Role of aflatoxin B1 on hematological factors, histopathological changes, tissue residual, growth and survival rate of Fenneropenaeus indicus

Role of aflatoxin B1 on hematological indexes, histopathological changes, tissue residual, growth and survival rate of Fenneropenaeus indicus Some similar cases of aflatoxin chronic poisoning were reported from cultured shrimps in Iran. In this study, Indian white shrimp, Fenneropenaeus indicus, with a weight of 11.79 ± 1.76 grams were fed with diets containing 0, 20, 50, 100, 200, 400, 800 and 1600 ppb levels of AFB1 for 8 weeks. Growth performances, survival rate, residue in muscles and cephalothorax, hematological and histopathological changes were determined after 4 and 8 weeks. The results showed that after 8 week of feeding shrimps with values of 20, 50 and 100 ppb aflatoxin B1, did not affect the growth rate, but with increased levels of Aflatoxin B1 in feed, the growth is reduced and the correlation (r) minus the final weight equal to - 0.312. After eight weeks doses of AFB1 in the diets showed negative correlation to the survival rate, total haemocyte count (THC), total plasma protein (TPP) equal to -0.603, -0.237 and -0.649 respectively. Histopathological changes were variety necrosis, degeneration and atrophy of epithelial and secretory cells lining the tubules, infiltration of fibroblasts in the walls of hepatopancreas tubules, separated muscle bundles in Muscle tissues and intestinal mucosal necrosis and detachment. Possible amelioration was examined by feeding control and treated shrimp (after eight weeks exposure to AFB1) by dietary without AFB1 for four weeks. The Recovery was observed in all parameters of 20, 50, 100 and 200 ppb AFB1 treatments but shrimps fed with the 1600 ppb and 800 ppb concentrations of AFB1 exhibited slower growth, more reddish discoloration disseminated over the body and lack of consistency in the hepatopancreas at 4th week recovery period. After eight weeks, the highest level of aflatoxin B1 residues in shrimp muscles was observed in 100 ppb treatment that its value was 1.55 ppb, but the highest level of aflatoxin B1 residues in cephalothorax was observed in 1600 ppb treatment that its value was 7.1 ppb. In terms of public health, these amounts of aflatoxin in shrimp body are less than permissible limit for human consumption

Supply and protected different population of Litopenaeus vannamei subadult zero foster (F0) from difference provinces Iran

Nowadays, Litopenaeus vannamei are the most important species of farmed penaeidae shrimp in the world that is rapidly replacing native species in areas aquaculture. Due to demand increase for this species culture, shrimp displacement to different areas may be associated with some potential pathogens transferred to new areas farmed. Therefore, in this study were prepared bi-osecurity conditions for specific disease-free production of L. vannamei. Thereafter, three populations (Molokaei, High health and mix of Molokaei and High health) of the shrimp various reserves were detected base on origin and genetic indexes such as: observed heterozygosity, expected heterozygosity, allele frequency, coefficient inbreeding, genetic differentiation, genetic distance and genetic identity. On the other hand, epidemiological studies indicate non pathogens (viral, bacterial, fungal and parasitic) recognition of different populations selected in the quarantine salon. The bioassay results showed that the average weight and length of the populations of High health and Mix significantly greater than was a population of Molokaei. The shrimp populations were stocked in fiberglass tank (five ton) and were kept separated in the quarantine salon. During maintenance shrimp of populations in the quarantine salon were evaluated living and non-living pathogens with PCR, microbiology and biochemical methods. There is not any pathogens detection from shrimp populations stocking in the quarantine salon, so the shrimps were carried over to pond for broodstock culture of specific pathogenic free