Factor analysis of body measurements of local cows of Manipur, India

Eighteen different biometric traits in 250 local cows of Manipur from their breeding zone, i.e. Imphal valley of Manipur, India, were recorded and analyzed by principal component analysis to explain body conformation. The averages of height at withers (HW), body length, heart girth, paunch girth, forehead width, ear length, tail length, switch length, neck circumference, neck length, arm length,  elbow length, fore-shank length, thigh length, hind-shank length, pes length, head length and eye to eye space were 103.92±0.33, 111.34±0.92, 135.34±0.47, 140.31±0.53, 14.90±0.15, 15.24±0.13, 75.50±0.55, 31.04±0.24, 58.61±0.53, 29.95±0.21, 29.34±0.19, 29.88±0.17, 29.59±0.20, 30.32±0.20, 29.88±0.13, 31.65±0.25, 37.30±0.25 and 26.47±0.20 cm, respectively. The correlation coefficients ranged from -0.20 (hind shank length and eye to eye width) to 0.74 (heart girth and paunch girth).  Factor analysis with promax rotation revealed seven factors which explained about 64.31% of the total variation. Factor 1 described the general body conformation and explained 17.74% of total variation. It was represented by significant positive high loading of height at wither, heart girth, paunch girth and ear length. The remaining factors described 11.71%, 8.88%, 7.47%, 6.60%, 6.04% and 5.86% of total variability.  It was necessary to include some more variables for a reliable analysis of factors as there were less than three variables except the first factor in the present study. The communality ranged from 0.493 (elbow length) to 0.782 (neck circumference) and unique factors ranged from 0.507 to 0.218 for all these 18 different biometric traits. The lower communalities for some of the traits like ear length, tail length, arm length, elbow length and thigh length might indicate that these traits were less effective to account for total variation of body conformation as compared to the other traits in local cows of Manipur. The result suggests that principal component analysis (PCA) could be used in breeding programs with a drastic reduction in the number of biometric traits to be recorded to explain body conformation

STUDIES ON INCIDENCE AND ANTIBIOGRAM OF SALMONELLA SEROVARS ISOLATED FROM RAW PORK IN AIZAWL AND IMPHAL, INDIA

The study was conducted to determine the incidence, serotypes and antimicrobial resistance profile of Salmonella serovars isolated from raw pork of Aizawl and Imphal, India. A total of 200 raw pork samples (100 from Aizawl and Imphal each) were collected from unorganized butcher shops and subjected for isolation and identification of Salmonella spp. which phenotypically detected 5 Salmonella isolates. The Salmonella isolates were further confirmed genotypically by 16S rRNA genus specific PCR and recorded the incidence rate of 2.50%. Serotyping of the isolates revealed that all the three isolates from Imphal were Salmonella enterica serovar Virchow whereas the two isolates of Aizawl were Salmonella enterica serovar Virchow and Salmonella enterica serovar Typhimurium, one each. The antimicrobial sensitivity profile against 16 antimicrobials revealed that amikacin, imipenem, ofloxacine, norfloxacin and ciprofloxacin were 100% sensitive and among the resistant antimicrobials, highest resistance was recorded against ceftriaxone (80.00%) followed by amoxyclav and cotrimoxazole (60.00% each). Presence of Salmonella Typhimurium from raw pork is a matter of concern from hygiene and sanitation. Moreover, the antimicrobial resistance profile showed increasing resistance against cephalosporin, amino-penicillin and amino glycosides

Effect of culture conditions on the growth of biomass Yarrowia lipolytica - producing protein feed

Fodder yeast is highly valuable protein-vitamin products. Protein digestibility by yeast and amino acid content, superior proteins of animal origin. Fodder yeast protein digested in animals by 95 %. The biological value of yeast protein is determined by the presence of a significant amount of essential amino acids. Moreover, yeast cells contain many vitamins microelement and a significant amount of fat, in which the predominant unsaturated fatty acid. Currently, fodder yeast successfully used in livestock and poultry, so the demand for them is increasing every year. For the production of fodder yeast using a yeast having the necessary technological properties: the ability of rapid growth in aerobic conditions to form protein, amino acids and vitamins, resistant crop production, the development of resistance to foreign microorganisms. Intensive education yeast biomass contributes to a number of conditions, including pH, temperature and aeration of the culture occupy an important place. The main criterion for comparison and selection of a culture medium for this is the speed of its growth and ability to assimilate all of the nutrients with high economic factor. It depends on the performance of the enterprise, energy consumption and other technical - economic performance. The effect of pH of the medium on the biomass accumulation of yeast Yarrowia lipolytica. Found that at pH 5,2 - 5,5 observed maximum growth rate of the yeast cells. The effect of temperature on the accumulation of yeast biomass. The temperature of the culture medium determines the intensity of metabolism in cells. It was found that the optimal growth temperature of the culture Yarrowia lipolytica is 33 0C. The effect of aeration on the growth rate of yeast cells. Tro-established that the maximum increase of biomass was obtained with the aeration of 70 cm3 /cm3hrs

