Determination of Total and Individual Anthocyanins in Raspberries Grown in South Serbia

Raspberry fruits of four cultivars (Willamette, Meeker, Polana and Malling Promise) grown in South Serbia were analyzed for total and individual anthocyanins. High-performance liquid chromatography with diode array detection (HPLC-DAD) was used to study individual anthocyanins. The major anthocyanin was cyanidin-3-sophoroside, followed by cyanidin-3-glucosylrutinoside, cyanidin-3-glucoside, pelargonidin-3-sophoroside and cyanidin-3-rutinoside. The monomeric anthocyanin contents of the acidified 80% methanol extracts were determined using the pH-differential method. The highest total and individual anthocyanins contain raspberries cv. Willamette, followed by cvs. Meeker, Polana and Malling Promise