Differential ESAT6-induced IFNγ and CXCL9 in pulmonary and extrapulmonary TB.

<p>Whole blood cells were stimulated with ESAT6 at 5 µg/ml, and IFNγ measured in cell supernatants harvested at 5 day post-stimulation, while CXCL9 was measured at 2 days post-stimulation. TB patient groups comprised those with pulmonary TB (PTB, n = 30), less severe EPulTB (LNTB, n = 24) and severe EPulTB (SevTB, n = 22). The box plots represent data from each group after cytokine secretion from unstimulated cells has been subtracted. The whiskers indicate the 25^th and 75^th quartile respectively, while the median line separates the two. ‘*’ denotes significant differences between groups, p<0.05; (*). A. IFNγ, B. CXCL9.</p

Differential LPS-induced CXCL9 and CCL2 in limited and severe TB.

<p>Whole blood cells were stimulated with LPS at 1 µg/ml and CXCL9 measured in cell supernatants harvested at 2 days post-stimulation. All other parameters as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005158#pone-0005158-g001" target="_blank">Fig. 1</a>. A. CXCL9 and B. CCL2.</p

ESAT6- and CFP10-induced cytokine and chemokine responses.

<p>EC, healthy endemic controls; TB, tuberculosis patients.</p><p>‘δ’ denotes cytokine secretion after background subtraction in each case.</p>*<p>denotes significant difference (p<0.05) as compared with EC.</p><p>All statistical analyses performed using the Mann-Whitney U non-parametric test.</p