Responses to hydric stress in the seed-borne necrotrophic fungus Alternaria brassicicola

Alternaria brassicicola is a necrotrophic fungus causing black spot disease and is an economically important seed-borne pathogen of cultivated brassicas. Seed transmission is a crucial component of its parasitic cycle as it promotes long-term survival and dispersal. Recent studies, conducted with the Arabidopsis thaliana/A. brassicicola pathosystem, showed that the level of susceptibility of the fungus to water stress strongly influenced its seed transmission ability. In this study, we gained further insights into the mechanisms involved in the seed infection process by analyzing the transcriptomic and metabolomic responses of germinated spores of A. brassicicola exposed to water stress. Then, the repertoire of putative hydrophilins, a group of proteins that are assumed to be involved in cellular dehydration tolerance, was established in A. brassicicola based on the expression data and additional structural and biochemical criteria. Phenotyping of single deletion mutants deficient for fungal hydrophilin-like proteins showed that they were affected in their transmission to A. thaliana seeds, although their aggressiveness on host vegetative tissues remained intact

La resistance des moustiques aux insecticides

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Turnip yellow mosaic virus RNA-replicase contains host and virus encoded subunits

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Characterization of two plasmids from Spiroplasma citri strains MH and M4 : their distribution among other spiroplasma strains or species

International audienc

Characterization of two cryptic plasmids from Spiroplasma citri and occurrence of their DNA sequences among various spiroplasmas

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Spiroplasmas and the transfer of genetic material by transformation and transfection

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Spot hybridization : application to viroid identification

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Immunodetection de proteines non structurales du virus de la mosaique jaune du navet dans les feuilles de chou chinois et dans les protoplastes infectes [replicase, immunotransfert; expression genomique

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Variation and temporal patterns in the composition of the surface ichthyoplankton in the southern Bay of Biscay (W. Atlantic)

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Expression of the Spiroplasma citri spiralin gene in Escherichia coli. Use of the recombinant plasmid carrying the gene as a molecular probe

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