Production of nano-bentonite and the study of its effect on mutagenesis in bacteria Salmonella typhimurium

© 2016, Pleiades Publishing, Ltd.The production of nano-bentonite and its effects on mutation process in the strains of Salmonella typhimurium are studied. It is revealed that nano-bentonite particles essentially differ from bentonite particles in structure, size, and shape. Bentonite particles are cone-shaped and 0.3 to 1.0 μm in size, whereas nanobentonite nanoparticles are oval-shaped and 25 to 95 μm in size. Single particles (less than 10.0%) are irregular polyhedra and 0.6 μm in size. The structure of bentonite consists of separate fragments of constituent minerals composed of packages–lamelee 0.6 μm in size cemented with an amorphous mass. An amorphous mass containing single micrometer-sized packages–lamelee is observed in the structure of the nano-bentonite. It is determined that nano-bentonite does not possess mutagenic activity on microorganisms. The study of antimutagenic potential of nano-bentonite reveals that it possesses a moderate inhibitory effect on mutagenesis caused by mitomycin C, 2,4-dinitrophenylhydrazine, and ethyl methanesulphonate, but does not inhibit genotoxic potential of hydrogen peroxide. The results demonstrate that nano-bentonite is nongenotoxic and can be used for the development of next-generation safe nanotechnological materials

Reforming Labor Legislation of the Belarus Republic: some problems

Several directions of reforming the Belarusian labor legislation are considered in this article, taking into account so urgent politics of flexicurity. For that reason causes of giving flexibility to legal regulation of employment agreement termination are explored. The conclusion is made about the necessity of taking into account the objective criteria of differentiation by determining the specifics of separate employers’ categories in the situation of labor relations terminatio

Preparation of biomass starter cultures for biotechnological рroductions

Studying of preparation methods influence for beet shavings and waters to the extraction process with application of different reagents (aluminum sulfate, bentonite and gypsum) to produce the electrochemically activated solutions on quality indicators of sugar beet produced juice

Application financial analysis methods for the designing of innovative food enterprises, aimed at import substitution

The article formulates the problem of the lack of pectin production in the country, which affects the food security of Russia. The relevance and significance of this problem, as well as the feasibility of creating a pectin production line in the territory of the Voronezh Region are considered. Various technologies for the production of pectin are given, but the most progressive is the method of electro-membrane processing of technological media. Further, the work presents the production and financial plan of the projected enterprise for the production of pectin and based on its results, forward-looking financial statements are formed. To assess the probability of bankruptcy of an enterprise, it is necessary to create an adapted methodology for assessing the risk of bankruptcy. On the basis of a sample of enterprises whose activities are related to the production of thickeners and gelling agents, some of which are actual bankrupts, a modified Suyazova model is created. economic profitability indicators, current liquidity ratio, return on equity, stock turnover ratio, asset turnover and product sales profitability across all enterprises in the last three years of the company's activity were calculated. On the basis of the calculations made, a new method was obtained, defining 4 possible probabilities of bankruptcy: high, medium, low and minimal. The technique was tested on the same enterprises that were used to create it. Approbation of the methodology showed that in the year of bankruptcy all bankrupt enterprises were classified as bankrupt, and the existing ones were recognized as valid. The model also predicts the average and high probability of bankruptcy two years before the onset of insolvency. This allows us to conclude that the predictive power of the proposed methodology is high. The created model predicts minimal bankruptcy risk for the projected enterprise. Consequently, the creation of pectin production is recognized as expedient and the development of this enterprise must continue

