Evaluation of the Effect of Ascorbic Acid and Sucrose Foliar Application on some Quantitative and Qualitative Characteristics of Cucurbita pepo var. Styriaca

Introduction Pumpkin (Cucurbita pepo) is a medicinal plant belonging to the Cucurbitaceae family and the order Cucurbitals. The seeds of this plant are a rich source of essential oils and proteins for the body. It is used in the production of various drugs such as Peponen, Pepostrin, Grunfig and treatment of prostate swelling, urinary tract inflammation, atherosclerosis, gastrointestinal regulation, etc. Since the components of medicinal plants are low at natural condition, and could be increased by means of different environmental conditions, nutrition or application of elicitors; thus, it is necessary to work on mentioned parameters effects on quantitative and qualitative attributes of medicinal plants. Recent years, many researches have been done based on natural components for increment of yield and secondary metabolites of medicinal plants. Ascorbic acid is one of these materials which its effect on plant growth has been validated. In the management of agricultural inputs, especially in the cultivation of medicinal plants, the application of substances that have the least harmful side effects on human health and the environment is recommended. Meanwhile, sucrose and ascorbic acid are healthy substances to improve growth and increase crop yield. Therefore, the aim of the present study was to determine the effect of these two substances on yield, yield components and phytochemical characteristics of pumpkins. Materials and Methods  Pumpkin seeds were prepared from Pakan Bazr Esfahan by purity of 99%. Then, planted in a farm of 500 m2 at Behshar. After plant growth, spray treatments were conducted at three times as before flowering, onset of flowering and fruit set stages. This experiment was conducted in factorial with sucrose factor at four levels (0, 5, 10, 15 g.l-1) and ascorbic acid factor at four levels (0, 15, 30, 45 mM), based on a randomized complete block design with three replications. The studied characteristics included number of leaves and fruits, plant yield, 1000-seed weight, total number of seeds, number of healthy seeds, percentage of healthy seeds, number of blank (deaf) seeds, percentage of blank seeds, total chlorophyll, antioxidant activity, phenol, flavonoids, protein and oil percentage. Statistical analysis of data was performed using SAS statistical software and comparison of mean was performed using the least significant difference (LSD) at the level of 5% probability. Figures were graphed with Excel software. Results and Discussion  According to this study results, the effect of foliar application of sucrose and ascorbic acid and their interaction on most of the studied traits was significant. Application of 15 g.l-1 sucrose with 15 mM ascorbic acid increased the number of fruits to 1.68 per plant, which showed an increase compared to the control treatment. The highest total number of seeds with an average of 464 seeds per fruit was obtained by applying 5 g.l-1 sucrose with 45 mM ascorbic acid, which compared to the control (247.33) recorded an increase of 87.60%. The highest total chlorophyll content was measured with an average of 2.081 (mg.g-1 fresh weight) using 5 g.l-1 sucrose with 15 mM ascorbic acid, which showed an increase of 1.81% compared to the control treatment (2.044). Also, application of 15 g.l-1 sucrose along with 15 mM ascorbic acid increased protein by 40.03%, which showed an increase of 79.26% compared to the control (22.33). Other results indicate that increasing the amount of seed oil up to 44.50% is available with the application of 15 g.l-1 sucrose with 30 mM ascorbic acid and also with the application of 10 g.l-1 sucrose with 45 mM ascorbic acid; which had an increase of 16.61% compared to the control (38.16). The results of the present study showed that the application of combined ratios of sucrose and ascorbic acid has been effective in improving the quantitative and qualitative attributes of pumpkin, including protein content and percentage of pumpkin seed oil. Conclusion  Since the treatment of sucrose 10 g.l-1 with 45 mM ascorbic acid significantly affected most of important attributes such as total antioxidant activity, total flavonoids, protein content and high oil content, therefore, this combination of treatment can be applied to increase the quality of pumpkin seeds. However, if only quantity is important, the treatment of sucrose 15 g.l-1 with 15 mM ascorbic acid, which caused the highest number of fruits per plant, the highest yield as well as the highest protein, can be recommended

