Boron extraction and vertical mobility in Paraná State oxisol, Brazil.

The deficiency or excess of micronutrients has been determined by analyses of soil and plant tissue. In Brazil, the lack of studies that would define and standardize extraction and determination methods, as well as lack of correlation and calibration studies, makes it difficult to establish limits of concentration classes for analysis interpretation and fertilizer recommendations for crops. A specific extractor for soil analysis is sometimes chosen due to the ease of use in the laboratory and not in view of its efficiency in determining a bioavailable nutrient. The objectives of this study were to: (a) evaluate B concentrations in the soil as related to the fertilizer rate, soil depth and extractor; (b) verify the nutrient movement in the soil profile; (c) evaluate efficiency of Hot Water, Mehlich-1 and Mehlich-3 as available B extractors, using sunflower as test plant. The experimental design consisted of complete randomized blocks with four replications and treatments of five B rates (0, 2, 4, 6, and 8 kg ha-1) applied to the soil surface and evaluated at six depths (0?0.05, 0.05?0.10, 0.10?0.15, 0.15?0.20, 0.20?0.30, and 0.30?0.40 m). Boron concentrations in the soil extracted by Hot Water, Mehlich-1 and Mehlich-3 extractors increased linearly in relation to B rates at all depths evaluated, indicatingB mobility in the profile. The extractors had different B extraction capacities, but were all efficient to evaluate bioavailability of the nutrient to sunflower.

Effect of increasing doses of glyphosate on water use efficiency and photosynthesis in glyphosate-resistant soybeans.

The increase cultivated area of GR soybeans across different regions of the world have raised questions not yet answered as related secondary effects of glyphosate on physiology of GR soybeans. In this work, we attempted to measure the effect of increasing glyphosate doses on water absorption and photosynthetic parameters of soybean, cv. BRS 242 GR. Plants were grown in completed nutrient solution (Hoagland & Arnon, 1950) in a greenhouse equipped with an evaporative cooling system (25?35 : 20?22C day/night) under natural daylight. The commercially formulated isopropylamine salts of glyphosate was sprayed on foliar doses of 450, 675, 900, 1350 and 1800 g. e.a ha-1 either in single application at four leaf stage (24 DAE) and in sequential application (24 and 36 DAE) (50%-50%) at four and five leaf stages, respectively. Using an infrared gas analyzer (IRGA), fluorometer (pulse-modulated) and chlorophylometer, before and after herbicide application net photosynthesis (A), transpiration rate (E), stomatal conductance (gs), sub-stomatal CO2 (Ci), carboxilation efficiency (A/Ci), fluorescence (Fs), maximal fluorescence (Fms); yield of quantum efficiency (Y=Fm-Fs/Fms) and chlorophyll contents were monitored. In addition, water absorption was measured daily and biomass yield and water use efficiency (WUE) were estimated by harvesting plants at R1 stage. All measures of photosynthetic parameters (A, E, gs, Ci) and Fs, Fms and Y were affected by increasing glyphosate doses. Chlorophyll contents were reduced right after glyphosate use, however with decreased in chlorophyll content and carboxilation efficiency, (A/Ci) the values suggest that glyphosate might have interfering, in some way, in the synthesis of chlorophyll and/or in carboxilative metabolism of photosynthesis (Calvin cycle), which contributed to dry biomass reductions. Total amount of water absorbed and biomass production by plants were also decreased as glyphosate doses increased, with a more intense effect of single application, as compared to sequential. WUE was also significantly reduced with increasing glyphosate doses. Under single and sequential application, soybean plants need 13 to 20% and 8 to 14% more water to produce the same amount of biomass, respectively

Induction, growth kinetics and morpho-histological characterization of Neem callus.

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Avaliação da atividade antimicrobiana de extrato aquoso e etanólico de Acanthospermum australe.

As plantas medicinais têm sido amplamente empregadas na terapia de diversas patologias, constituindo parte das ferramentas terapêuticas utilizadas no controle das mais variadas moléstias humanas. A atividade antibacteriana dos extratos aquoso e etanólico de Acanthospermum australe (Loefl.) O. Kuntze foi avaliada frente às bactérias Staphylococcus aureus (ATCC 6538P) e Escherichia coli (ATCC 25922), pelo método de difusão em Agar. Foi observada a inibição de crescimento destes microrganismos por ambos os extratos, nas concentrações testadas. Paralelamente, foi realizada a análise fitoquímica dos extratos aquoso e hidroetanólico para a determinação de compostos fenólicos (taninos, flavonoides, ácidos fenólicos e antraquinonas), alcaloides e compostos terpênicos. Os extratos apresentaram perfis qualitativamente semelhantes, apresentando taninos, flavonoides, ácidos fenólicos e compostos terpênicos

Utilização de materiais orgânicos no manejo da casca preta do inhame.

O objetivo do presente trabalho foi avaliar a influência da incorporação de materiais orgânicos ao solo sobre a densidade populacional dos nematoides causadores da doença