STUDY OF NEPETAE CATARIAE HERBA FRUITS AS PROMISING MEDICINAL PLANT RAW MATERIAL

Nepetae catariae herba is used in the Russian Federation as spice. The chemical compounds of herb have been studied. This plant has been introduced into the culture. There are its domestic varieties. The fruits of the plant accumulate up to 25% of fatty oil and contain specific laballenic acid, which has a wide range of antimicrobial and fungicidal actions. The yield of fruit crops is 5–6 c/ha.The aim is to study some criteria for the standardization of the quality of Nepetae catariae herb as a new medicinal plant raw material.Materials and methods. The studied fruits are from the biological collection of Federal State Budgetary Institution of All – Russia Research Institute of Medicinal and Aromatic Plants. Binocular magnifier brand MBS-10 and Axioplan 2 imaging microscope by Carl Zeiss were used. The sample preparation was carried out according to the State Pharmacopoeia of the Russian Federation (XIV edition). Anatomical diagnostic features were studied in powder and cross section with staining reagents for the presence of some biologically active substances. Qualitative reactions were carried out with water and alcohol-water extracts from the fruits. The content of the lipid complex according to pharmacopoeia monograph 2.5.0035.15 has been determined.Results. A description of the external and diagnostic anatomical features is given. The main groups of biologically active substances and the content of the lipid complex in a possible new material – the fruits of Nepeta cataria – have been identified.Conclusion. The description of the external features of the fruit has been specified. For the first time, crushed fruits have been characterized. It has been established that the morphology of endocarp cells and seed embryo cells are best preserved in a mellow fruit. Physico-optical properties of cellular structures and the ability for basic microchemical reactions are preserved in all zones of pericarp and seeds. Qualitative reactions showed the presence of the following components in the fruits: saponins, flavonoids and a lipid complex. A dispersion composition has been studied. The yield of the lipid complex and its appearance have been determined. Fruits can be used as promising fat-oil raw materials. The results of the study can be used in drafting Pharmacovigilance Reference Document considering a promising type of medicinal plant raw material on the basis of Nepetae catariae herba fruits

INVESTIGATION OF PREBIOTIC, IMMUNOSTIMULATING PROPERTIES OF FUCOSE AND ITS EFFECT ON REPRODUCTIVE FUNCTION

Aim. Investigation of the effect of fucose in the diet on the gastrointestinal microflora of experimental animals with experimental dysbiosis, the humoral factors of nonspecific immunity, as well as the degree of fucosylation of oocytes and the proportion of oocytes that can be fertilized. Materials and methods. Prebiotic properties of fucose were studied by analyzing the luminal microflora of experimental mice against the background of experimental dysbiosis. Investigation of factors of nonspecific immunity was carried out after immunization of mice according to the level of antibody formation in blood serum by the method of enzyme immunoassay. The degree of fucosylation of oocytes was assessed by the intensity of their luminescence upon microscopy of oocytes of experimental mice on a fluorescent microscope. Results. The use of fucose in all tested doses led to the restoration of the composition and quantity of the gastrointestinal microflora. For the correction of dysbiosis, the optimal concentration of fucose was 0.02% of the body weight of the experimental animals. Inclusion of fucose in a diet of experimental animals in the amount of 0.008% to the body weight provided the highest level of immune response. The degree of fucosylation of oocytes, the proportion of oocytes capable of fertilization was increased when fucose were introduced in the amount of 0.008% to the body weight of the mice. Conclusion. Bifidogenic and lactogenic activity of fucose is established. The ability of fucose to stimulate an increase in the level of antibodies in in blood serum is shown. The tendency of positive effect of fucose in the diet of mice on the degree of fucosylation of oocytes was revealed

Production of nano-bentonite and the study of its effect on mutagenesis in bacteria Salmonella typhimurium

© 2016, Pleiades Publishing, Ltd.The production of nano-bentonite and its effects on mutation process in the strains of Salmonella typhimurium are studied. It is revealed that nano-bentonite particles essentially differ from bentonite particles in structure, size, and shape. Bentonite particles are cone-shaped and 0.3 to 1.0 μm in size, whereas nanobentonite nanoparticles are oval-shaped and 25 to 95 μm in size. Single particles (less than 10.0%) are irregular polyhedra and 0.6 μm in size. The structure of bentonite consists of separate fragments of constituent minerals composed of packages–lamelee 0.6 μm in size cemented with an amorphous mass. An amorphous mass containing single micrometer-sized packages–lamelee is observed in the structure of the nano-bentonite. It is determined that nano-bentonite does not possess mutagenic activity on microorganisms. The study of antimutagenic potential of nano-bentonite reveals that it possesses a moderate inhibitory effect on mutagenesis caused by mitomycin C, 2,4-dinitrophenylhydrazine, and ethyl methanesulphonate, but does not inhibit genotoxic potential of hydrogen peroxide. The results demonstrate that nano-bentonite is nongenotoxic and can be used for the development of next-generation safe nanotechnological materials