Effects of silibinin on hair follicle stem cells differentiation to neural-like cells

The hair follicle stem cells are an abundant and easily accessible source of pluripotent adult stem cells. Hairfollicle stem cells differentiate into neurons and glial cells in vitro and express stem cell markers such as tubulin, RIP, P75, and S100. Silibinin, a flavonolignan and the active component of Milk thistle (Silybum Marianum) has been used as a dietary supplement and herbal medication. The aim of the present study was to investigate the effects of Silibinin on hair follicle stem cells differentiation to neural-like cells. The bulge region of the rat whisker was isolated and cultured in Dulbecco's modified Eagle's medium supplemented with different concentrations of silibinin and Neurotrophin-3. One week later, bulge cells immunostained with Nestin and p75 and then with tubulin. The morphological and biological features of cultured bulge cells were observed by light microscopy. Present data demonstrated that silibinin (0.5 μg mL-1) can promote differentiation of hair follicle stem cells to neural-like cells. © 2011 Academic Journals Inc

Elevation of cyclic AMP causes an imbalance between NF-κB and p53 in NALM-6 cells treated by doxorubicin

We previously showed that cAMP can inhibit DNA damage-induced wild type p53 accumulation in human pre-B NALM-6 cells, leading to a profound reduction of their apoptotic response. Here, we provide evidence for the potentiation of DNA damage-induced NF-κB activation by cAMP. We found that inhibition of NF-κB activation prevents the inhibitory effect of cAMP on doxorubicin-induced apoptosis. Moreover, cAMP exerts its inhibitory effect on doxorubicin-induced apoptosis in a PKA-independent manner. The present study also shows that elevation of cAMP prolongs the phosphorylation of IκB and subsequent activation of NF-κB in doxorubicin treated NALM-6 cells in a proteasome-dependent manner. Taken together, our results demonstrate that cAMP abrogates the balance between apoptotic and antiapoptotic transcription factors that are hallmarks of DNA damage signaling. © 2010 Federation of European Biochemical Societies

The importance of CDC27 in cancer: molecular pathology and clinical aspects

Background: CDC27 is one of the core components of Anaphase Promoting complex/cyclosome. The main role of this protein is defined at cellular division to control cell cycle transitions. Here we review the molecular aspects that may affect CDC27 regulation from cell cycle and mitosis to cancer pathogenesis and prognosis. Main text: It has been suggested that CDC27 may play either like a tumor suppressor gene or oncogene in different neoplasms. Divergent variations in CDC27 DNA sequence and alterations in transcription of CDC27 have been detected in different solid tumors and hematological malignancies. Elevated CDC27 expression level may increase cell proliferation, invasiveness and metastasis in some malignancies. It has been proposed that CDC27 upregulation may increase stemness in cancer stem cells. On the other hand, downregulation of CDC27 may increase the cancer cell survival, decrease radiosensitivity and increase chemoresistancy. In addition, CDC27 downregulation may stimulate efferocytosis and improve tumor microenvironment. Conclusion: CDC27 dysregulation, either increased or decreased activity, may aggravate neoplasms. CDC27 may be suggested as a prognostic biomarker in different malignancies. © 2021, The Author(s)

The role of progesterone in cellular apoptosis of skin and lung in a bleomycin-injured mouse model

