4,281 research outputs found
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Whither Genomics?
The flood of data from genome-wide analysis is transforming biology. We need to develop new, interdisciplinary approaches to convert these data into information about the components and structures of individual biological pathways and to use the resulting information to yield knowledge about general principles that explain the functions and evolution of life.Molecular and Cellular Biolog
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When it comes to teaching and tenure it is time to walk the walk.
Institutions should value teaching and service, and not just research, when considering faculty for promotion and tenure
Design of a processor to support the teaching of computer systems
Teaching computer systems, including computer architecture, assembly language programming and operating system implementation, is a challenging occupation. At the University of Waikato this is made doubly true because we require all computer science and information systems students study this material at second year. The challenges of teaching difficult material to a wide range of students have driven us to find ways of making the material more accessible. The corner stone of our strategy for delivering this material is the design and implementation of a custom CPU that meets the needs of teaching. This paper describes our motivation and these needs. We present the CPU and board design and describe the implementation of the CPU in an FPGA. The paper also includes some reflections on the use of a real CPU rather than a simulation environment. We conclude with a discussion of how the CPU can be used for advanced classes in computer architecture and a description of the current status of the project
So, where is queer? A critical geography of queer exhibitions in Australia
This paper interrogates the geography of queer exhibitions in museums and galleries in Australia. The analysis draws on data from Museums Australia\u27s database of queer exhibitions (1982-2005), which are cross-tabulated with geographical variables such as location, scale and state/territory population. The findings show an uneven geographical distribution of exhibitions, how geography also frames the themes of queer exhibitions, and an imbalanced geography, in which regional histories are few, national and state scale histories are prevalent, and minimal exhibitions occur outside metropolitan areas. This is problematic because queer identities, communities and histories vary across scales and between places. Appreciation of geography is thus useful for developing policies and practices that ensure the diversity of queer communities and histories is represented and communicated in exhibitions
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Complications Dawn for Kinetochore Regulation by Aurora
Organisms must faithfully segregate their chromosomes during cell division; mistakes in this process can be costly and even fatal to the organism (1, 2). During mitosis, replicated chromosomes attach to the spindle, a dynamic system of microtubules organized around two poles. Chromosomes attach to the spindle via kinetochores, structures that form on centromeres and bind the ends of microtubules. For accurate segregation, kinetochores on sister chromosomes must attach to microtubules from opposite poles; incorrect attachments lead to missegregation (3). In PNAS, Umbreit et al. (4) expand our understanding of how kinetochoreāmicrotubule interactions can be regulated to correct improper attachments. The authors use in vitro studies to demonstrate that a component of the kinetochore, the Ndc80 complex, can directly influence the dynamics of the microtubules it is bound to and how the complex can be regulated to correct errors in chromosome attachment.
Kinetochores are complicated machines. They can stay attached to microtubule ends as they grow and shrink, regulate the dynamics of microtubules, regulate their own activity, and signal to the remainder of the cell. The outer layer of the kinetochore contains the dumbbell-shaped Ndc80 complex (5): One globular domain [the N-terminal domains of Hec1 (Ndc80 in budding yeast) and Nuf2] binds microtubules (6) and is connected by a long coiled coil to the other globular domain (composed of the C-terminal domains of Spc24/Spc25), which connects to other kinetochore components (7) (Fig. 1A). Hec1 contains a conserved calponin homology domain and an unstructured N-terminal tail: Both regions can bind to microtubules independently, but they must act together to produce high-affinity binding (5āāā8). When sister kinetochores attach...(see full text).Molecular and Cellular Biolog
Decreased Accumulation of Cyclic Adenosine 3ā²,5ā²-Monophosphate in āIschemicā Skin after 12-0-Tetradecanoyl-phorbol-13-acetate Treatment
The effect of 12-0-tetradecanoyl-phorbol-13-acetate (TPA) on cyclic adenosine 3ā²,5ā²-monophosphate (cyclic AMP) level in adult mouse skin in response to ischemia was examined. The incubation of skin pieces in a buffered salts medium at 37Ā°C resulted in a rapid accumulation of cyclic AMP. In mouse skin pieces maximum accumulation (about 6times the basal level) occurred after 2min incubation and was followed by a rapid decline in the cyclic AMP level. This āischemicā rise in epidermal cyclic AMP was greatly reduced if skin was used 16hr after a single application of 17nmoles of TPA. The effect of TPA on cyclic AMP accumulation in response to ischemia was first observed at 1hr after TPA treatment and was maximal at 4hr. The lack of āischemicā response in TPA-treated skin was not related to an increase in the activity of cyclic AMP phosphodiesterase after TPA application. In addition, the accumulation of cyclic AMP in skin in response to both ischemia and exposure to isoproterenol, adenosine, histamine, or prostaglandin E2(PGE2) was not observed in skin treated with the tumor promoter TPA
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