the shielding effect of the silica surface. The inclusion of the Tb(III)-doped silica nanoparticles into phospholipid bilayers influences the quenching of the Tb(III)-centered luminescence by adrenochrome due to its binding with the bilayers deposited onto silica nanoparticles

Receptor mechanisms mediating the pro-nociceptive action of hydrogen sulfide in rat trigeminal neurons and meningeal afferents

© 2017 Koroleva, Mustafina, Yakovlev, Hermann, Giniatullin and Sitdikova. Hydrogen sulfide (H 2 S), a well-established member of the gasotransmitter family, is involved in a variety of physiological functions, including pro-nociceptive action in the sensory system. Although several reports have shown that H 2 S activates sensory neurons, the molecular targets of H 2 S action in trigeminal (TG) nociception, implicated in migraine, remains controversial. In this study, using suction electrode recordings, we investigate the effect of the H 2 S donor, sodium hydrosulfide (NaHS), on nociceptive firing in rat meningeal TG nerve fibers. The effect of NaHS was also explored with p atch-clamp and calcium imaging techniques on isolated TG neurons. NaHS dramatically increased the nociceptive firing in TG nerve fibers. This effect was abolished by the TRPV1 inhibitor capsazepine but was partially prevented by the TRPA1 blocker HC 030031. In a fraction of isolated TG neurons, NaHS transiently increased amplitude of capsaicin-induced currents. Moreover, NaHS by itself induced inward currents in sensory neurons, which were abolished by the TRPV1 inhibitor capsazepine suggesting involvement of TRPV1 receptors. In contrast, the inhibitor of TRPA1 receptors HC 030031 did not prevent the NaHS-induced currents. Imaging of a large population of TG neurons revealed that NaHS induced calcium transients in 41% of tested neurons. Interestingly, this effect of NaHS in some neurons was inhibited by the TRPV1 antagonist capsazepine whereas in others it was sensitive to the TRPA1 blocker HC 030031. Our data suggest that both TRPV1 and TRPA1 receptors play a role in the pro-nociceptive action of NaHS in peripheral TG nerve endings in meninges and in somas of TG neurons. We propose that activation of TRPV1 and TRPA1 receptors by H 2 S during neuro-inflammation conditions contributes to the nociceptive firing in primary afferents underlying migraine pain

Anthocyanins as a factor in the alimentary restoration of cellular immunity in diet induced obesity in rats

The article presents the results of a study of the effect of anthocyanins on cellular immunity in rats on a model of alimentary obesity. The aim of the study was to study the effect of an anthocyanin- enriched diet on cellular immunity in diet induced obesity in rats. The study was carried out on male Wistar rats with an initial body weight of 108±2 g. The animals were randomized by body weight into 3 groups (8 pcs. in group). For 12 weeks, rats of the 1st (control) group received a complete modified diet of AIN93M; rats of the 2nd group consumed a high-calorie choline-deficient diet (HCChDD), the fat content of which was 45%, fructose – 20% of the energy value of the diet; rats of the 3rd group received HCChDD with the addition of standardized blueberry and blackcurrant extract (30% anthocyanins) at an average daily dose of 11 mg anthocyanins/kg body weight. The expression of differentiation markers of peripheral blood lymphocytes was carried out by flow cytofluorimetry. As a result of the study, it was found that in rats of the 2nd group with alimentary obesity, the relative content in the peripheral blood of T helpers (CD3+CD4+) was increased (p < 0.05) (75.75±1.11% versus 70.07±0 49% – group 1, 72.14±0.91% – group 3) and reduced (p < 0.05) content of T cytotoxic lymphocytes (CD3+CD8+) (22.54±1.14% versus 28.09±0.72% – 1st group, 26.07±0.87% – 3rd group). The CD3/CD4 ratio in rats of the 2nd group exceeded (p < 0.05) this index in rats of the 1st and 3rd groups (3.44±0.25 versus 2.47±0.09 – 1st group, 2.79±0.13 – 3rd group). Enrichment of the HCChDD with the blueberry and blackcurrant extract led to the normalization of these parameters of cellular immunity. The number of B lymphocytes (CD45R+), Т lymphocytes (CD3+) and NK cells (CD161+) in the rat peripheral blood of all experimental groups had no statistically significant differences. The results of the study of cellular immunity in rats with alimentary obesity indicate the presence of metainflammation. The received data indicate the prospect of using biologically active substances

Spectroelectrochemistry: ESR of Paramagnetic Intermediates in the Electron Transfer Series [Cr(bpy)<inf>3</inf>]ⁿ (n = 3+, 2+, 1+, 0, 1-)

© 2015 Elsevier Ltd. All rights reserved. A new method of cyclic voltammetry with simultaneous ESR spectra multiple recording (CV-MRESR) has been proposed and implemented. Along with cyclic voltammetry (CV) and electron spin resonance (ESR), the combined method involving cyclic voltammetry and ESR (CV-ESR) has been used for the study of electron transfer in a series of complexes [Cr(bpy)3]n (n = 3+, 2+, 1+, 0, 1-)