Systemic sclerosis is a female predominant, a fibrotic autoimmune disease in which disturbance in tissue homeostasis and cell turnover including cell apoptosis are central events in pathogenesis. Sex hormones are known as the important players in sexual dimorphism of autoimmune diseases and in tissue homeostasis. Progesterone influences autoimmune disease via its immunomodulatory effect or by its direct action on parenchymal cell function. On the other hand, this hormone impacts tissue homeostasis by acting on cell apoptosis in a different situation. The objective of this study was to examine the effect of progesterone on cellular apoptosis of skin and lung tissues in a mouse model of scleroderma. Four group of mice were involved in this study with 10 mice in each. The fibrotic model was induced by daily subcutaneous injection of bleomycin for 28 days. One week after initiation of fibrosis induction, mice received subcutaneous progesterone alone or with bleomycin for 21 days. Control group received only Phosphate buffered saline PBS. After 28 days, under lethal anesthesia skin and lung tissues were harvested for histological assessment and hydroxyproline measurement. Apoptosis in tissue sections was detected by TUNEL assay technique. Bleomycin administration induced fibrosis in skin and lung tissues. Severe apoptosis was seen in skin and lung tissues of the bleomycin-treated group (p0.05) or in the lung (p>0.05) did not alter apoptosis in bleomycin-treated animals. Our data confirm the role of apoptosis in the pathogenesis of fibrosis in this model; however, progesterone does not affect cellular apoptosis in skin and lung tissues of bleomycin-injured animals. Copyright© February 2019, Iran J Allergy Asthma Immunol. All rights reserved

Genetic Variants of Cytochrome b-245, Alpha Polypeptide Gene and Premature Acute Myocardial Infarction Risk in An Iranian Population

Background: Oxidative stress induced by superoxide anion plays critical roles in the pathogenesis of coronary artery disease (CAD) and hence acute myocardial infarction (AMI). The major source of superoxide production in vascular smooth muscle and endothelial cells is the NADPH oxidase complex. An essential component of this complex is p22phox, that is encoded by the cytochrome b-245, alpha polypeptide (CYBA) gene. The aim of this study was to investigate the association of CYBA variants (rs1049255 and rs4673) and premature acute myocardial infarction risk in an Iranian population. Methods: The study population consisted of 158 patients under the age of 50 years, with a diagnosis of premature AMI, and 168 age-matched controls with normal coronary angiograms. Genotyping of the polymorphisms was performed by the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). Results: There was no association between the genotypes and allele frequencies of rs4673 polymorphism and premature acute myocardial infarction (P>0.05). A significant statistical association was observed between the genotypes distribution of rs1049255 polymorphism and AMI risk (P=0.037). Furthermore, the distribution of AA+AG/GG genotypes was found to be statistically significant between the two groups (P=0.011). Conclusions: Our findings indicated that rs1049255 but not rs4673 polymorphism is associated with premature AMI

Gelatinases Increase in Bleomycin-induced Systemic Sclerosis Mouse Model

Systemic sclerosis is a fibrotic autoimmune disease in which aberrant remodeling of the extracellular matrix in organs disturbs their functionalities. The aim of this study was to investigate the expression of gelatinases on systemic sclerosis. Consequently, a mouse model of systemic sclerosis was employed and the gelatinolytic activity of gelatinases was evaluated on the fibrotic tissues of this model. Two groups of ten mice were considered in this work: a group of systemic sclerosis model and control group. For the generation of systemic sclerosis model, mice received bleomycin, while the control group was subjected to phosphate buffered saline (PBS) reception. Mice were tested for fibrosis by using trichrome staining, hydroxyproline measurement and α-SMA detection in tissue sections. Additionally, the gelatinolytic activity of matrix metalloproteinase 2 and matrix metalloproteinase 9 were measured using gelatin zymography in lungs and skin tissue homogenates. The obtained results indicated that subcutaneous injection of bleomycin-induced fibrosis in skin and lung tissues of mice. Pro and active forms of matrix methaloproteinase 9 were increased in fibrotic lung tissues (p<0.05 and p<0.01, respectively), while, the gelatinolytic activity of MMP2 was unaffected in these tissues. Additionally, in skin tissues of bleomycin-treated animals, both pro and active forms of MMP9 and MMP2 were increased (p<0.05). Pro and active forms of gelatinases increase differently in skin and lung tissues of bleomycin-induced scleroderma

Human leukocyte antigen class I (A, B) and class II (DRB1) allele and haplotype frequencies in Iranian patients with Buerger's disease

