Industry 4.0 Disruption and Its Neologisms in Major Industrial Sectors: A State of the Art

Very well into the dawn of the fourth industrial revolution (industry 4.0), humankind can hardly distinguish between what is artificial and what is natural (e.g., man-made virus and natural virus). Thus, the level of discombobulation among people, companies, or countries is indeed unprecedented. The fact that industry 4.0 is explosively disrupting or retrofitting each and every industrial sector makes industry 4.0 the famous buzzword amongst researchers today. However, the insight of industry 4.0 disruption into the industrial sectors remains ill-defined in both academic and nonacademic literature. The present study aimed at identifying industry 4.0 neologisms, understanding the industry 4.0 disruption and illustrating the disruptive technology convergence in the major industrial sectors. A total of 99 neologisms of industry 4.0 were identified. Industry 4.0 disruption in the education industry (education 4.0), energy industry (energy 4.0), agriculture industry (agriculture 4.0), healthcare industry (healthcare 4.0), and logistics industry (logistics 4.0) was described. The convergence of 12 disruptive technologies including 3D printing, artificial intelligence, augmented reality, big data, blockchain, cloud computing, drones, Internet of Things, nanotechnology, robotics, simulation, and synthetic biology in agriculture, healthcare, and logistics industries was illustrated. The study divulged the need for extensive research to expand the application areas of the disruptive technologies in the industrial sectors

The metastatic T-cell hybridoma antigen/P-selectin glycoprotein ligand 1 is required for hematogenous metastasis of lymphomas

Using variants of the murine BW5147 lymphoma cell-line, we have previously identified 3 monoclonal antibodies (MAbs) that discriminate between metastatic and nonmetastatic BW5147-derived T-cell hybridomas and lymphomas, as well as BW5147-unrelated T-lymphomas. These MAbs were reported to recognize an identical membrane-associated sialoglycoprotein, termed "metastatic T-cell hybridoma antigen" (MTH-Ag). Here, we document that the expression pattern of the MTH-Ag on metastatic and nonmetastatic BW5147 variants correlates with that of the P-selectin glycoprotein ligand 1 (PSGL-1), a sialomucin involved in leukocyte recruitment to sites of inflammation. Moreover, the MAbs against the MTH-Ag recognize PSGL-1 when it is transfected in MTH-Ag-negative BW5147 variants, suggesting that the MTH-Ag is PSGL-1. Overexpression of MTH-Ag/PSGL-1 in MTH-Ag-negative BW5147 variants did not affect their in vivo malignancy. Yet, down-regulation of MTH-Ag/PSGL-1 expression on metastatic, MTH-Ag-positive BW5147 variants, using an RNA interference (RNAi) approach, resulted, in a dose-dependent manner, in a significant reduction of liver and spleen colonization and a delay in mortality of the recipient mice upon intravenous inoculation. Collectively, these results demonstrate that, although MTH-Ag/PSGL-1 overexpression alone may not be sufficient for successful dissemination and organ colonization, MTH-Ag/PSGL-1 plays a critical role in hematogenous metastasis of lymphoid cancer cells

Development of Novel Efficient SIN Vectors with Improved Safety Features for Wiskott–Aldrich Syndrome Stem Cell Based Gene Therapy

Gene therapy is a promising therapeutic approach to treat primary immunodeficiencies. Indeed, the clinical trial for the Wiskott–Aldrich Syndrome (WAS) that is currently ongoing at the Hannover Medical School (Germany) has recently reported the correction of all affected cell lineages of the hematopoietic system in the first treated patients. However, an extensive study of the clonal inventory of those patients reveals that LMO2, CCND2 and MDS1/EVI1 were preferentially prevalent. Moreover, a first leukemia case was observed in this study, thus reinforcing the need of developing safer vectors for gene transfer into HSC in general. Here we present a novel self-inactivating (SIN) vector for the gene therapy of WAS that combines improved safety features. We used the elongation factor 1 alpha (EFS) promoter, which has been extensively evaluated in terms of safety profile, to drive a codon-optimized human WASP cDNA. To test vector performance in a more clinically relevant setting, we transduced murine HSPC as well as human CD34+ cells and also analyzed vector efficacy in their differentiated myeloid progeny. Our results show that our novel vector generates comparable WAS protein levels and is as effective as the clinically used LTR-driven vector. Therefore, the described SIN vectors appear to be good candidates for potential use in a safer new gene therapy protocol for WAS, with decreased risk of insertional mutagenesis.Fil: Avedillo Diez, Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina. Hannover Medical School; AlemaniaFil: Zychlinski, Daniela. Hannover Medical School; AlemaniaFil: Coci, Emanuele G.. Hannover Medical School; AlemaniaFil: Galla, Melanie. Hannover Medical School; AlemaniaFil: Modlich, Ute. Hannover Medical School; AlemaniaFil: Dewey, Ricardo. Hannover Medical School; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; ArgentinaFil: Schwarzer, Adrian. Hannover Medical School; AlemaniaFil: Maetzig, Tobias. Hannover Medical School; AlemaniaFil: Mpofu, Nonsikelelo. Hannover Medical School; AlemaniaFil: Jaeckel, Elmar. Hannover Medical School; AlemaniaFil: Boztug, Kaan. Hannover Medical School; AlemaniaFil: Baum, Christopher. Hannover Medical School; AlemaniaFil: Klein, Christoph. Hannover Medical School; AlemaniaFil: Schambach, Axel. Hannover Medical School; Alemani