34 research outputs found
The rice OsERF101 transcription factor regulates the NLR Xa1-mediated immunity induced by perception of TAL effectors
イネが病原菌の感染力の源を検出して免疫を誘導する仕組みを解明 --病気に強い植物の開発に期待--. 京都大学プレスリリース. 2022-09-07.Plant nucleotide-binding leucine-rich repeat receptors (NLRs) initiate immune responses by recognizing pathogen effectors. The rice gene Xa1 encodes an NLR with an N-terminal BED domain, and recognizes transcription activator-like (TAL) effectors of Xanthomonas oryzae pv. oryzae (Xoo). Our goal is to elucidate the molecular mechanisms controlling the induction of immunity by Xa1. We used yeast two-hybrid assays to screen for host factors that interact with Xa1 and identified the AP2/ERF-type transcription factor OsERF101/OsRAP2.6. Molecular complementation assays were used to confirm the interactions among Xa1, OsERF101, and two TAL effectors. We created OsERF101-overexpressing and knockout mutant lines in rice and identified genes differentially regulated in these lines, many of which are predicted to be involved in regulation of response to stimulus. Xa1 interacts in the nucleus with the TAL effectors and OsERF101 via the BED domain. Unexpectedly, both the overexpression and knockout lines of OsERF101 displayed Xa1-dependent, enhanced resistance to an incompatible Xoo strain. Different sets of genes were up- or down-regulated in the overexpression and knockout lines. Our results indicate that OsERF101 regulates the recognition of TAL effectors by Xa1, and functions as a positive regulator of Xa1-mediated immunity. Further, an additional Xa1-mediated immune pathway is negatively regulated by OsERF101
KamLAND Sensitivity to Neutrinos from Pre-Supernova Stars
In the late stages of nuclear burning for massive stars (M>8~M_{\sun}), the
production of neutrino-antineutrino pairs through various processes becomes the
dominant stellar cooling mechanism. As the star evolves, the energy of these
neutrinos increases and in the days preceding the supernova a significant
fraction of emitted electron anti-neutrinos exceeds the energy threshold for
inverse beta decay on free hydrogen. This is the golden channel for liquid
scintillator detectors because the coincidence signature allows for significant
reductions in background signals. We find that the kiloton-scale liquid
scintillator detector KamLAND can detect these pre-supernova neutrinos from a
star with a mass of 25~M_{\sun} at a distance less than 690~pc with 3
significance before the supernova. This limit is dependent on the neutrino mass
ordering and background levels. KamLAND takes data continuously and can provide
a supernova alert to the community.Comment: 19 pages, 6 figures, 1 tabl
KamLAND SENSITIVITY TO NEUTRINOS FROM PRE-SUPERNOVA STARS
In the late stages of nuclear burning for massive stars (M > 8 M ⊙), the production of neutrino-antineutrino pairs through various processes becomes the dominant stellar cooling mechanism. Furthermore, as the star evolves, the energy of these neutrinos increases and in the days preceding the supernova a significant fraction of emitted electron anti-neutrinos exceeds the energy threshold for inverse beta decay on free hydrogen. This is the golden channel for liquid scintillator detectors because the coincidence signature allows for significant reductions in background signals. Here, we find that the kiloton-scale liquid scintillator detector KamLAND can detect these pre-supernova neutrinos from a star with a mass of 25 M ⊙ at a distance less than 690 pc with 3σ significance before the supernova. This limit is dependent on the neutrino mass ordering and background levels. KamLAND takes data continuously and can provide a supernova alert to the community
White paper: CeLAND - Investigation of the reactor antineutrino anomaly with an intense 144Ce-144Pr antineutrino source in KamLAND
We propose to test for short baseline neutrino oscillations, implied by the
recent reevaluation of the reactor antineutrino flux and by anomalous results
from the gallium solar neutrino detectors. The test will consist of producing a
75 kCi 144Ce - 144Pr antineutrino source to be deployed in the Kamioka Liquid
Scintillator Anti-Neutrino Detector (KamLAND). KamLAND's 13m diameter target
volume provides a suitable environment to measure energy and position
dependence of the detected neutrino flux. A characteristic oscillation pattern
would be visible for a baseline of about 10 m or less, providing a very clean
signal of neutrino disappearance into a yet-unknown, "sterile" state. Such a
measurement will be free of any reactor-related uncertainties. After 1.5 years
of data taking the Reactor Antineutrino Anomaly parameter space will be tested
at > 95% C.L.Comment: White paper prepared for Snowmass-2013; slightly different author
lis
CeLAND: search for a 4th light neutrino state with a 3 PBq 144Ce-144Pr electron antineutrino generator in KamLAND
The reactor neutrino and gallium anomalies can be tested with a 3-4 PBq
(75-100 kCi scale) 144Ce-144Pr antineutrino beta-source deployed at the center
or next to a large low-background liquid scintillator detector. The
antineutrino generator will be produced by the Russian reprocessing plant PA
Mayak as early as 2014, transported to Japan, and deployed in the Kamioka
Liquid Scintillator Anti-Neutrino Detector (KamLAND) as early as 2015.
