Estrous cycle hormones in control (n = 9) and obese (n = 10) Ossabaw pigs.

<p>^# Significant difference between the control and obese pigs at the <i>p</i> <0.05 level during the follicular phase of the estrous cycle.</p><p>* Significant difference between the control and obese pigs at the <i>p</i> <0.05 level during the luteal phase of the estrous cycle.</p><p>Estrous cycle hormones in control (n = 9) and obese (n = 10) Ossabaw pigs.</p

Intravenous glucose tolerance test in control (n = 9) and obese (n = 9) Ossabaw pigs.

<p>Points on the obese pig glucose curve marked with an * are significantly greater than corresponding points on the control pig glucose curve (p<0.01). The insulin point on the obese pig curve marked with a # is significantly greater than the corresponding point on the control pig curve (p = 0.05). Obese pigs had greater HOMA-IR values at all post-glucose bolus points as compared with control pigs (p≤0.05).</p

Naïve granulosa and theca cell steroidogenic enzyme gene expression in control (n = 4) and obese (n = 6) Ossabaw pigs.

<p>Cells were collected immediately following euthanasia and follicle collection. Within a given gene, differences in relative fold induction/18s between control and obese pigs are shown with an * (<i>p</i><0.05). P4 = progesterone; DHEA = dehydroepiandrostereone; Δ4 = androstenedione; 17OHP4 = 17-alpha-hydroxy-progesterone; Test = testosterone; E2 = estradiol.</p

Theca cell androstenedione production in response to control (C), LH (LH; 10 ng/ml), insulin (I; 100 ng/ml) or LH + insulin (LI; 10 ng/ml + 100 ng/ml) treatments in vitro in control (n = 4) and obese (n = 6) pigs.

<p>Within a given cell culture treatment, significant differences between control and obese Ossabaw pigs are marked with an * (<i>p</i><0.05).</p

Abundance of phyla in colonic and cecal microbiota of lean and obese Göttingen minipigs (A–C).

<p>Different phyla in colon and cecum of lean and obese Göttingen minipigs. The error bars represent standard deviations.</p

Abundance of different bacterial families in colonic and cecal microbiota of lean and obese Göttingen minipigs.

<p>Different families in colon and cecum of lean and obese Göttingen minipigs. Parentheses indicate unknown family.</p

Fold-differences in the relative abundance of bacteria between lean and obese minipigs.

<p>Relative abundance of bacteria in different phylogenetic groups in cecal and colonic microbiota of obese Göttingen minipigs and in ileal and colonic microbiota of obese Ossabaw minipigs, estimated by qPCR dynamic array.</p><p>N.D. stands for Non Detectable.</p>*<p>indicates values with significant difference of <i>P<</i>0.05.</p

Abundance of different genera in colonic and ileal microbiota of lean and obese (MetS) Ossabaw minipigs.

<p>Selected genera in terminal ileum (TI) and colon of lean and obese Ossabaw minipigs. The unknown genera are indicated by parentheses belonging to the corresponding order or family.</p

Abundance of different genera in colonic and cecal microbiota of lean and obese Göttingen minipigs.

<p>Different genera in colon and cecum of lean and obese Göttingen minipigs. Parentheses indicates unknown genus belonging to an order or a family.</p

Blood parameters in lean and Obese Ossabaw and Göttingen minipigs.

<p>Blood parameters in lean and Obese Ossabaw and Göttingen minipigs.</p