Visualization of the action of ligninolytic enzymes on high yield pulp fibers

High-yield pulps from wheat straw and from poplar wood were treated with manganese-peroxidase (MnP) or with laccase (Lac), before and after a second refining stage

Получение на поверхности высокохромистых сталей карбидоупрочняемых диффузионных слоев заданного уровня свойств

The paper shows theoretical substantiated possibility to obtain high-quality carbide strengthening diffusion layers on the surface of high-chromium steel and the fields of their practical application.В статье представлена теоретически обоснованная возможность получения на поверхности высокохромистых сталей качественных карбидоупрочняемых диффузионных слоев, а также области их практического использования

REX-ISOLDE: post-accelerated radioactive BEAMS at CERN-ISOLDE

The ISOLDE RIB-facility at CERN has today been producing a vast range of radioactive beams since more than 30 years. The low-energy beams of ISOLDE will be complemented by a post-accelerator, REX-ISOLDE, currently being assembled. In order to convert the pseudo-DC, singly-charged beam from the ISOLDE mass separators into a cooled and bunched beam at higher charge states a novel scheme of trapping, cooling and charge-state breeding has been devised, using a linear Penning trap and an Electron Beam Ion Source (EBIS). This allows for subsequent acceleration by a short, cost-effective LINAC consisting of an RFQ, an IH-structure and three seven-gap resonators, reaching 0.8 - 2.2 MeV/u. The installation of REX-ISOLDE is well underway and the first post-accelerated radioactive beams are expected to be obtained during late 2000

Molecular cloning of the cDNA encoding laccase from Pycnoporus cinnabarinus I-937 and expression in Pichia pastoris

International audienc

Molecular cloning of the cDNA encoding laccase from Pycnoporus cinnabarinus I-937 and expression in Pichia pastoris

International audienc

Metabolic pathways of biotransformation and biosynthesis of aromatic compounds for the flavour industry by the basidiomycete Pycnoporus cinnabarinus

Among filamentous fungi, white-rot Basidiomycetes have become a strategic group to generate industrial aromatic flavours. In the course of a basidiomycete screening, the biotechnological potential of #Pycnoporus cinnabarinus strains was studied in order to produce, by transformation or de novo, natural aromatic flavours in liquid cultures. Ferulic acid and L-phenylalanine were found to be suitable substrates for vanillin and benzaldehyde (bitter almond aroma) production, respectively. These strains were also capable of producing de novo methylanthranilate, which has been described as the organoleptic note of wood strawberry. However, strains of #P. cinnabarinus often expressed laccase activity, which was unfavourable because the pathway to aromatic flavours is bypassed. To overcome this problem, the selection of monokaryotic laccase-deficient strains from basidiospores by classical genetics allowed to obtain more productive and more stable mycelial lines. (Résumé d'auteur

Spatial and temporal accumulation of mRNAs encoding two common lignin peroxidases in Phanerochaete chrysosporium.

Accumulation of peroxidases and their mRNAs was localized in colonies of Phanerochaete chrysosporium sandwiched between perforated polycarbonate membranes. Northern (RNA) blot analyses of colonial rings and in situ hybridizations with specific probes for manganese(II)-dependent peroxidase (MnP-1) and lignin peroxidase (LiP H8) mRNAs indicated that the expression of MnP-1 and Lip H8 genes started simultaneously in the central area of 3-day-old colonies. With time the signals for both transcripts spread to more-peripheral areas while decreasing in intensity. Furthermore, the appearance of MnP protein, as detected with specific immune serum, immediately followed accumulation of the MnP-1 mRNA transcript. However, LiP protein could be detected only some time after accumulation of LiP H8 mRNA

Obtaining of Carbide Strengthening Diffusion Layers of the Given Properties on the Surface of High-Chromium Steel

The paper shows theoretical substantiated possibility to obtain high-quality carbide strengthening diffusion layers on the surface of high-chromium steel and the fields of their practical application