RADIATION CROSSLINKED ADVANCED WOUND DRESSING CONTAINING EGG WHITE PROTEINS

During the past decades, the use of antibiotics has grown worldwide in different fields, from medicine to agriculture, leading to antibiotic resistance of microbes, which is the cause of thousands of deaths every year worldwide. [1, 2] Finding new antibiotics is becoming more and more difficult because their development is no longer convenient in term of the cost–benefit ratio for the pharmaceutical industry. The interest in egg white proteins, such as ovoalbumin, ovotransferrin and lysozyme, has risen especially because of their demonstrated antimicrobial activities. [3] These antimicrobial proteins can then be used to enrich wound dressing films that requires a high level of control of microbial colonization. Wound dressing films can be easily produced crosslinking polymers, such as polyvinyl alcohol (PVA) or polyvinyl pyrrolidone, by high-energy radiation. [4] This technique does not require expensive initiators and catalysts and, depending on the irradiation doses, it can guarantee simultaneous product sterilization. PVA hydrogels obtained by irradiation are also transparent, a desirable property for wound dressings. When PVA is mixed with selected polysaccharides, the dressings have shown faster healing rates and scarless healing, probably due to antioxidant properties of polysaccharide fragments produced upon irradiation. [4] Therefore, the aim of this work is to develop hydrogel would dressings based on the irradiation of PVA and xyloglucan (XG) blends and to investigate the antibacterial properties of the films due to the incorporation of egg white proteins

Injectable xyloglucan hydrogels incorporating spheroids of adipose stem cells for bone and cartilage regeneration

Cartilage or bone regeneration approaches based on the direct injection of mesenchymal stem cells (MSCs) at the lesion site encounter several challenges, related to uncontrolled cell spreading and differentiation, reduced cell viability and poor engrafting. This work presents a simple and versatile strategy based on the synergic combination of in-situ forming hydrogels and spheroids of adipose stem cells (SASCs) with great potential for minimally invasive regenerative interventions aimed to threat bone and cartilage defects. Aqueous dispersions of partially degalactosylated xyloglucan (dXG) are mixed with SASCs derived from liposuction and either a chondroinductive or an osteoinductive medium. The dispersions rapidly set into hydrogels when temperature is brought to 37 °C. The physico-chemical and mechanical properties of the hydrogels are controlled by polymer concentration. The hydrogels, during 21 day incubation at 37 °C, undergo significant structural rearrangements that support cell proliferation and spreading. In formulations containing 1%w dXG cell viability increases up to 300% for SASCs-derived osteoblasts and up to 1000% for SASCs-derived chondrocytes if compared with control 2D cultures. The successful differentiation into the target cells is supported by the expression of lineage-specific genes. Cell-cell and cell-matrix interactions are also investigated. All formulations resulted injectable, and the incorporated cells are fully viable after injection

Hydrogel scaffolds blends to host Spheroids from human adipose stem cells

INTRODUCTION Adipose stem cells represent a reliable source of stem cells for their widely demonstrated potential in regenerative medicine and tissue engineering applications. New recent insights show that 3D models may properly mimic the native tissue properties; in fact Spheroids from Adipose derived Stem Cells (S-ASCs) displayed enhanced regenerative abilities if compared to 2D models. Stem cell therapy success is determined by "cell-quality" thus the involvement of stress signals and cellular aging need to be deeply investigated. The development of 3D cell-laden hydrogels has enabled to mimic the peculiar scenario of a native tissue. We studied SASCs-cell quality and tested their viability and differentiation abilities in new hydrogels. METHODS S-ASCs were obtained from liposuction of healthy patients. Analysis of aging, telomeric length and stress-oxidative genes was performed through Real-Time PCR. Physico-chemical, morphological and mechanical properties of k-Carrageenan (k-C, 2%w) and degalactosylated xyloglucan (Deg-XG, 2%w) hydrogels were defined. S-ASCs compatibility with hydrogels was evaluated by viability test and mesenchymal differentiation abilities. RESULTS Gene expression of genes linked with stemness, senescence and stress-oxidative was evaluated and correlated with SASCs-cell quality. Indeed, aging-related p16INK4a mRNA is downregulated while anti-aging Sirtuin1 is upregulated in 3D-SASCs. Furthermore, vegetal-origin hydrogels have guaranteed an optimal environment for S-ASCs in stemness and mesenchymal differentiation conditions. CONCLUSION Bio-instructive scaffolds are critical for exploiting stem cells therapeutic potential in tissue engineering. This study provides a versatile approach to investigate the interactions between cells in controlled settings, opening up novel 3D in vitro approaches to mimic the tissues complexity

Moringa oleifera leaf powder as functional additive in cookies to protect sh-sy5y cells

