Macrophage migration inhibitory factor (MIF) expression of non-small cell lung cancer (NSCLC) cell lines and the effects of miR-451 mimic transfection of NSCLC cell lines.

<p>(A) MIF expression in NSCLC cell lines by western blotting. H441 and H1975 showed relatively higher MIF expression. (B) Western blotting of MIF, phosphorylated Akt (pAkt), total Akt, phosphorylated Erk (pErk), and total Erk of H441 and H1975 cells after miR-451-mimic (miR-451) and Mimic Negative Control (control) transfection. (C) Cell proliferation assay of H441 and H1975 cells after miR-451-mimic (miR-451) and Mimic Negative Control (control) transfection d: day, error bars: +/-SEM, *: <i>P</i><0.05 (unpaired t-test), **: <i>P</i> <0.005 (unpaired t-test). (D) Cell migration assay of H1975 cells after miR-451-mimic (miR-451) and Mimic Negative Control (control) transfection. Migrated cell stained with Giemsa solution (left) and migrated cell counts (right). Error bars: +/-SEM, **:<i>P</i><0.005 (unpaired t-test).</p

miR-451 expression in paired background lung and non-small cell lung cancer tissues.

<p>The miR-451 expression is significantly lower in NSCLC tissues than in background lung tissue (<i>P</i><0.0001 with paired t-test). Y-axis: -δCt = - -(Ct miR-451−Ct RNU6B).</p

A univariate analysis of clinicopathological factors for a disease-free survival in non-small cell lung cancer patients.

<p>A univariate analysis of clinicopathological factors for a disease-free survival in non-small cell lung cancer patients.</p

The macrophage migration inhibitory factor (MIF) expression of non-small cell lung cancers.

<p>Representative adenocarcinoma (a) and squamous cell carcinoma (b) cases with a high expression of MIF (objective x20). The cases were scored as follows: (a) Proportion score (PS), 3; intensity score (IS), 3 and total score (TS), 6. (b) PS, 3; IS, 2 and TS, 5. An adenocarcinoma case negative for MIF expression (TS, 0) is shown in (c).</p

A univariate analysis of clinicopathological factors for a disease-free survival in non-small cell lung cancer patients.

<p>A univariate analysis of clinicopathological factors for a disease-free survival in non-small cell lung cancer patients.</p

A multivariate survival analysis in lung adenocarcinoma cases.

<p>A multivariate survival analysis in lung adenocarcinoma cases.</p

miR-451 and the disease-free survival (DFS) in non-small cell lung cancer (NSCLC) and lung adenocarcinoma cases.

<p>(A) NSCLC cases with a low miR-451 expression showed a significantly worse prognosis than cases with a high miR-451 expression. (B) Lung adenocarcinoma cases with a low miR-451 expression showed a significantly worse prognosis than cases with a high miR-451 expression. Tick: censored cases.</p

Correlations of the miR-451 expression with clinicopathological factors in non-small cell lung carcinomas.

<p>Correlations of the miR-451 expression with clinicopathological factors in non-small cell lung carcinomas.</p

A multivariate survival analysis in non-small cell lung cancer cases.

<p>A multivariate survival analysis in non-small cell lung cancer cases.</p

Correlations of the miR-451 expression with clinicopathological factors in lung adenocarcinomas.

<p>Correlations of the miR-451 expression with clinicopathological factors in lung adenocarcinomas.</p