Vegetation indices based farm-level mustard crop classification for the analysis of cropping pattern in Rabi 2021 and change in crop trend 2019 to 2021 of Kota District, Rajasthan

Remote sensing technology is used to quickly investigate as an innovative, standardized, potentially cost-effective, and faster method for crop acreage estimation. Furthermore, when compared to previous monitoring systems, Sentinel-2 satellite data has tremendous advantages since it delivers five-day interval, topographical, and up-to-date crop info at multiple phases. The main Rabi oil seed crop in Rajasthan is rapeseed and mustard. This study explores the use of the time series NDVI based farm level acreage estimation depending on the condition of the chlorophyll content. It also studies the changes in the cropping patterns and trends in Kota district, Rajasthan using the Google Earth Engine cloud platform along with the NCMS Mobile application for ground truth. Results indicate the reliability of the developed method for estimating acreage down to the farm level. Estimated Results for found to be in close agreement with authenticated government data. Two of the studied sub-districts showed significant cropping patterns. Classification accuracy for mustard ranged between 78-90 percent, while the overall classification accuracy 80-90 percent. The study concludes with the use of technology-based acreage estimations for faster and more reliable results

An advanced draft genome assembly of a desi type chickpea (Cicer arietinum L.)

Chickpea (Cicer arietinum L.) is an important pulse legume crop. We previously reported a draft genome assembly of the desi chickpea cultivar ICC 4958. Here we report an advanced version of the ICC 4958 genome assembly (version 2.0) generated using additional sequence data and an improved genetic map. This resulted in 2.7-fold increase in the length of the pseudomolecules and substantial reduction of sequence gaps. The genome assembly covered more than 94% of the estimated gene space and predicted the presence of 30,257 protein-coding genes including 2230 and 133 genes encoding potential transcription factors (TF) and resistance gene homologs, respectively. Gene expression analysis identified several TF and chickpea-specific genes with tissue-specific expression and displayed functional diversification of the paralogous genes. Pairwise comparison of pseudomolecules in the desi (ICC 4958) and the earlier reported kabuli (CDC Frontier) chickpea assemblies showed an extensive local collinearity with incongruity in the placement of large sequence blocks along the linkage groups, apparently due to use of different genetic maps. Single nucleotide polymorphism (SNP)-based mining of intra-specific polymorphism identified more than four thousand SNPs differentiating a desi group and a kabuli group of chickpea genotypes

First light preparations of the 4m ILMT

peer reviewedThe 4m International Liquid Mirror Telescope (ILMT) is a zenith-pointing optical observing facility at ARIES Devasthal observatory (Uttarakhand, India). The first light preparatory activities of the ILMT were accomplished in April 2022 followed by on-sky tests that were carried out at the beginning of May 2022. This telescope will perform a multi-band optical (SDSS $g'$, $r'$ and $i'$) imaging of a narrow strip (~$22'$) of sky utilizing the time-delayed integration technique. Single-scan ILMT images have an integration time of 102 sec and consecutive-night images can be co-added to further improve the signal-to-noise ratio. An image subtraction technique will also be applied to the nightly recorded observations in order to detect transients, objects exhibiting variations in flux or position. Presently, the facility is in the commissioning phase and regular operation will commence in October 2022, after the monsoon. This paper presents a discussion of the main preparation activities before first light, along with preliminary results obtained

Leishmania donovani: Immunostimulatory Cellular Responses of Membrane and Soluble Protein Fractions of Splenic Amastigotes in Cured Patient and Hamsters

Visceral leishmaniasis (VL), caused by the intracellular parasite Leishmania donovani, L. chagasi and L. infantum is characterized by defective cell-mediated immunity (CMI) and is usually fatal if not treated properly. An estimated 350 million people worldwide are at risk of acquiring infection with Leishmania parasites with approximately 500,000 cases of VL being reported each year. In the absence of an efficient and cost-effective antileishmanial drug, development of an appropriate long-lasting vaccine against VL is the need of the day. In VL, the development of a CMI, capable of mounting Th1-type of immune responses, play an important role as it correlate with recovery from and resistance to disease. Resolution of infection results in lifelong immunity against the disease which indicates towards the feasibility of a vaccine against the disease. Most of the vaccination studies in Leishmaniasis have been focused on promastigote- an infective stage of parasite with less exploration of pathogenic amastigote form, due to the cumbersome process of its purified isolation. In the present study, we have isolated and purified splenic amastigotes of L. donovani, following the traditional protocol with slight modification. These were fractionated into five membranous and soluble subfractions each i.e MAF1-5 and SAF1-5 and were subjected for evaluation of their ability to induce cellular responses. Out of five sub-fractions from each of membrane and soluble, only four viz. MAF2, MAF3, SAF2 and SAF3 were observed to stimulate remarkable lymphoproliferative, IFN-γ, IL-12 responses and Nitric Oxide production, in Leishmania-infected cured/exposed patients and hamsters. Results suggest the presence of Th-1 type immunostimulatory molecules in these sub-fractions which may further be exploited for developing a successful subunit vaccine from the less explored pathogenic stage against VL