2 research outputs found
Correlation between major depressive disorder and circulating natural killer cells
Background: Major depressive disorder is a mental disorder characterized by a pervasive and persistent low mood that is accompanied by low self-esteem and loss of interest or pleasure in normally enjoyable activities. Depression is associated with multiple immunological disorders. Aim of the present study was to determine correlation between percentage of circulating NK cells and major depressive disorder.Materials and Methods: Patients older than 18 years with the desire to participate were enrolled in this study. For depression evaluation, we used the Hamilton Depression Rating Scale and for determination of percentage of NK cells in peripheral blood, flow cytometry method was used. Results: Our results showed that in patients with major depressive disorder, numbers of circulating NK cells have significantly reduced. Conclusion: According to our findings, depression is associated with “immune suppression”. NK cells are important in early phase of immunological surveillance versus viral infections and tumors. Indeed, depressive patients are susceptible to cancers and infections
Terminal Deoxynucleotidyl Transferase (TdT) Inhibiti on of Cord Blood Derived B and T Cells Expansion
Purpose: Terminal deoxynucleotidyl transferase(TdT) is a DNA polymerase that is present in immature pre-B and pre-T cells.
TdT inserts N-nucleotides to the V (D) J gene segment during rearrangements of
genes, therefore, it plays a vital role in the development and variation of the immune
system in vertebrates. Here we evaluated the
relationship between cytokines like interleukin-2 (IL-2),
interleukin-7 (IL-7), and interleukin-15 (IL-15) and
TdT expression in cord blood mononuclear cells and also effect of inhibition in
the expansion of B and T cells derived from cord blood.
Methodes: The cord blood
mononuclear cells were cultured with different combination of cytokines for
21days, which they were harvested in definite days (7, 14 and 21) and evaluated
by flow cytometry.
Results: Our data
indicated that TdT expression increased in cord
blood mononuclear cells using immune cell key cytokines without being dependent
on the type of cytokines. TdT inhibition reduced both the expansion of B and T
cells derived from cord blood and also declined the apoptosis and proliferation.
Considered together, TdT played an important role in the control of the
expansion of B and T cells derived from cord blood.
Conclusion: considered together, it was observed that TdT
expression was increased by cytokines and TdT inhibition not only reduced B and
Tcells derived from cord blood, but it also affected the rate of apoptosis and
proliferation