Objective: The aim of this study was to investigate the human leukocyte antigen (HLA) class I (HLA-A and HLA-B) and II (HLA-DRB1) allele and haplotype frequencies in a group of Iranian patients with Buerger's disease (BD) in comparison with a normal healthy control group. Methods: A total of 70 unrelated male patients and 100 healthy controls from Sina Hospital, Tehran, Iran, belonging to the same ethnic background, were enrolled in this case-control study. HLA-A, B, and DRB1 typing were performed by polymerase chain reaction with sequence-specific primers (PCR-SSP). Results: The results of this case-control study showed that the frequency of the HLA-A*03:01 (odds ratio (OR) = 2.88, P value (Pv) =.002), HLA-A*29:01 (OR = 15.31, Pv <.001), HLA-DRB1*04:02 (OR = 3.41, Pv <.001), and HLA-DRB1*16:01 (OR = 8.16, Pv <.001) was significantly higher in BD patients compared with healthy controls, whereas the frequency of the HLA-DRB1*01:01 (OR = 0.03, Pv <.001) was significantly lower in BD patients. The most frequent extended haplotypes in our patients were HLA-A*02:01-B*55:01-DRB1*04:03. Conclusion: This study is the first study evaluating an association between the HLA pattern and BD in the patients with BD from North West and North Iran. © 2020 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Lt

Fluoxetin upregulates connexin 43 expression in astrocyte

Introduction: Recent studies have shown that astrocytes play major roles in normal and disease condition of the central nervous system including multiple sclerosis (MS). Molecular target therapy studies in MS have revealed that connexin-43 (Cx43) and Aquaporin-4 (AQP4) contents of astrocytes undergo expression alteration. Fluoxetine had some effects in MS patients unrelated to its known antidepressant effects. Some of fluoxetine effects were attributed to its capability of cAMP signaling pathway stimulation. This study aimed to investigate possible acute effects of fluoxetine on Cx43 and AQP4 expression in astrocyte. Methods: Astrocytoma cells were treated for 24 hours with fluoxetine (10 and 20 μg/ml) with or without adenyl cyclase (AC) and protein kinase A (PKA) inhibition. Cx43 expression at both mRNA and protein levels and AQP4 expression at mRNA level were evaluated. Results: Acquired results showed that fluoxetine with and without AC and PKA inhibition resulted in Cx43 up-regulation both in mRNA and protein levels, whereas AQP4 expression have not changed. Discussion: In conclusion, data showed that fluoxetine alone and in the absence of serotonin acutely up-regulated Cx43 expression in astrocytes that can be assumed in molecular target therapy of MS patients. It seems that cAMP involvement in fluoxetine effects need more researches

Selective β2 adrenergic agonist increases Cx43 and miR-451 expression via cAMP-Epac

It has been demonstrated that connexin 43 (Cx43) and microRNAs have significant roles in glioma. Cyclic adenosine monophosphate (cAMP) is suggested to be a regulator of connexins and microRNAs. However, it remains elusive whether cAMP and exchange protein directly activated by cAMP (Epac2), have a regulatory effect on Cx43 and microRNA-451 (miR-451) in astrocytoma cells. We treated 1321N1 astrocytoma cells with a selective β2 adrenergic agonist and a selective Epac activator with and without adenyl cyclase and protein kinase A inhibition. Cx43 and miR-451 expression were measured. Next, we evaluated the effect of miR-451 overexpression on Cx43 expression. Cell proliferation was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results demonstrated that cAMPE-pac2 increased Cx43 and miR-451 expression. However, the alteration of miR-451 expression required a higher dose of drugs. Overexpression of miR-451 had no significant effect on Cx43 expression. The MTT assay showed that cAMP-Epac stimulation and miR-451 overexpression had a synergic inhibitory effect on cell proliferation. These findings may expand our understanding of the molecular biology of glioma and provide new potential therapeutic targets