Increased expression of the interleukin-1 receptor-associated kinase (IRAK)-1 is associated with adipose tissue inflammatory state in obesity

Figure S2. Comparison of IRAK-1 gene and protein expression in the adipose tissue. The gene and protein expression of IRAK-1 in non-diabetic obese, overweight, and lean adipose tissue samples, 5 each, were determined by using real-time RT-PCR and immunohistochemistry, respectively, as described in Patients and Methods. The relative mRNA expression was measured as fold expression over average of control gene expression taken as 1. The protein expression was measured as intensity which was calculated by using Aperio positive pixel count algorithm software (version 9)