Temperature dependence of planktonic metabolism in the subtropical North Atlantic Ocean

The temperature dependence of planktonic metabolism in the subtropical North Atlantic Ocean was assessed on the basis of measurements of gross primary production (GPP), community respiration (CR) and net community production (NCP), as well as experimental assessments of the response of CR to temperature manipulations. Metabolic rates were measured at 68 stations along three consecutive longitudinal transects completed during the Malaspina 2010 Expedition, in three different seasons. Temperature gradients were observed in depth and at basin and seasonal scale. The results showed seasonal variability in the metabolic rates, the highest rates being observed during the spring transect. The overall mean integrated GPP / CR ratio was 1.39 ± 0.27 decreasing from winter to summer, and the NCP for the subtropical North Atlantic Ocean during the cruises exhibits net autotrophy (NCP > 0) in about two-thirds (66%) of the total sampled communities. Also, we reported the activation energies describing the temperature dependence of planktonic community metabolism, which was generally higher for CR than for GPP in the subtropical North Atlantic Ocean, as the metabolic theory of ecology predicts. Furthermore, we made a comparison of activation energies describing the responses to in situ temperature in the field (EaCR = 1.64 ± 0.36 eV) and those derived experimentally by temperature manipulations (EaCR = 1.45 ± 0.6 eV), which showed great consistency

Short-term variability of primary production and inorganic nitrogen uptake related to the environmental conditions in a shallow coastal area (Gulf of Trieste, N Adriatic Sea)

Primary production (PP) and, nitrate (QNO(3)) and ammonium (QNH(4)) uptakes were measured together with other environmental parameters from October 1999 to February 2001 in the Gulf of Trieste (N Adriatic Sea). Their trends showed a high variability because of the combined effects of meteorological conditions, water circulation and river discharges. PP ranged from 0.2 to 1.5.9 mumol C dm(-3) d(-1), whereas QNO(3) varied from 0.8 to 442 nmol N dm(-3) d(-1), showing a trend similar to that of carbon basically ascribable to the autotrophic activity. QNH(4) ranged from 20 to 1308 nmol N dm-3 d-' and it reached the highest values during the declining phases of phytoplankton blooms, indicating that bacterial community can also be involved in its uptake. Regenerated PP generally prevailed over the new production (depth-integrated f-ratios from 0.05 to 0.50). C/N uptake ratios by planktonic community (annual average of 16 +/- 11) showed the repetitive carbon overconsumption (23-33) during periods of high production, and lower values (2-13) during: the post-bloom phases and in the months of scarce autotrophic activity. Residence time of freshwater in the area (1 d in November and January, up to 23 d in July) indicated the fast export of low salinity waters in winter and their longer permanence in summer. These values were closer to the ammonium turnover times (1-34 d) than to the nitrate ones (2-831 d). Riverine nitrate load (3-67 t N d-') generally exceed the biological demand of this nutrient (uptakes from 0.2 to 8 t N d-'), whereas the ammonium load (0.1-33 t N d(-1)) was almost always insufficient (uptakes, from 2.6 to 33 t N d(-1)). These results evidenced the major role of physical transport and recycling processes to regulate, respectively, nitrate and ammonium availability in this shallow ecosystem.La production primaire et l’assimilation de nitrates et d’ammonium ont été mesurées en même temps que les facteurs du milieu entre octobre 1999 et février 2001 dans le golfe de Trieste. La variabilité est élevée en raison de l’action combinée des conditions météorologiques, de la circulation et des apports des rivières. La production primaire varie entre 0,2 et 15,9 μmol C dm–3 j–1 alors que l’assimilation de nitrates va de 0,8 à 442 nmol N dm–3 j–1, montrant une tendance identique au carbone lié à la production autotrophe. L’assimilation d’ammonium varie entre 20 et 1308 nmol N dm–3 j–1 et elle atteint ses valeurs maximales durant le déclin de la floraison planctonique, indiquant que la communauté bactérienne est impliquée dans cette assimilation. La production primaire régénérée surpasse la production nouvelle (le facteur f intégré en fonction de la profondeur varie entre 0,05 et 0,5). Les taux C/N d’assimilation de la communauté planctonique (moyenne annuelle de 16 ± 11) soulignent la surconsommation répétitive de carbone (23–33) durant les phases de production actives ; les valeurs minimales (2–13) caractérisent les phases postérieures à la floraison et les mois de faible activité autotrophe. Le temps de résidence d’eau douce dans la zone (d’un jour en novembre et janvier à 23 jours en juillet) indiquent l’exportation rapide d’eau de basse salinité en hiver et leur permanence en été. Ces valeurs sont plus proches des temps de renouvellement de l’ammonium (1 à 34 jours) que des nitrates (2 à 831 jours). L’apport de nitrates par les fleuves (3–67 t N j–1) surpasse les besoins (0,2 à 8 t N j–) alors que l’apport d’ammonium (0,1–3,3 t N j–1) est presque toujours insuffisant (assimilation de 2,6 à 33 t N j–). Ces résultats mettent en lumière le rôle majeur du transport et du recyclage dans la régulation de la disponibilité en nitrate et ammonium dans des écosystèmes de faible profondeur

Microbial mechanisms coupling carbon and phosphorus cycles in phosphorus-limited northern Adriatic Sea

The coastal northern Adriatic Sea receives pulsed inputs of riverine nutrients, causing phytoplankton blooms and seasonally sustained dissolved organic carbon (DOC) accumulation-hypothesized to cause episodes of massive mucilage. The underlying mechanisms regulating P and C cycles and their coupling are unclear. Extensive biogeochemical parameters, processes and community composition were measured in a 64-day mesocosms deployed off Piran, Slovenia. We followed the temporal trends of C and P fluxes in P-enriched (P+) and unenriched (P-) mesocosms. An intense diatom bloom developed then crashed; however, substantial primary production was maintained throughout, supported by tightly coupled P regeneration by bacteria and phytoplankton. Results provide novel insights on post-bloom C and P dynamics and mechanisms. 1) Post-bloom DOC accumulation to 186\u3bcM remained elevated despite high bacterial carbon demand. Presumably, a large part of DOC accumulated due to the bacterial ectohydrolytic processing of primary productivity that adventitiously generated slow-to-degrade DOC; 2) bacteria heavily colonized post-bloom diatom aggregates, rendering them microscale hotspots of P regeneration due to locally intense bacterial ectohydrolase activities; 3) Pi turnover was rapid thus suggesting high P flux through the DOP pool (dissolved organic phosphorus) turnover; 4) Alpha- and Gamma-proteobacteria dominated the bacterial communities despite great differences of C and P pools and fluxes in both mesocosms. However, minor taxa showed dramatic changes in community compositions. Major OTUs were presumably generalists adapted to diverse productivity regimes.We suggest that variation in bacterial ectohydrolase activities on aggregates, regulating the rates of POM\u2192DOM transition as well as dissolved polymer hydrolysis, could become a bottleneck in P regeneration. This could be another regulatory step, in addition to APase, in the microbial regulation of P cycle and the coupling between C and P cycles. \ua9 2013