The Chemometric Approach for Verification of Paracetamol Level in Pharmacies Products Using Spectrophotometer UV-Vis

The objective of this study is to inspect paracetamol levels in Indonesian pharmacies and whether the level of paracetamol is matched the label given. The verification of paracetamol level using a chemometric approach such as partial least squares (PLS) and principal component regression (PCR) after measured with spectrophotometer UV. The outcome of this study showed the absorbance at 242 with an R2 (0.9991) and the linear range at 1.0 – 6.0 µg·mL-1. The accuracy was satisfactory and obtained at 99.18%. The satisfactory outcomes revealed the identification method has a good potential to be used in inspecting the paracetamol level in pharmaceutical products. Furthermore, the chemometric method used as a statistical measurement showed that there was no specific distinction to the validated approach. Thus, this approach can be applied in pharmacy industries specifically for small industries to ensure the medicine's safety before being distributed to the pharmacies

DETERMINATION OF VITAMIN C IN SPINACH (Amaranthus sp.) USING TITRATION METHOD

Vitamin C is a compound that is included in the group of water-soluble vitamins. The human body really needs vitamin C intake because it can act as an antioxidant which is closely related to collagen formation and iron absorption. Vitamin C is an organic compound that the human body needs in small amounts to maintain the body metabolic functions. Vitamin C is also one of the most important vitamins in increasing the body immunity This compound is very easy to find in fruits and vegetables, one of which is spinach. Spinach is a vegetable which generally contain vitamin C with an average of 158.54 mg/100 g. The research aims to analyze vitamin C levels in spinach using a titration method or also known as iodometric titration. This research method is included in an experimental study. The sample used in this research was obtained from Kotagede Market, Yogyakarta. The titration results for each spinach will be analyzed using Microsoft Excel, where the results have shown that the levels of vitamin C contained in snapper spinach (18.95 mg/100 g), picked spinach (46.39 mg/100 g), thorn spinach (14.52 mg /100 g) and red spinach (15.42 mg/100 g). Therefore, it can be concluded that this titration method can be used to accurately measure the vitamin C content in spinach. The iodometric titration method was chosen to analyze vitamin C levels because this application is very simple when compared to other methods. Ascorbic acid or vitamin C is known as a strong reducing agent and can simply be titrated using iodine solution

DETERMINATION OF ASCORBIC ACID CONCENTRATION IN MYRTACEAE USING THE IODOMETRIC TITRATION METHOD

Vitamins are essential compounds in food even though the content contained in these foods is small, and this is because vitamins have a vital role in the human body. Vitamin C or ascorbic acid is a secondary antioxidant compound that captures free radical compounds. Vitamin C content is easily found in vegetables and fruits. One of the fruits that contain vitamin C is guava. This study aims to determine and analyze vitamin C concentrations in several guava fruit types with the iodometric titration method. This type of research is a quantitative-qualitative experimental laboratory. The samples used in this study were guava in Myrtaceae family consisting of red guava (Psidium guajava L.), crystal guava (Psidium guajava L.), and red water guava (Syzygium aqueum). The study began with making 0.05 N iodine, iodine formation, then the 3% amylum indicators. The average sample content of red guava, crystal guava, and red water guava was 2.42 mg/100 grams. To conclude, the ascorbic acid concentration of red guava water is higher at 157 mg/100 g compared to the other two guavas, which are 80 mg/100 g and 87 mg/100 g