Surface Plasmon Resonance biosensor analysis as a useful tool in FBDD

SPR (Surface Plasmon Resonance) biosensor instruments are more and more equipped to sensitively measure the binding characteristics of small molecules to their target. Via SPR biosensor measurements, not only the affinity of compounds but also other features such as the kinetics and thermodynamics aspects of binding can be determined. Furthermore, SPR is able to determine nonideal behavior of the fragment, such as aggregation and poor solubility binding. The use of SPR biosensors in various phases of the drug discovery process is evaluated. © 2010 Elsevier Ltd. All rights reserved

Synthesis and use of FSCPX, an irreversible adenosine A1 antagonist, as a 'receptor knock-down' tool

A new preparative synthetic route for the irreversible adenosine

Structure-based design, synthesis and structure-activity relationships of dibenzosuberyl- and benzoate-substituted tropines as ligands for acetylcholine-binding protein

Using structure-based optimization procedures on in silico hits, dibenzosuberyl- and benzoate substituted tropines were designed as ligands for acetylcholine-binding protein (AChBP). This protein is a homolog to the ligand binding domain of the nicotinic acetylcholine receptor (nAChR). Distinct SAR is observed between two AChBP species variants and between the α7 and α4β2 nAChR subtype. The AChBP species differences are indicative of a difference in accessibility of a ligand-inducible subpocket. Hereby, we have identified a region that can be scrutinized to achieve selectivity for nicotinic receptor subtypes. © 2011 Elsevier Ltd. All rights reserved