The aim of this work is the evaluation of the addition of Moringa leaf powder (MLP) in cookies in terms of antioxidant properties, dough processability and sensorial properties of the cookies. The total content of biophenols and flavonoids in MLP was detected and the identification of the bioactive molecules was performed by HPLC-ESI-TOF-MS measurements, before and after oven treatment at 180◦C for 20 min. After a preliminary evaluation of the MLP water soluble fraction (MLPsf) cytotoxicity, its protective effect against an oxidative injury induced in the SH-SY5Y cells was assessed. Data evidence that the bioactive molecules present in MLPsf are effective in preventing ROS production and in protecting neuronal cells against oxidative stress. Prototypes of cookies containing MLP in different concentrations were then produced and evaluated by a consumer panel. Selected doughs containing MLP were analysed to determine the total content of biophenols in the cookies after baking and their enrichment in terms of valuable chemical elements. The influence of MLP on the viscoelastic behaviour and morphology of the doughs was also assessed. Finally, the potential role in counteracting the insurgence of not treatable neurodegenerative pathologies of two main MLP components, glucomoringin and kaempferol derivatives, present also after the thermal treatment, was discussed

Intercropping of tomato with antagonistic plants in the treatment of soil infested with Meloidogyne spp., under greenhouse

El intercultivo de tomate con especies antagónicas se considera una alternativa de manejo de Meloidogyne spp. El objetivo del trabajo fue evaluar el intercultivo de las especies Tagetes erecta, Tagetes minuta y Crotalaria juncea para el manejo de Meloidogyne spp., en el cultivo de tomate. Se determinó, en las plantas de tomate, el índice de agallas y el número y peso de frutos. El intercultivo de tomate con T. erecta mostro un menor índice de agallas que el resto de los tratamientos evaluados. Además, se observó en este tratamiento, y en el de T. minuta, un mayor peso y número de frutos con respecto al tratamiento con C. juncea y al testigo sin intercultivo. En las condiciones de este ensayo, el intercultivo de tomate con T. erecta mostró ser una buena alternativa de manejo de Meloidoigyne spp. Se contribuye con esta práctica a una menor utilización de fitosanitarios, con el efecto positivo que trae aparejado para con el medio ambiente, y a la obtención de un producto hortícola de mayor inocuidad para el consumo.Tomato intercropping with antagonistic species is considered a management alternative for Meloidogyne spp. The objective of the work was to evaluate the intercropping of Tagetes erecta, Tagetes minuta and Crotalaria juncea for the management of Meloidogyne spp., in tomato cultivation. The gall index and the number and weight of fruits were determined in tomato plants. Tomato intercropping with T. erecta showed a lower gall index than the rest of evaluated treatments. In addition, a higher weight and number of fruits were observed in this treatment and in the one with T. minuta, respect to the C. juncea treatment and the control without intercropping. Under the conditions of this trial, intercropping of tomato with T. erecta proved to be a good alternative for the management of Meloidoigyne spp. This practice contributes to a lesser use of phytosanitary products, with the positive effect that it has on the environment, and to the obtaining of a more innocuous horticultural product for human consumption.Fil: Comezaña, María Micaela. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca; ArgentinaFil: Rodríguez, R. A.. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Ayastuy, M. E.. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Muscolino, C.. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Rosetti, F.. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Belladonna, D. P.. Universidad Nacional del Sur. Departamento de Agronomía; Argentin

La sinistra:italiana una commedia in tre atti.

L’entrata nella seconda Repubblica è stata scandita dalla marcia trionfale della sinistra in predicato di vincere le elezioni politiche. I risultati elettorali più gratificanti della sinistra sono stati la traballante vittoria del 1996 e le non vittorie (sostanziali pareggi) del 2006 e del 2013, al contrario erano certe nelle previsioni e nei fatti le vittorie berlusconiane del 2001 e del 2008

Prognostic Factors in 77 Curative Chest Wall Resections for Isolated Breast Cancer Recurrence

Background: Full-thickness chest wall resection (CWR) is the preferred treatment for breast cancer (BC) patients with extensive isolated locoregional recurrence. It remains a challenge to select patients that will benefit most from this treatment. The aim of this study was to define prognostic factors in patients who undergo CWR with curative intent. Methods: BC patients who underwent a CWR with curative intent for recurrence of disease between 1986 and 2006 were included in this retrospective study. Twenty-two factors were studied in a univariate analyses, and multivariate stepwise Cox regression analyses was performed. Results: Seventy-seven patients were included in this study. The 5-year overall survival was 25%. There was one postoperative death. Univariate analyses showed that three prognostic factors were significantly correlated with OS and disease-free survival: (1) interval between primary treatment and CWR (P = .02 and .004, respectively), (2) chemotherapy for recurrence (P = .05 and .05, respectively), and (3) resection specimen smaller than 150 cm2(P = .03 and .009, respectively). An interval lasting >10 years between primary treatment and CWR remained statistically significantly correlated with better overall survival and disease-free survival after multivariate analyses. Conclusions: CWR is a safe treatment in patients who have isolated extensive BC recurrence. The best survival outcome was seen in patients after a disease-free interval of >10 years. Existing data show that adjuvant radiotherapy and adjuvant hormone therapy for estrogen-positive tumors improves overall survival. Neoadjuvant chemotherapy may be considered in individual patients