The evaluation of antibacterial and antiproliferative activities of Rosmarinus officinalis essential oil from southern Morocco

Essential oils of plants contain many bioactive molecules and have recently a considerable place of application in pharmaceutical, cosmetic and food industry. The aim of this work is to study antibacterial and antiproliferative activities of Rosmarinus officinalis essential oil.Essential oil was extracted from dried plant by hydrodistillation, and then analyzed by gas chromatography-mass spectrometry (GC/MS). The antibacterial activity of the essential oil was tested by micro-atmosphere technique and agar diffusion against seven strains bacteria. The antiproliferative activity was determined by viability test against P3X63Ag8 myeloma cell in presence of various amount of essential oil. The morphological alterations were searched after staining of treated cells by le May-Grünwald-Giemsa.Our results showed that the major component detected in R. officinalis essential oil is 1.8-cineole (42%). This essential oil revealed an important antibacterial activity against resistant bacteria. The viability test showed a cytotoxic effect of essential oil on P3X63Ag8 myeloma cell. The morphological of the treated tumor cells analysis revealed a cellular lysis with membrane rupture, a hypertrophy and chromatin condensation.In conclusion, the R. officinalis essential oil could have an antibacterial activity against resistant bacteria and antiprolioferative activity against myeloma cells

COVID-19: Preventive knowledge and practices among Moroccan high school students

Coronavirus 2019 (COVID-19) has been recognized as a pandemic by the World Health Organization. Global efforts have been made to prevent the spread of the disease through political decisions and personal behaviors. In Morocco, a series of measures have been adopted to control the spread of the COVID-19 pandemic. This study was conducted to assess the knowledge and practices regarding COVID-19 among Moroccan high school students. All subjects were interviewed using an online questionnaire that tested their knowledge and preventive practices concerning COVID-19. Analysis of socio-demographic data showed that 56.33% were girls, aged between 15 and 18 years (85.3%), the majority was science students (95.3%), from urban areas of the Settat-Casablanca region. The survey revealed that social media was the main source of knowledge about the COVID-19, the majority of students (96%) surveyed high school students followed the information of the evolution of the COVID-19 pandemic, 97% of learners confirmed keeping the recommended social distance, while 68.60% actually practice this measure, 82.40% of high school students are aware of the persistence of COVID on surfaces, more than half of the participants do not shake hands greeting each other (56.40%)