KamLAND's 13 m diameter target volume provides a suitable environment to
measure the energy and position dependence of the detected neutrino flux. A
characteristic oscillation pattern would be visible for a baseline of about 10
m or less, providing a very clean signal of neutrino disappearance into a
yet-unknown, sterile neutrino state. This will provide a comprehensive test of
the electron dissaperance neutrino anomalies and could lead to the discovery of
a 4th neutrino state for Delta_m^2 > 0.1 eV^2 and sin^2(2theta) > 0.05.Comment: 67 pages, 50 figures. Th. Lasserre thanks the European Research
Council for support under the Starting Grant StG-30718
Search for the Invisible Decay of Neutrons with KamLAND
The Kamioka Liquid scintillator Anti-Neutrino Detector (KamLAND) is used in a
search for single neutron or two neutron intra-nuclear disappearance that would
produce holes in the -shell energy level of C nuclei. Such holes
could be created as a result of nucleon decay into invisible modes (),
e.g. or . The de-excitation of the corresponding
daughter nucleus results in a sequence of space and time correlated events
observable in the liquid scintillator detector. We report on new limits for
one- and two-neutron disappearance: years
and years at 90% CL. These results
represent an improvement of factors of 3 and over previous
experiments.Comment: 5 pages, 3 figure
Measurement of Neutrino Oscillation with KamLAND: Evidence of Spectral Distortion
We present results of a study of neutrino oscillation based on a 766 ton-year
exposure of KamLAND to reactor anti-neutrinos. We observe 258 \nuebar\
candidate events with energies above 3.4 MeV compared to 365.2 events expected
in the absence of neutrino oscillation. Accounting for 17.8 expected background
events, the statistical significance for reactor \nuebar disappearance is
99.998%. The observed energy spectrum disagrees with the expected spectral
shape in the absence of neutrino oscillation at 99.6% significance and prefers
the distortion expected from \nuebar oscillation effects. A two-neutrino
oscillation analysis of the KamLAND data gives \DeltaMSq =
7.9 eV. A global analysis of data from KamLAND
and solar neutrino experiments yields \DeltaMSq =
7.9 eV and \ThetaParam =
0.40, the most precise determination to date.Comment: 5 pages, 4 figures; submitted to Phys.Rev.Letter
First Results from KamLAND: Evidence for Reactor Anti-Neutrino Disappearance
KamLAND has been used to measure the flux of 's from distant
nuclear reactors. In an exposure of 162 tonyr (145.1 days) the ratio of
the number of observed inverse -decay events to the expected number of
events without disappearance is for energies 3.4 MeV. The deficit of events is
inconsistent with the expected rate for standard propagation at
the 99.95% confidence level. In the context of two-flavor neutrino oscillations
with CPT invariance, these results exclude all oscillation solutions but the
`Large Mixing Angle' solution to the solar neutrino problem using reactor
sources.Comment: 6 pages, 6 figure