LPS pretreatment suppressed CNV formation.

<p>Peritoneal injection of low-dose LPS (20 µg) was performed at 4, 3, 2 or 1 days (respectively, Day -4, -3, -2 and -1) before laser irradiation (Day 0), or at 2 days after laser irradiation (Day +2), and the CNV size was evaluated at 10 days after laser treatment (Day 10) (A). In all groups of LPS-pretreated mice, the size of CNV was significantly smaller than that in control mice (B, C). The smallest CNV was shown in the mice given LPS pretreatment 2 days before laser treatment. The bars show means ± SEM. <i>n</i> = 6 mice/group, *<i>P</i> = 0.002 compared with control.</p

Risk factors for recurrence of RD in the subgroup of inferior breaks.

<p>Risk factors for recurrence of RD in the subgroup of inferior breaks.</p

Postoperative outcome measures after vitrectomy with prone positioning versus without prone positioning.

<p>Postoperative outcome measures after vitrectomy with prone positioning versus without prone positioning.</p

Adoptive transfer of LPS-treated peritoneal macrophages suppressed CNV formation as well as did LPS pretreatment.

<p>The donor mice were injected with LPS (20 µg/PBS 200 µl) or PBS (200 µl) at Day -2. At Day 0, laser treatment was performed on the recipient mice. After that, peritoneal macrophages were harvested from the donor mice and 2×10⁶ or 1×10⁶ macrophages were transferred into the peritoneal cavity of the recipient mice (A). For comparison, laser treatment was performed in PBS-pretreated mice and LPS-pretreated mice without adoptive transfer. In the LPS-pretreated mice and the recipient mice with 2×10⁶ macrophages from LPS-treated donor mice, CNV was significantly smaller than that in the control mice and the recipient mice with macrophages from PBS-pretreated donor mice (B). The bars show means ± SEM. <i>n</i> = 6 mice/group, *<i>P</i> = 0.003 compared with control.</p

Subgroup analysis of baseline characteristics in patients with inferior breaks.

<p>Subgroup analysis of baseline characteristics in patients with inferior breaks.</p

Baseline patient characteristics with prone positioning versus no prone positioning.

<p>Baseline patient characteristics with prone positioning versus no prone positioning.</p

Vitrectomy without prone positioning for rhegmatogenous retinal detachments in eyes with inferior retinal breaks

<div><p>Purpose</p><p>To compare the anatomic and functional outcomes of pars plana vitrectomy (PPV) for treating rhegmatogenous retinal detachments (RRDs) between two groups with and without postoperative prone positioning.</p><p>Methods</p><p>This retrospective cohort study included 142 eyes of 142 patients with a primary RRD. All patients underwent PPV with 20% sulfur hexafluoride gas tamponade and were divided into two groups: the groups that did and did not maintain a prone position postoperatively. All patients were followed for more than 3 months. The main outcome measures were the best-corrected visual acuity (BCVA), retinal reattachment rate, and postoperative complications.</p><p>Results</p><p>Sixty-five eyes were included in the prone position group and 77 eyes in the group without prone positioning; the respective initial reattachment rates were 83.1% and 96.1%, a difference that reach significance (p = 0.011). In the eyes with inferior breaks, the initial reattachment rate was 94.7% (18 eyes) without prone positioning, which was significantly (p = 0.036) better than the 60% (6 eyes) initial reattachment rate in the group with prone positioning. In the eyes without inferior breaks, there was no significant difference in the initial reattachment rates between the two groups. The BCVAs at the 3-month postoperative visit did not differ significantly between the two groups. An epiretinal membrane (ERM) was observed postoperatively in 10 (13.0%) eyes in the group without prone positioning; no ERMs were seen postoperatively in eyes in which the internal limiting membrane (ILM) was peeled during PPV.</p><p>Conclusions</p><p>PPV without postoperative prone positioning is associated with a higher reattachment rate in eyes with a RRD, especially those with inferior retinal breaks. PPV with postoperative supine and lateral positioning might be beneficial to manage RRDs associated with inferior retinal breaks if ILM peeling is performed intraoperatively.</p></div

Anti-IL-10 antibody inhibited the CNV inhibitory effect of LPS pretreatment.

<p>Peritoneal injection of anti-IL-10 neutralizing antibody (IL-10Ab) inhibited the CNV inhibitory effect of LPS pretreatment in LPS-treated mice. The bars show means ± SEM. <i>n</i> = 6 mice/group, *<i>P</i> = 0.01.</p

Outcome measures after prone positioning following vitrectomy compared with no prone positioning in eyes that underwent phacovitrectomy.

<p>Outcome measures after prone positioning following vitrectomy compared with no prone positioning in eyes that underwent phacovitrectomy.</p

LPS treatment increased serum IL-10 concentration and IL-10 expression in peritoneal macrophages and in the eye.

<p>After peritoneal injection of low-dose LPS, IL-10 expression in the peritoneal macrophages (A) and in the posterior part of the eye (the retina, RPE and choroid) (B) increased, approximately 8-fold and 4-fold, respectively, two days after LPS injection. The bars show means ± SEM. <i>n</i> = 6 mice/group, *<i>P</i><0.001 compared with control. Serum IL-10 concentration increased (C). <i>n</i> = 6, *<i>P</i><0.001 compared with baseline. It reached a peak on day 1 and gradually decreased. A significant increase was shown for at least 4 days.</p