The protective effects of omega-3 fatty acids on rat testicular tissue

Objectives: In this study, the protective effect of omega-3 fatty acids on testicular tissue was aimed to investigate at biochemical levels.Materials and methods: Totally, 16 adult male Wistar rats were divided into two groups. Rats in Group I were used as control and only saline was given by intragastric gavage. Rats in Group II, 400 mg/kg dose ω-3 fatty acids were given daily by intragastric gavage. At the end of the six-week experimental period, all rats were killed by decapitation. The testicular tissue specimens taken from animals, superoxide dismutase, glutathione peroxidase, malondialdehyde, enzyme activities were measured spectrophotometrically. In addition, blood testosterone levels were examined.Results: In our study, ω-3 fatty acids in rats were given a statistically significant increase in the levels of superoxide dismutase, and glutathione peroxidase a statistically significant decrease in malondialdehyde levels were determined when compared to control group. In addition, ω-3 fatty acids in rats given a statistically significant increase in blood testosterone levels were observed.Conclusion: We concluded that ω-3 fatty acid had favorable effects in rat testis tissue by preventing oxidative damage and increasing the level of testosterone

Sıçanlarda Formaldehit Maruziyetiyle Testislerde Oluşan Morfolojik Değişiklikler Üzerine Melatonin Hormonunun Koruyucu Etkisi

Çalışmamızda, formaldehitin testis dokusu üzerine olan toksik etkileri ve bu toksik etkilere karsı melatonin hormonunun koruyucu etkisi biyokimyasal ve immunohistokimyasal düzeylerde araştırıldı. Bu amaçla, 21 adet Wistar-Albino cinsi erkek sıçan üç gruba ayrıldı. Grup I’deki sıçanlar kontrol olarak kullanıldı. Grup II’deki sıçanlara gün aşırı olarak formaldehit enjekte edildi. Grup III’deki sıçanlara ise formaldehit enjeksiyonu ile birlikte melatonin uygulandı. Bir aylık deney süresi sonunda, bütün sıçanlar dekapitasyon yöntemi ile öldürüldü. Daha sonra, sıçanların testisleri çıkartılarak çevre dokulardan ayrıldı. Testis doku örneklerinin bir kısmında süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px) enzim aktiviteleri ile malondialdehit (MDA) seviyesi spektrofotometrik olarak belirlendi. Testis doku örneklerinin bir bölümü ise immunohistokimyasal incelemeler için kullanıldı. Formaldehit uygulanan sıçanlarda SOD ve GSH-Px aktivitelerinin kontrol grubuna göre anlamlı bir şekilde azaldığı, MDA düzeylerinin ise yine istatistiksel olarak anlamlı bir şekilde arttığı tespit edildi. Ayrıca formaldehit maruziyeti sonrası testis dokusunda apoptotik değişikliklerin meydana geldiği immunohistokimyasal yöntemlerle belirlendi. Formaldehit maruziyeti ile birlikte melatonin enjekte edilen sıçanlarda ise SOD ve GSH-Px enzim aktivitelerinde bir artış olurken, MDA değerlerinde istatistiksel olarak anlamlı bir düsüs oldugu görüldü. Üstelik bu grupta, formaldehit maruziyeti sonucu oluşan apoptotik değişikliklerin gerilediği tespit edildi. Sonuç olarak, formaldehit maruziyetine bağlı olarak testis dokusunda oluşan oksidatif hasarın ve apoptozisin melatonin uygulamasıyla baskılandığı belirlendi.In our study, toxic effects of formaldehyde on testicular tissue and protective effects of melatonin hormone against these toxic effects were investigated at biochemical and immunohistochemical levels. For this purpose, 21 male Wistar-Albino rats were divided into three groups. Rats in group I were used as control. Rats in group II were injected every other day with formaldehyde. Rats in group III were administered melatonin with injection of formaldehyde. At the end of one month experimental period, all rats were killed by decapitation. Then the testes of rats were removed and dissected from the surrounding tissue. The activites of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the levels of malondialdehyde (MDA) were determined in the some of testicular tissue specimens by using spectrophotometric methods. The remaining testicular tissue specimens were used for immunohistochemical examination. The activities of SOD and GSH-Px were significantly decreased, and MDA levels were significantly increased in rats treated with formaldehyde compared to control. Additionally, apoptotic changes were occurred in testicular tissue after exposure of formaldehyde. It was seen that increase of SOD and GSHPx enzyme activities and decrease of MDA levels in rats administered melatonin with exposure of formaldehyde. Furthermore, apoptotic changes caused by formaldehyde were regressed in this group. In conclusion, it was determined that oxidative damage and apoptosis in testicular tissue caused by exposure of formaldehyde were suppressed by administration of melatonin