Diffuse reflectance imaging for non-melanoma skin cancer detection using laser feedback interferometry

We propose a compact, self-aligned, low-cost, and versatile infrared diffuse-reflectance laser imaging system using a laser feedback interferometry technique with possible applications in in vivo biological tissue imaging and skin cancer detection. We examine the proposed technique experimentally using a three-layer agar skin phantom. A cylindrical region with a scattering rate lower than that of the surrounding normal tissue was used as a model for a non-melanoma skin tumour. The same structure was implemented in a Monte Carlo computational model. The experimental results agree well with the Monte Carlo simulations validating the theoretical basis of the technique. Results prove the applicability of the proposed technique for biological tissue imaging, with the capability of depth sectioning and a penetration depth of well over 1.2 mm into the skin phantom

Three-dimensional imaging of cell and extracellular matrix elasticity using quantitative micro-elastography

Funding: Australian Research Council; Cancer Council Western Australia; Industrial Transformation Training Centre; The William and Marlene Schrader Trust of the University of Western Australia.Recent studies in mechanobiology have revealed the importance of cellular and extracellular mechanical properties in regulating cellular function in normal and disease states. Although it is established that cells should be investigated in a three-dimensional (3-D) environment, most techniques available to study mechanical properties on the microscopic scale are unable to do so. In this study, for the first time, we present volumetric images of cellular and extracellular elasticity in 3-D biomaterials using quantitative micro-elastography (QME). We achieve this by developing a novel strain estimation algorithm based on 3-D linear regression to improve QME system resolution. We show that QME can reveal elevated elasticity surrounding human adipose-derived stem cells (ASCs) embedded in soft hydrogels. We observe, for the first time in 3-D, further elevation of extracellular elasticity around ASCs with overexpressed TAZ; a mechanosensitive transcription factor which regulates cell volume. Our results demonstrate that QME has the potential to study the effects of extracellular mechanical properties on cellular functions in a 3-D micro-environment.Publisher PDFPeer reviewe

Multimodal mechano-microscopy reveals mechanical phenotypes of breast cancer spheroids in three dimensions

Funding: This study was supported by the Australian Research Council, The Ian Potter Foundation, Department of Health, Western Australia (WANMA2021/1), Research Training Program Scholarship, Hackett Postgraduate Research Scholarship, and Cancer Council Western Australia (RP G0039). P.W. was supported by the 1851 Research Fellowship from the Royal Commission. B.F.K acknowledges funding from the NAWA Chair programme of the Polish National Agency for Academic Exchange and from the National Science Centre, Poland.Cancer cell invasion relies on an equilibrium between cell deformability and the biophysical constraints imposed by the extracellular matrix (ECM). However, there is little consensus on the nature of the local biomechanical alterations in cancer cell dissemination in the context of three-dimensional (3D) tumor microenvironments (TMEs). While the shortcomings of two-dimensional (2D) models in replicating in situ cell behavior are well known, 3D TME models remain underutilized because contemporary mechanical quantification tools are limited to surface measurements. Here, we overcome this major challenge by quantifying local mechanics of cancer cell spheroids in 3D TMEs. We achieve this using multimodal mechano-microscopy, integrating optical coherence microscopy-based elasticity imaging with confocal fluorescence microscopy. We observe that non-metastatic cancer spheroids show no invasion while showing increased peripheral cell elasticity in both stiff and soft environments. Metastatic cancer spheroids, however, show ECM-mediated softening in a stiff microenvironment and, in a soft environment, initiate cell invasion with peripheral softening associated with early metastatic dissemination. This exemplar of live-cell 3D mechanotyping supports that invasion increases cell deformability in a 3D context, illustrating the power of multimodal mechano-microscopy for quantitative mechanobiology in situ.Peer reviewe

