PENGARUH PENERAPAN METODE BERMAIN PERAN (ROLE PLAYING) PADA PEMBELAJARAN AL-QUR’AN HADITS TERHADAP HASIL BELAJAR PESERTA DIDIK KELAS IV MI AL- KARIMAH TANRUNG KECAMATAN AJANGALE KABUPATEN BONE

This study is a quasi-experimental design research that aims to determine the application of the role playing method in learning Al-Qur'an Hadith in class IV MI Al-Karimah Tanrung Kec.Ajangale Kab.Bone, to determine student learning outcomes before and after the role playingmethod was applied to the learning of the Qur'an Hadith in class IV MI Al-Karimah Tanrung Kec.Ajangale Kab.Bone, and to find out the effect of the application of the role playing method on learning the Qur'an 'an Hadith on the learning outcomes of fourth grade students of MI Al-Karimah Tanrung Kec.Ajangale Kab.Bone. The sample taken in this study amounted to 12 students using data collection techniques, namely tests, observations and documentation. Data analysis using simple regression with the help of SPSS 25.0 application. Based on the results ofthe descriptive analysis of the learning outcomes of fourth grade students at MI Al-Karimah Tanrung Kec. Ajangale Kab. Bone average value of Post Test after being processed into SPSS 25.0 is 82.08 and the average value of Pre-test is 64.17. There is an influence of the role playingmethod on learning Al-Qur'an Hadith on the learning outcomes of fourth grade students of MI Al-Karimah Tanrung Kec.Ajangale Kab.Bone. This can be seen in the t-test analysis that has been carried out using simple regression using SPSS showing Thit = 5,750 with Ttable = 2,262. The significance value obtained is smaller than (0.000 > 0.05). With the magnitude of the influence of 76.8% of the test results of the coefficient of determination (R Square).Penelitian ini merupakan penelitian quasi experimental desain yang bertujuan untuk untuk mengetahui penerapan metode bermain peran (role playing) pada pembelajaran Al-Qur’an Hadits di kelas IV MI Al-Karimah Tanrung Kec.Ajangale Kab.Bone, untuk mengetahui hasil belajar peserta didik sebelum dan sesudah diterapkan metode bermain peran (role playing) pada pembelajaran Al-Qur’an Hadits di kelas IV MI Al-Karimah Tanrung Kec.Ajangale Kab.Bone, dan untuk mengetahi pengaruh penerapan metode bermain peran (role playing) pada pembelajaran Al-Qur’an Hadits terhadap hasil belajar peserta didik kelas IV MI Al-Karimah Tanrung Kec.Ajangale Kab.Bone. Sampel yang diambil dalam penelitian ini berjumlah 12 orang peserta didik dengan menggunakan teknik pengumpulan data yaitu tes, observasi dan dokumentasi. Analisis data menggunakan regresi sederhana dengan bantuan aplikasi SPSS 25.0. Berdasarkan hasil analisis deskriptif hasil belajar peserta didik kelas IV MI Al-Karimah Tanrung Kec. Ajangale Kab. Bone rata-rata nilai Post Tes setelah diolah kedalam SPSS 25.0 adalah 82,08 dan rata-rata nilai Pre tes adalah 64,17. Terdapat pengaruh metode bermain peran (role playing) pada pembelajaran Al-Qur’an Hadits terhadap hasil belajar peserta didik kelas IV MI Al-Karimah Tanrung Kec.Ajangale Kab.Bone. Hal ini dapat dilihat pada analisis uji t yang telah dilakukan dengan menggunakan Regresi sederhana dengan menggunakan SPSS menunjukkan Thit = 5,750 dengan Ttable = 2,262. Nilai signifikansi yang diperoleh tersebut lebih kecil dari (0,000 > 0,05). Dengan besaran pengaruh sebesar 76,8% dari hasil uji koefisien determinasi (R Square)

Anti-neuroinflammatory effects of Hibiscus sabdariffa Linn. (Roselle) on Lipopolysaccharides-induced Microglia and neuroblastoma cells

Hibiscus sabdariffa Linn. (roselle) is a polyphenol rich fruit. This study aimed to identify the neuroprotective effect of roselle on LPS-induced cell proliferation and nitric oxide-induced free radical in microglia and neuroblastoma cells. MTT assay was used to identify the appropriate concentration of roselle and LPS for microglia and neuroblastoma cells proliferation study. Griess assay were used to determine the level of nitric oxide accumulated based on the reaction of Griess to estimate the activity of iNOS in nitric oxide production. The results showed that roselle at the concentration of 50 μg/mL and 100 μg/mL and LPS at concentration of 1 μg/mL does not give cytotoxic effect towards microglia C8-B4 and neuroblastoma LN18 cells. The roselle treatment at 50 μg/mL and 100 μg/mL showed a protective effect on LPS-induced microglia C8-B4 cells. However, in neuroblastoma LN18 cells, no protective effect was seen on both 50 μg/mL and 100 μg/mL of roselle treatment following induction with 1 μg/mL of LPS. On the other hand, the production of nitric oxide (NO) was reduced when LPS-induced microglia C8-B4 cells were treated with 50 μg/mL of roselle. Treatment of roselle at concentration 100 μg/mL on LPS-induced neuroblastoma LN18 cells also reduced the production of nitric oxide. As a conclusion, roselle had the ability to give neuroprotective effect by the inhibition of LPS induction activity on microglia activation for normal and cancer cells at different concentrations

Neuroprotection of Tropical Fruit Juice Mixture via the Reduction of iNOS Expression and CRH Level in β-Amyloid-Induced Rats Model of Alzheimer’s Disease

This study aimed to determine the effects of tropical fruit juice mixture (pomegranate, white guava, and Roselle) on biochemical, behavioral, and histopathological changes of β-amyloid- (Aβ-) induced rats. Formulation 8 (F8) of tropical fruit juice mixture was chosen for this present study due to its high phenolic content and antioxidant capacity. Forty Wistar male rats were divided into five groups: dPBS (sham-operated control), dAβ (Aβ control), JPBS (F8 and PBS), JAβ (F8 and Aβ), and IBFAβ (ibuprofen and Aβ). F8 (5 ml/kg BW), and ibuprofen (10 ml/kg BW) was given orally daily for four weeks before the intracerebroventricular infusion of Aβ for two weeks. Histological analysis and neuronal count of hippocampus tissue in the Cornu Ammonis (CA1) region showed that supplementation with F8 was able to prevent Aβ-induced tissue damage and neuronal shrinkage. However, no significant difference in locomotor activity and novel object recognition (NOR) percentage was detected among different groups at day 7 and day 14 following Aβ infusion. Only effect of time differences (main effect of day) was observed at day 7 as compared to day 14, where reduction in locomotor activity and NOR percentage was observed in all groups, with F (1, 7) = 6.940, p<0.05 and F (1, 7) = 7.152, p<0.05, respectively. Besides, the MDA level of the JAβ group was significantly lower (p<0.01) than that of the dPBS group. However, no significant changes in SOD activity were detected among different groups. Significant reduction in plasma CRH level (p<0.05) and iNOS expression (p<0.01) in the brain was detected in the JAβ group as compared to the dAβ group. Hence, our current findings suggest that the tropical fruit juice mixture (F8) has the potential to protect the rats from Aβ-induced neurotoxicity in brain hippocampus tissue possibly via its antioxidant properties and the suppression of iNOS expression and CRH production