5 research outputs found
Protein spots of kidney preservation solution identified by 2D gel electrophoresis.
<p>Protein spots of kidney preservation solution identified by 2D gel electrophoresis.</p
List of proteins and peptides that were isolated from kidney preservation solution which showed a statistically significant correlation with donors’ age (DA), cold ischemia time (CIT), creatinine (Cr) and blood urea nitrogen (BUN).
<p>List of proteins and peptides that were isolated from kidney preservation solution which showed a statistically significant correlation with donors’ age (DA), cold ischemia time (CIT), creatinine (Cr) and blood urea nitrogen (BUN).</p
Proteins and peptides belonging to kidney tissues detected from preservation solution using LC-MS/MS (n:18).
<p>Proteins and peptides belonging to kidney tissues detected from preservation solution using LC-MS/MS (n:18).</p
International Comparison of Enumeration-Based Quantification of DNA Copy-Concentration Using Flow Cytometric Counting and Digital Polymerase Chain Reaction
Enumeration-based
determination of DNA copy-concentration was assessed
through an international comparison among national metrology institutes
(NMIs) and designated institutes (DIs). Enumeration-based quantification
does not require a calibration standard thereby providing a route
to “absolute quantification”, which offers the potential
for reliable value assignments of DNA reference materials, and International
System of Units (SI) traceability to copy number 1 through accurate
counting. In this study, 2 enumeration-based methods, flow cytometric
(FCM) counting and the digital polymerase chain reaction (dPCR), were
compared to quantify a solution of the pBR322 plasmid at a concentration
of several thousand copies per microliter. In addition, 2 orthogonal
chemical-analysis methods based on nucleotide quantification, isotope-dilution
mass spectrometry (IDMS) and capillary electrophoresis (CE) were applied
to quantify a more concentrated solution of the plasmid. Although
9 dPCR results from 8 laboratories showed some dispersion (relative
standard deviation [RSD] = 11.8%), their means were closely aligned
with those of the FCM-based counting method and the orthogonal chemical-analysis
methods, corrected for gravimetric dilution factors. Using the means
of dPCR results, the RSD of all 4 methods was 1.8%, which strongly
supported the validity of the recent enumeration approaches. Despite
a good overall agreement, the individual dPCR results were not sufficiently
covered by the reported measurement uncertainties. These findings suggest
that some laboratories may not have considered all factors contributing
to the measurement uncertainty of dPCR, and further investigation
of this possibility is warranted
Additional file 2: of The use of digital PCR to improve the application of quantitative molecular diagnostic methods for tuberculosis
Protocol for gDNA extraction and dPCR quantification of materials. (PDF 394 kb