Additional file 1: Figure S1. of MiRNA-146b-5p upregulates migration and invasion of different Papillary Thyroid Carcinoma cells

Overexpression of miR-146b-5p increases migration and invasion of the PTC cell lines, TPC-1 and BCPAP. Cells were transfected with an oligonucleotide mimics miR-146b-5p (miR-146b) (50nM), as described in the Methods section. Three control groups were used: (1) cells cultured in regular culture medium (identified as TPC-1, BCPAP), (2) cells incubated with the transfection agent only (Mock) and (3) cells transfected with a negative miR-control (Neg). Forty-eight hours after transfection miR-146b-5p expression (A, D) was evaluated. Transwell migration (without basement membrane) and invasion (with basement membrane) assays were performed during 8 h. Quantitative data are shown for migration (B, E) and invasion assays (C, F). All experiments were performed three times. TPC-1/BCPAP: cell, Mock: cell + transfection agent, Neg: cell + mimics-miR negative control, miR-146b: cell + mimics miR-146b-5p. Statistically significant differences: * P < 0,01 (TPC-1/BCPAP versus miR-146b); ** P < 0,01 (Mock versus miR-146b), *** P > 0,01 (Neg versus miR-146b). (TIF 521 kb

Additional file 3: Figure S3. of MiRNA-146b-5p upregulates migration and invasion of different Papillary Thyroid Carcinoma cells

Overexpression of miR-146b-5p in TPC-1 and BCPAP cells does not affect morphology and F-actin distribution. Forty hours after transfection, cells were seeded upon glass coverslips (18 mm) without and with Matrigel® coating (10 μg/ml) and cultured for 8 h. After this period, cells were fixed, stained for F-actin (green) and nucleus (blue) and analyzed by confocal microscopy. Representative images of TPC-1 and BCPAP cells are shown. Cells are polarized and show one or two predominant lamellipodia. TPC-1/BCPAP: cell, Mock: cell + transfection agent, Neg: cell + mimics-miR negative control, miR-146b: cell + mimics-miR-146b. Bars: 10 μm. (TIF 1884 kb

Mère et ses enfants (Laponie), Frankl : [photographie de presse] / [Agence Rol]

Référence bibliographique : Rol, 99758Appartient à l’ensemble documentaire : Pho20RolImage de press

Additional file 4: Table S2. of Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth

Correlation of the differentially expressed genes identified in a knock down of nc886 in esophageal, gastric and thyroid cell lines and nc886 TSS200 methylation level in TCGA-PRAD tumor samples. (XLSX 11 kb

Additional file 3: Table S1. of Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth

Correlation of the expression of the DNMTs with nc886 TSS200 methylation level in TCGA-PRAD tumor samples. (XLSX 9 kb

Additional file 1: Figure S1. of Nc886 is epigenetically repressed in prostate cancer and acts as a tumor suppressor through the inhibition of cell growth

Hierarchical cluster based on nc886 methylation status of paired normal and tumor tissue of PrCa patients from TCGA-PRAD and Stanford datasets. Clusterization was performed by MEV [42] using Euclidean algorithm with default parameters. The beta-values of methylation of the 6 CpG sites comprise the TSS200 in the normal and tumor tissue considered as a unit. Each row corresponds to one patient whose identity is indicated at the right of the heatmap using the ID provided by the original study. Upper rulers indicate the amplitude of gene expression represented by the colors of the heatmap. (PDF 147 kb