Dual-modality confocal laser feedback tomography for highly scattering medium

Combination of optical modalities is a common practice to improve the efficiency of biomedical optics systems. We propose confocal laser feedback tomography for volumetric dualmodality imaging. Two major modalities of laser Doppler flowmetry and reflectance confocal microscopy were integrated and the signal were concurrently acquired for cross-sectional and volumetric imaging, by means of a compact laser scanning system. The technique was applied to a two-layered skin tissue phantom containing a 200 µm diameter Intralipid flow channel that features functional and morphological changes at the same time. Results show the potential for concurrent three-dimensional mapping of dynamic and static inhomogeneities in a highly scattering medium which can improve contrast in non-invasive biological tissue imaging, providing a confocal image in addition to the Doppler flowmetry image

Experimental study and analysis of CO2 and SO2 absorption in various water-based nanofluids by response surface methodology

Abstract The absorption of acidic gases in the oil and gas industries is important due to their toxicity and corrosive effects. Recently, the application of nanofluids based on aqueous or organic solvents as absorbents has been examined by a variety of researchers. In this study, a single bubble column was exploited to study the effect of water-based nanofluids on the absorption processes of SO2 and CO2 using response surface methodology (RSM) based on Box-Behnken three-level experiment design. With this in mind, CO2 and SO2 are separately injected at the bottom of a bubble column filled with one of the nanofluids: Al2O3-water, SiO2-water, or ZnO-water for each experiment. Then, the rate of SO2 or CO2 absorption in the nanofluids has been elucidated. The effect of important parameters including the weight fraction of the nanoparticles (NPs) (0.01, 0.055, and 0.1 wt.%), gas–liquid contact time (150, 300, and 450 s), and the diameter of nozzle for gas injection (0.46, 0.57, and 0.68 mm) have been studied. Results revealed that the maximum molar flux of both gases was observed in the ZnO-water nanofluid, followed by the SiO2-water nanofluid. In addition, increasing the nanoparticle mass fraction and the bubble size causes the molar flux to rise. However, increasing the gas–liquid contact time causes the molar flux of the mentioned gases to decrease. Finally, a set of the accurate equations has been proposed to predict the molar flux of SO2 and CO2 in the various nanofluids assessed in this work

Effect of the optical numerical aperture on the Doppler spectrum in laser Doppler velocimetry

We have investigated the effect of the optical system design on the characteristics of the Doppler spectrum of a laser Doppler velocimeter, by means of a Monte Carlo simulation model. We show that numerical aperture is one of the parameters that strongly affect the full width at half maximum and signal to noise ratio of the Doppler spectrum. The profile and intensity of the enhanced backscattering of the laser beam from a diffusive rough aluminum disk surface is measured using a range of lenses with different focal lengths and the results are incorporated into the Monte Carlo model

Polarization-sensitive laser feedback interferometry for specular reflection removal

Specular reflection from the surface of targets or prepared specimens represents a significant problem in optical microscopy and related optical imaging techniques as usually the surface reflection does not contribute to the desired signal. Solutions exist for many of these imaging techniques; however, remedial techniques for imaging based on laser feedback interferometry (LFI) are absent. We propose a reflection cancellation technique based on crossed-polarization filtering that is tailored for a typical LFI configuration. The technique is validated with three experimental designs, and a significant improvement of about 40 dB in the ratio of the diffuse and specular LFI signal is observed. Applications of this principle extend from specular reflection removal to characterization of target materials in industrial to biomedical domains

Effect of the optical system on the Doppler spectrum in laser-feedback interferometry

We present a comprehensive analysis of factors influencing the morphology of the Doppler spectrum obtained from a laser-feedback interferometer. We explore the effect of optical system parameters on three spectral characteristics: central Doppler frequency, broadening, and signal-to-noise ratio. We perform four sets of experiments and replicate the results using a Monte Carlo simulation calibrated to the backscattering profile of the target. We classify the optical system parameters as having a strong or weak influence on the Doppler spectrum. The calibrated Monte Carlo approach accurately reproduces experimental results, and allows one to investigate the detailed contribution of system parameters to the Doppler spectrum, which are difficult to isolate in experiment. (C) 2014 Optical Society of Americ