4,002 research outputs found
Serum levels of matrix metalloproteinases-2 and-9 and their tissue inhibitors in inflammatory neuromuscular disorders
We monitored serum levels of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) before and during intravenously applied immunoglobulin (IVIG) therapy in 33 patients with chronic immune-mediated neuropathies and myopathies and 15 controls. Baseline MMP-2 and TIMP-2 serum levels were lower and MMP-9 and TIMP-1 serum levels higher in all patients compared to age-matched controls. Eight days after IVIG treatment, MMP-2, TIMP-2, and TIMP-1 serum levels increased, while MMP-9 serum levels decreased, indicating tissue repair. After 60 days, MMP-9 levels increased, MMP-2 approached normal levels, while TIMP-1 and TIMP-2 serum levels were below day 8 levels, indicating relapsing tissue damage. Comparing the MMP/TIMP results with the clinical courses, IVIG treatment tended to change MMP/TIMP levels in a way that paralleled clinical improvement and relapse. In sum, during a distinct time period, IVIG therapy seems to be able to modulate VIMP-mediated tissue repair. Copyright (c) 2006 S. Karger AG, Basel
Antibiotic-specific differences in the response of Staphylococcus aureus to treatment with antimicrobiala combined with manuka honey
Skin infections caused by antibiotic resistant Staphylococcus aureus are a significant health problem worldwide; often associated with high treatment cost and mortality rate. Complex natural products like New Zealand (NZ) manuka honey have been revisited and studied extensively as an alternative to antibiotics due to their potent broad-spectrum antimicrobial activity, and the inability to isolate honey-resistant S. aureus. Previous studies showing synergistic effects between manuka-type honeys and antibiotics have been demonstrated against the growth of one methicillin-resistant S. aureus (MRSA) strain. We have previously demonstrated strong synergistic activity between NZ manuka-type honey and rifampicin against growth and biofilm formation of multiple S. arueus strains. Here, we have expanded our investigation using multiple S. aureus strains and four different antibiotics commonly used to treat S. aureus-related skin infections: rifampicin, oxacillin, gentamicin, and clindamycin. Using checkerboard microdilution and agar diffusion assays with S. aureus strains including clinical isolates and MRSA we demonstrate that manuka-type honey combined with these four antibiotics frequently produces a synergistic effect. In some cases when synergism was not observed, there was a significant enhancement in antibiotic susceptibility. Some strains that were highly resistant to an antibiotic when present alone become sensitive to clinically achievable concentrations when combined with honey. However, not all of the S. aureus strains tested responded in the same way to these combinational treatments. Our findings support the use of NZ manuka-type honeys in clinical treatment against S. aureus-related infections and extend their potential use as an antibiotic adjuvant in combinational therapy. Our data also suggest that manuka-type honeys may not work as antibiotic adjuvants for all strains of S. aureus, and this may help determine the mechanistic processes behind honey synergy
Two-neutron knockout from neutron-deficient Ar, S, and Si
Two-neutron knockout reactions from nuclei in the proximity of the proton
dripline have been studied using intermediate-energy beams of neutron-deficient
Ar, S, and Si. The inclusive cross sections, and also the
partial cross sections for the population of individual bound final states of
the Ar, S and Si knockout residues, have been determined
using the combination of particle and -ray spectroscopy. Similar to the
two-proton knockout mechanism on the neutron-rich side of the nuclear chart,
these two-neutron removal reactions from already neutron-deficient nuclei are
also shown to be consistent with a direct reaction mechanism.Comment: Phys. Rev. C, rapid communication, in pres
Two women presenting worsening cutaneous ulcers during pregnancy: diagnosis, immune response, and follow-up
Collapse of a Bose gas: kinetic approach
We have analytically explored temperature dependence of critical number of
particles for the collapse of a harmonically trapped attractively interacting
Bose gas below the condensation point by introducing a kinetic approach within
the Hartree-Fock approximation. The temperature dependence obtained by this
easy approach is consisted with that obtained from the scaling theory.Comment: Brief Report, 4 pages, 1 figure, Accepted in Pramana-Journal of
Physic
Spitzer Observations of Interstellar Object 1I/`Oumuamua
1I/`Oumuamua is the first confirmed interstellar body in our Solar System.
