Novel anticancer drug delivery system based on zeolite encapsulating Hamelia patens leaf and flower extracts

181-194Nanotechnology had placed an impact in improving the antiproliferative activity of crude leaf (CL) and flower (CF) extracts of Hamelia patens on the liver (HEPG2) and breast (MCF7) carcinoma cell lines, after their encapsulation onto zeolite (ZSM-5) nanopores, which acts as a drug delivery system (DDS). The cytotoxicity of the new formulas showed remarkable improvement in the activities on HEPG2. CL had low activity on HEPG2, at the tested concentrations, but the ZSM-5 loaded one (CLZ) showed enhanced action with IC50=42 μg/mL. Also, the cytotoxicity of CF improved after Zeolite incorporation, where the IC50 has been changed from 48 μg/mL for CF to 25 μg/mL for CFZ. Moreover, the polar fraction of leaves extracted by methanol (Me.L) showed improvement on the cytotoxicity on MCF7 for zeolite loaded formula (Me.Z) (IC50 had changed from 65 to 44.4 μg/mL). Quantitative estimations for polyphenolic contents as well as the phenolic profiles for CF and CL extracts were inspected using HPLC-DAD to explore the bioactive phytochemical compounds. Acute toxicity test showed that LD50 was 1500 and 2333 mg/kg b.wt. for CL and CF, respectively. The cytogenetic study was also done to detect the chromosome aberrations in somatic and germ cells after CL and CF administration to mice. DPPH antioxidant capacity assay revealed that CL has high redox-active effect than CF. This study is a contribution to the development of a creative new DDS that help in cancer treatment

Novel anticancer drug delivery system based on zeolite encapsulating Hamelia patens leaf and flower extracts

Nanotechnology had placed an impact in improving the antiproliferative activity of crude leaf (CL) and flower (CF) extracts of Hamelia patens on the liver (HEPG2) and breast (MCF7) carcinoma cell lines, after their encapsulation onto zeolite (ZSM-5) nanopores, which acts as a drug delivery system (DDS). The cytotoxicity of the new formulas showed remarkable improvement in the activities on HEPG2. CL had low activity on HEPG2, at the tested concentrations, but the ZSM-5 loaded one (CLZ) showed enhanced action with IC50=42 μg/mL. Also, the cytotoxicity of CF improved after Zeolite incorporation, where the IC50 has been changed from 48 μg/mL for CF to 25 μg/mL for CFZ. Moreover, the polar fraction of leaves extracted by methanol (Me.L) showed improvement on the cytotoxicity on MCF7 for zeolite loaded formula (Me.Z) (IC50 had changed from 65 to 44.4 μg/mL). Quantitative estimations for polyphenolic contents as well as the phenolic profiles for CF and CL extracts were inspected using HPLC-DAD to explore the bioactive phytochemical compounds. Acute toxicity test showed that LD50 was 1500 and 2333 mg/kg b.wt. for CL and CF, respectively. The cytogenetic study was also done to detect the chromosome aberrations in somatic and germ cells after CL and CF administration to mice. DPPH antioxidant capacity assay revealed that CL has high redox-active effect than CF. This study is a contribution to the development of a creative new DDS that help in cancer treatment

Chemical Composition, Cytotoxic Activity And Antimicrobial Activity Of Essential Oils Of Leaves And Berries Of Juniperus Phoenice A L. Grown In Egypt.

Hydrodistillation of berries and leaves of Juniperus phoenicea grown in Sinai yielded volatile oils in the yield of 0.36 and 1.96%, respectively. Using gas chromatography/mass spectrometry technique, fifty eight compounds were identified in berry oil representing 99.2% of the oil composition. α-Pinene was the major compound in berry oil (39.30%) followed by sabinene (24.29%). Berry oil composed mainly of monoterpenoids which amounted to 90.53%, of which 72.85% was monoterpene hydrocarbons. The sesquiterpenoids accounted for about 8% of the total oil composition. Leaf oil was composed of about 66 compounds representing 99.16% of the total composition of the oil. α-Pinene was the major constituent of leaf oil at concentration of 38.22%, followed by α -cedrol (31.23%). The monoterpene hydrocarbon was the predominant chemical group (41.29%) followed by the oxygenated sesquiterpenes (32.21%). Both oils showed very high cytotoxic activities against all cell line tested. They showed equal activities against brain (0.6 μg//ml) and cervix (5.0 μg//ml) human cell lines, while berry oil was slightly more active than leaf oil against lung (0.6 and 0.7 μ/ml, respectively), liver (0.7 and 0.9 μg//ml, respectively) and breast human cell lines (0.8 and 1. μg//ml, respectively).The antimicrobial activity and minimum inhibitory concentration (MIC) of leaf and berry oils were also determined. The oils showed high activity against most of the tested strains