Cross-sectional seroprevalence surveys of SARS-CoV-2 antibodies in children in Germany, June 2020 to May 2021

The rate of SARS-CoV-2 infections in children remains unclear due to many asymptomatic cases. We present a study of cross-sectional seroprevalence surveys of anti-SARS-CoV-2 IgG in 10,358 children recruited in paediatric hospitals across Germany from June 2020 to May 2021. Seropositivity increased from 2.0% (95% CI 1.6, 2.5) to 10.8% (95% CI 8.7, 12.9) in March 2021 with little change up to May 2021. Rates increased by migrant background (2.8%, 4.4% and 7.8% for no, one and two parents born outside Germany). Children under three were initially 3.6 (95% CI 2.3, 5.7) times more likely to be seropositive with levels equalising later. The ratio of seropositive cases per recalled infection decreased from 8.6 to 2.8. Since seropositivity exceeds the rate of recalled infections considerably, serologic testing may provide a more valid estimate of infections, which is required to assess both the spread and the risk for severe outcomes of SARS-CoV-2 infections

LuMPIS - A modified luminescence-based mammalian interactome mapping pull-down assay for the investigation of protein-protein interactions encoded by GC-low ORFs

The GC content is highly variable among the genomes of different organisms. It has been shown that recombinant gene expression in mammalian cells is much more efficient when GC-rich coding sequences of a certain protein are used. In order to study protein-protein interactions in Varicella zoster virus, a GC-low herpesvirus, we have developed a novel luminescence-based maltose-binding protein pull-down interaction screening system (LuMPIS) that is able to overcome the impaired protein expression levels of GC-low ORFs in mammalian expression systems. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.Fil: Vizoso Pinto, María Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ludwig Maximilians Universitat; AlemaniaFil: Villegas, Josefina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Ludwig Maximilians Universitat; AlemaniaFil: Peter, Jan. Ludwig Maximilians Universitat; AlemaniaFil: Haase, Rudolf. Ludwig Maximilians Universitat; AlemaniaFil: Haas, Jürgen. Ludwig Maximilians Universitat; Alemania. University of Edinburgh; Reino UnidoFil: Lotz, Amelie Sophia. Ludwig Maximilians Universitat; AlemaniaFil: Muntau, Ania Carolina. Ludwig Maximilians Universitat; AlemaniaFil: Baiker, Armin. Ludwig Maximilians Universitat; Alemani