Here we report on observations of `Oumuamua made with the Spitzer Space
Telescope on 2017 November 21--22 (UT). We integrated for 30.2~hours at 4.5
micron (IRAC channel 2). We did not detect the object and place an upper limit
on the flux of 0.3 uJy (3sigma). This implies an effective spherical diameter
less than [98, 140, 440] meters and albedo greater than [0.2, 0.1, 0.01] under
the assumption of low, middle, or high thermal beaming parameter eta,
respectively. With an aspect ratio for `Oumuamua of 6:1, these results
correspond to dimensions of [240:40, 341:57, 1080:180] meters, respectively. We
place upper limits on the amount of dust, CO, and CO2 coming from this object
that are lower than previous results; we are unable to constrain the production
of other gas species. Both our size and outgassing limits are important because
`Oumuamua's trajectory shows non-gravitational accelerations that are sensitive
to size and mass and presumably caused by gas emission. We suggest that
`Oumuamua may have experienced low-level post-perihelion volatile emission that
produced a fresh, bright, icy mantle. This model is consistent with the
expected eta value and implied high albedo value for this solution, but, given
our strict limits on CO and CO2, requires another gas species --- probably H2O
--- to explain the observed non-gravitational acceleration. Our results extend
the mystery of `Oumuamua's origin and evolution
Consensus-Based Sorting of Neuronal Spike Waveforms
Optimizing spike-sorting algorithms is difficult because sorted clusters can rarely be
checked against independently obtained “ground truth” data. In most spike-sorting algorithms
in use today, the optimality of a clustering solution is assessed relative to some
assumption on the distribution of the spike shapes associated with a particular single unit
(e.g., Gaussianity) and by visual inspection of the clustering solution followed by manual
validation. When the spatiotemporal waveforms of spikes from different cells overlap, the
decision as to whether two spikes should be assigned to the same source can be quite subjective,
if it is not based on reliable quantitative measures. We propose a new approach,
whereby spike clusters are identified from the most consensual partition across an ensemble
of clustering solutions. Using the variability of the clustering solutions across successive
iterations of the same clustering algorithm (template matching based on K-means clusters),
we estimate the probability of spikes being clustered together and identify groups of spikes
that are not statistically distinguishable from one another. Thus, we identify spikes that are
most likely to be clustered together and therefore correspond to consistent spike clusters.
This method has the potential advantage that it does not rely on any model of the spike
shapes. It also provides estimates of the proportion of misclassified spikes for each of the
identified clusters. We tested our algorithm on several datasets for which there exists a
ground truth (simultaneous intracellular data), and show that it performs close to the optimum
reached by a support vector machine trained on the ground truth. We also show that
the estimated rate of misclassification matches the proportion of misclassified spikes measured
from the ground truth data
Can we continue research in splenectomized dogs? Mycoplasma haemocanis: Old problem - New insight
We report the appearance of a Mycoplasma haemocanis infection in laboratory dogs, which has been reported previously, yet, never before in Europe. Outbreak of the disease was triggered by a splenectomy intended to prepare the dogs for a hemorrhagic shock study. The clinical course of the dogs was dramatic including anorexia and hemolytic anemia. Treatment included allogeneic transfusion, prednisone, and oxytetracycline. Systematic follow-up (n=12, blood smears, antibody testing and specific polymerase chain reaction) gives clear evidence that persistent eradication of M. haemocanis is unlikely. We, therefore, had to abandon the intended shock study. In the absence of effective surveillance and screening for M. haemocanis, the question arises whether it is prudent to continue shock research in splenectomized dogs. Copyright (C) 2004 S. Karger AG, Basel
Information Literacy Needs Open Access or: Open Access is not Only for Researchers
The Open Access was initially (blandly) conceived in view not only of researchers but also of lay readers, then this perspective slowly faded out. The Information Literacy movement wants to teach citizens how to arrive at trustable information but the amount of paywalled knowledge is still big. So, their lines of development are somehow complementary: Information Literacy needs Open Access for the citizens to freely access high quality information while Open Access truly fulfils its scope when it is conceived and realized not only for the researchers (an aristocratic view which was the initial one) but for the whole society
Parameter selection for and implementation of a web-based decision-support tool to predict extubation outcome in premature infants
BACKGROUND: Approximately 30% of intubated preterm infants with respiratory distress syndrome (RDS) will fail attempted extubation, requiring reintubation and mechanical ventilation. Although ventilator technology and monitoring of premature infants have improved over time, optimal extubation remains challenging. Furthermore, extubation decisions for premature infants require complex informational processing, techniques implicitly learned through clinical practice. Computer-aided decision-support tools would benefit inexperienced clinicians, especially during peak neonatal intensive care unit (NICU) census. METHODS: A five-step procedure was developed to identify predictive variables. Clinical expert (CE) thought processes comprised one model. Variables from that model were used to develop two mathematical models for the decision-support tool: an artificial neural network (ANN) and a multivariate logistic regression model (MLR). The ranking of the variables in the three models was compared using the Wilcoxon Signed Rank Test. The best performing model was used in a web-based decision-support tool with a user interface implemented in Hypertext Markup Language (HTML) and the mathematical model employing the ANN. RESULTS: CEs identified 51 potentially predictive variables for extubation decisions for an infant on mechanical ventilation. Comparisons of the three models showed a significant difference between the ANN and the CE (p = 0.0006). Of the original 51 potentially predictive variables, the 13 most predictive variables were used to develop an ANN as a web-based decision-tool. The ANN processes user-provided data and returns the prediction 0–1 score and a novelty index. The user then selects the most appropriate threshold for categorizing the prediction as a success or failure. Furthermore, the novelty index, indicating the similarity of the test case to the training case, allows the user to assess the confidence level of the prediction with regard to how much the new data differ from the data originally used for the development of the prediction tool. CONCLUSION: State-of-the-art, machine-learning methods can be employed for the development of sophisticated tools to aid clinicians' decisions. We identified numerous variables considered relevant for extubation decisions for mechanically ventilated premature infants with RDS. We then developed a web-based decision-support tool for clinicians which can be made widely available and potentially improve patient care world wide
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